LOCUS       sequence-447732-       17953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for copper and flippase-regulated expression of BeYDV 
            Rep/RepA, including the TU for copper-regulated FLP, the TU for 
            constitutive expression of CUP2-GAL4, and the nptII cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17953)
  AUTHORS   Garcia-Perez E, Vazquez-Vilar M, Lozano-Duran R, Orzaez D
  TITLE     CuBe: a geminivirus-based copper-regulated expression system 
            suitable for post-harvest activation.
  JOURNAL   Plant Biotechnol J. 2025 Jan;23(1):141-155. doi: 10.1111/pbi.14485. 
            Epub 2024 Oct 22.
  PUBMED    39435699
REFERENCE   2  (bases 1 to 17953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14485";
            journalName: "Plant Biotechnol J"; date: "2025-01"; volume: "23"; 
            issue: "1"; pages: "141-155"
COMMENT     Sequence Label: pDGB3_alpha1
            nptII-DISTC02-CBS-FRT-Rep-RepA-tN...
FEATURES             Location/Qualifiers
     source          1..17953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(28..280)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(320..1114)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(431..450)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1041..1060
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1148..1327)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    2740..2773
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      2774..3481
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    3482..3515
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      4674..4926
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             6697..6717
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7401..7589
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      8202..8454
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        9777..9956
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      10947..11199
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    11602..11626
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12927..13556
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13985..15058
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      15124..15318
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(15554..15576)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    15662..15802
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15717..15736
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15817..15834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15988..16576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16068..16087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(16664..17479)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(16664..17479)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     17387..17406
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    17912..17936
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagagcgat cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata
       61 atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg
      121 ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta
      181 catgcttaac gtaattcaac agaaattata tgataatcat ggcaagaccg gcaacaggat
      241 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc tagctagagt
      301 ccgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gctatgcgct
      361 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
      421 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
      481 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
      541 aggcgtcgcc gtgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg
      601 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
      661 gaccggcttc catccgagta cgtgctcgct cgatgcggtg tttcgcttgg tggtcgaatg
      721 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
      781 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
      841 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
      901 tggccagcca cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt
      961 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     1021 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     1081 gagaacctgc gtgcaatcca tcttgttcaa tcatgcctcg atcgagttga gagtgaatat
     1141 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag
     1201 aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg
     1261 acgtatgtgc ttagctcatt aaactccaga aacccgcggc tcagtggctc cttcaacgtt
     1321 gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcatac
     1381 cgtgactccc ttaattctca tgtatctccg tcaggagtaa ttgtacatct ctgtaggctc
     1441 caatgtcaac ctctcgcatc tcctcactgg tgcgtatgtg ctccttctcc taacatgacc
     1501 aaaccatctg agtcgcgctt ctcgcatctt gtcctcaata ggggccacac ccaccttgtc
     1561 gcgaatagcc tcatttttta tcctatctaa cctggtgtac ccatacatct atctcaatat
     1621 tctcatcttt gctaccttca tcttctggaa ataagcgatg ttgactggcc aacactcaga
     1681 cccatgcaac attgtttgtc tgatcactac tctgtagaac ttaccgttaa gtttcagtgg
     1741 caccttcttg tcacacgaaa cactggaagc gagtctctat ttcatccatc ccgctccaat
     1801 acaatatgtg acatctccat caatctctcc atcaccctga ataatagacc caagatactt
     1861 aaaactccct atccttagga tggcatgcga gtccagcctc acctctcatt cctctccttg
     1921 agtctctcca ctgaacttac actccaagta ttctgtcttg gtccttctca acttgaaacc
     1981 tttagattcc aaggtctgcc tccacacctc tatttgcgca ttcacaccat cttgcatctc
     2041 gtcaatcaat acaatatcat ctacaaatag catgcacctc gacacctctt cttggatgtg
     2101 gcgcgttagt acgcccatca ccaaagcaaa caaaaaagag ctgagtgccg acccctcatg
     2161 caaccccatc ataactgaaa aatggtttga gtccccaccc accgtcctca ctcgggtctt
     2221 tacttcatca tatatgtcct taatcaacct aacataggca acaagtacac ctctagcctc
     2281 caaacatctt cacaaaacct ccctcaaaac tttttcatat gctttttcta agtcgatgaa
     2341 tgtcatatgc aagtccttct ttccctccct atactgctct atcaatatcc taacaaggtg
     2401 gatggcttct gtagtcgcgc tgtcaggaga gtggtcttgg gagagcgatg cgtcttttcc
     2461 gatgaaccgt tccagcaaaa aagactaagc gatgcgtctt ttccgctgaa ccgttccagc
     2521 aaaaaagact aagcgatgcg tcttttccgc tgaaccgttc cagcaaaaaa gactaggaga
     2581 gcgatgcgtc ttttccgctg aaccgttcca gcaaaaaaga ctatcccgtc ctaatacctc
     2641 tacgtgcaag cagtataaaa acaaaataca taaacataac taaaatctca tatcgttgta
     2701 attgtcatat actagtggta attaagtcca gttacacttg aagttcctat tctctagaaa
     2761 gtataggaac ttcctgcttt aatgagatat gcgagaagcc tatgatcgca tgatatttgc
     2821 tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg
     2881 ccggtttcgg ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat
     2941 attctccgtt caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa
     3001 acaatatatt gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa
     3061 acaattgcgt tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc
     3121 taaaagactg attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct
     3181 acgacacacc gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg
     3241 cgcgcatggg tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg
     3301 ggcaccattc aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt
     3361 atgcagcatt tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt
     3421 gacgacaaat cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca
     3481 ggaagttcct attctctaga aagtatagga acttcaaggt taaatttgct gactcttttt
     3541 tacctttcat ttctgaaaaa tgccttctgc tagtaagaac ttcagactcc aatctaaata
     3601 tgttttcctt acctatccca agtgctcatc tcaaagagat gatttattcc agtttctctg
     3661 ggagaaactc acaccttttc ttattttctt ccttggtgtt gcttctgagc ttcatcaaga
     3721 tggcactacc cactatcatg ctcttctcca gcttgataaa aaaccttgta ttagggatcc
     3781 ttcttttttc gattttgaag gaaatcaccc taatatccag ccagctagaa actctaaaca
     3841 agtccttgat tacatatcaa aggacggaga tattaaaacc agaggagatt tccgagatca
     3901 taaggtttct cctcgcaaat ctgacgcacg atggagaact attatccaga ctgcaacgtc
     3961 taaggaggaa tatcttgaca tgatcaagga agaattccct catgaatggg caacaaagct
     4021 tcaatggctg gaatattcag ccaacaaatt attccctcca caacctgaac cgtatgtgtc
     4081 gcccttcaca gaatcagatc ttcgctgcca cgaagatcta cactcctgga gggaaaccca
     4141 tctataccat gtaagcatag acgcttatac ttacatacat cctgtctcat accaacaagc
     4201 tcaatctgac cttgaatgga tggccgattt aaccaggaca atggaaggaa tggaatccga
     4261 caccccagcc tctacatctg cggaccaact cgtaccggaa agaccacctg ggctagaagt
     4321 ctcggacgac acaactattg gaacggtacc atcgatttca ccaactacga tgaacacgcc
     4381 acctataata tcatcgacga catccccttc aagttcgtcc cattgtggaa gcaattaata
     4441 ggttgccagt ctgatttcac tgtcaaccct aaatatggaa aaaagaagaa aataaaaggt
     4501 gggatccctt ctataattct ttgcaatcct gacgaagact ggatgttatc aatgacaagt
     4561 caacagaagg attactttaa agataattgc gtcacccact acatgtgtga cggggagact
     4621 ttttttgctc gggaatcgtc gagtcactga gcttggaatg gatcttcgat cccgatcgtt
     4681 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgacgatta
     4741 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     4801 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     4861 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
     4921 tagatcggga attgccaagc taattcttga agacgaaagg gcctcgtgat acgcctattt
     4981 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5041 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5101 atgagacaat aaccctgata aatgcttcaa taatgggacc gactcgcgct gtcaggagtt
     5161 attgaaataa aagctcatcg tcatttgatc gaggtcaaac ctttctcttt tttggcgaaa
     5221 gcccttgacc ttaaagggta ttattttgaa cttatcgaaa taacagcccg tcgtcgttcg
     5281 atcgatgtcg aacctttctc tctttttttg caaaggcctt tggccttaaa gggtgttact
     5341 tcgtacatat cgagataaca acttgtcgtc gttcaatcga ggtcgaacct ttcccttttt
     5401 tggcgaaggc cctcgtcctt gaagggtgtt attttgaact tatcgaaata acagcccgtt
     5461 gtcattcgat cgaggtctaa cctttctatt ttttggcgaa ggccctctta tcgaaataat
     5521 agctcgttgt cgttctatag aggtcgaacc cttctctttt ttggtgaagg cccttggcct
     5581 taaagggtgt tattaacctc tcgaaataac agcccgccgt cgttcgatca aggttgaacc
     5641 tttctctttt ttggcaaagg cccttggctt taaagggtgt tatttcaaac ttatcgaaat
     5701 aataagctcg tcgtcagtcc cattttcttg agagttggac atcttccgtg gggttcctag
     5761 tttgtttgat attgctttta ccagatggtg caacgctggt gaatacaagg tgttgacgtt
     5821 tcgtttatgt tcgtgttgcg tacgcattat ggttttcatg tctgactctt gccgtccagc
     5881 tcggatacgt tgctggcagg atgtatttca actatgttga agcaattttt gtccttcaac
     5941 tgaaatgttc ctttgtacat tcttccacgc gggtctaatg tccttgcttg gttaaagggt
     6001 gggaggtgag gatgatattt tcttcgctca cgccctgtgg ttcggtgggg tataacggat
     6061 tggtaacact atttgccttg tttagtatat atatggttga tggggcgagg atttctaagc
     6121 ttggtaatcc tactcggctc tccttccctt actgcgctgc tccgctgtca ggagtctcaa
     6181 tggtaacttt actctttatt taaccataca ttttttttta tttttttcac tttgttcttc
     6241 atccactatt gttctttgtt catcttgaac aaaagctccc tccttctttg ttcttcatcc
     6301 accattgttc ttcatcaatc atttcgctgt caggagagtg gtcttgggag agcgatgcgt
     6361 cttttccgat gaaccgttcc agcaaaaaag actaagcgat gcgtcttttc cgctgaaccg
     6421 ttccagcaaa aaagactaag cgatgcgtct tttccgctga accgttccag caaaaaagac
     6481 taggagagcg atgcgtcttt tccgctgaac cgttccagca aaaaagacta tcccgtccta
     6541 atacctctac gtgcaagcag tataaaaaca aaatacataa acataactaa aatctcatat
     6601 cgttgtaatt gtcatatact agtggtaatt aagtccagtt acacttaagg ttaaatttgc
     6661 tgactctttt ttacctttca tttctgaaaa atggctccta agaagaagag gaaggtgatg
     6721 agccagttcg acatcctgtg caagaccccc cccaaggtgc tggtgcggca gttcgtggag
     6781 agattcgaga ggcccagcgg cgagaagatc gccagctgtg ccgccgagct gacctacctg
     6841 tgctggatga tcacccacaa cggcaccgcc atcaagaggg ccaccttcat gagctacaac
     6901 accatcatca gcaacagcct gagcttcgac atcgtgaaca agagcctgca gttcaagtac
     6961 aagacccaga aggccaccat cctggaggcc agcctgaaga agctgatccc cgcctgggag
     7021 ttcaccatca tcccttacaa cggccagaag caccagagcg acatcaccga catcgtgtcc
     7081 agcctgcagc tgcagttcga gagcagcgag gaggccgaca agggcaacag ccacagcaag
     7141 aagatgctga aggccctgct gtccgagggc gagagcatct gggagatcac cgagaagatc
     7201 ctgaacagct tcgagtacac cagcaggttc accaagacca agaccctgta ccagttcctg
     7261 ttcctggcca cattcatcaa ctgcggcagg ttcagcgaca tcaagaacgt ggaccccaag
     7321 agcttcaagc tggtgcagaa caagtacctg ggcgtgatca ttcagtgcct ggtgaccgag
     7381 actaagacaa gcgtgtccag gtaagtttct gcttctacct ttgatatata tataataatt
     7441 atcattaatt agtagtaata taatatttca aatatttttt tcaaaataaa agaatgtagt
     7501 atatagcaat tgcttttctg tagtttataa gtgtgtatat tttaatttat aacttttcta
     7561 atatatgacc aaaatttgtt gatgtgcagg cacatctact ttttcagcgc cagaggcagg
     7621 atcgaccccc tggtgtacct ggacgagttc ctgaggaaca gcgagcccgt gctgaagaga
     7681 gtgaacagga ccggcaacag cagcagcaac aagcaggagt accagctgct gaaggacaac
     7741 ctggtgcgca gctacaacaa ggccctgaag aagaacgccc cctaccccat cttcgctatc
     7801 aagaacggcc ctaagagcca catcggcagg cacctgatga ccagctttct gagcatgaag
     7861 ggcctgaccg agctgacaaa cgtggtgggc aactggagcg acaagagggc ctccgccgtg
     7921 gccaggacca cctacaccca ccagatcacc gccatccccg accactactt cgccctggtg
     7981 tccaggtact acgcctacga ccccatcagc aaggagatga tcgccctgaa ggacgagact
     8041 aaccccatcg aggagtggca gcacatcgag cagctgaagg gcagcgccga gggcagcatc
     8101 agataccccg cctggaacgg catcatcagc caggaggtgc tggactacct gagcagctac
     8161 atcaacaggc ggatctgagc ttggaatgga tcttcgatcc cgatcgttca aacatttggc
     8221 aataaagttt cttaagattg aatcctgttg ccggtcttgc gacgattatc atataatttc
     8281 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     8341 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     8401 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
     8461 tgccaagcta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     8521 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     8581 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8641 ccctgataaa tgcttcaata atgggaccga ctcgcgctgt caggagtcta ttgtcaatta
     8701 tcttaaagta gtagtaggta agaattggat aattaatttc attctttctc tatatcaaca
     8761 acaaatttca tgtttaagtg taaaacttta aaatagatta aattgcattg gttattgtag
     8821 aatcgattat ttaactggtg gcaattggac tgaggcctat gtgttggctc aagaaatttt
     8881 aaggtgagtt gatataacac ctttactaaa gtgatttaac tcacaaatgc acgcatacaa
     8941 ggtatttgat aaatttcttt aaagagttta tatttactta actggagcga attagtacct
     9001 attaacttag aattgttcta gcaaaagttt aaataattta ttatgatata tgtgcataaa
     9061 ttttcagatc tttgtgttat tcttatatga tattttcatg ttgaaaattt atcaagaagc
     9121 aagaatattt agaaatactt ttatatgttt atttcattct tagtcatgct ttacagttaa
     9181 ggataccagc atgagcatat atatgaggtt ctataaagag ttagacttga aaagtcacaa
     9241 gaagaagttg tagaaagaaa aaaattttgg gagtatcctt tattctgata aggagtcatc
     9301 gtcctgacca aggcgttgac ttcctcaaac tacctgtgcc aaagtaggga gcacacgaac
     9361 tgaaggtcta gtttctgaaa atttacttag ccatgttaag gtatgataca tgagcgttga
     9421 gaaccatgaa acgagtggca cattgtggct tgggcctatt cgatcagttt gggatcgaac
     9481 ccgtatcgat catacggtga ctgagacaga attaagtcag aatagttgga actcgcaaag
     9541 tataaagtaa agtatttttt taataagaaa gaaaaaacaa tttcttttag aatattcata
     9601 aattgttata atgcaattat tttagaattg ttcttataaa ctttatgtct tatatgcctt
     9661 tagaatcttt gcttgtaatg tgtatgcgct gtcaggagat acatgagaat taagggagtc
     9721 acggtatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac
     9781 cgcaacgttg aaggagccac tgagccgcgg gtttctggag tttaatgagc taagcacata
     9841 cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata
     9901 tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt
     9961 ctcatattca ctctcaactc gatcgaggca atgatggtcg taattaacgg ggtcaaatat
    10021 gcctgtgaaa cgtgtatcag gggtcacagg gcggcgcagt gtactcacac tgatggtccg
    10081 ctacagatga tcagacgcaa gggaagacca tcgaccacat gtggccattg taaagagctg
    10141 agaagaacca agaacttcaa cccatccggt gggtgcatgt gtgcctctgc acgacggcca
    10201 gctgttggca gcaaggaaga tgaaacacga tgtcgttgtg atgagggtga accttgtaaa
    10261 tgtcatacca agaggaaaag cagccggaaa tcaaagggag ggtcatgcca cagaagggca
    10321 aatgatgaag cagcgcatgt caatggtctt ggtattgcag atctggacgt tcttttgggc
    10381 ctaaatggtc gctcgtcgga tgtagacatg acaaccacat tgccgagttt gaagccacct
    10441 ctgcaaaacg gagaaattaa ggccgacagc attgacaatc ttgatttggc ttccctcgat
    10501 ccgcttgagc aaagccctag tatatctatg gaacctgtta gtatcaatga aacaggaagc
    10561 gcatatacaa ctacgaacac agcactaaac gatattgaca ttccattctc catcaatgag
    10621 ttgaacgagc tatacaaaca agtatcttcg cataactcac attcacagcc aacaaattct
    10681 caagcgcttt cacaaccaat tgcctcctct aacgttcatg ataacttcat gaataatgaa
    10741 atcacggcta gtaaaattga tgatggtaat aattcaaaac cactgtcacc tggttggacg
    10801 gaccaaactg cgtataacgc gtttggaatc actacaggga tgtttaatac cactacaatg
    10861 gatgatgtat ataactatct attcgatgat gaagataccc caccaaaccc aaaaaaagag
    10921 tgagcttgga atggatcttc gatcccgatc gttcaaacat ttggcaataa agtttcttaa
    10981 gattgaatcc tgttgccggt cttgcgacga ttatcatata atttctgttg aattacgtta
    11041 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
    11101 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
    11161 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattgcca agctaattct
    11221 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
    11281 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    11341 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    11401 caataatggg accgactcgc gctgtcagga gtctcaatgg taactttact ctttatttaa
    11461 ccatacattt ttttttattt ttttcacttt gttcttcatc cactattgtt ctttgttcat
    11521 cttgaacaaa agctccctcc ttctttgttc ttcatccacc attgttcttc atcaatcatt
    11581 tcgctgtcat gagacgaatt ctgacaggat atattggcgg gtaaacctaa gagaaaagag
    11641 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
    11701 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
    11761 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat
    11821 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
    11881 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
    11941 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
    12001 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
    12061 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
    12121 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
    12181 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
    12241 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
    12301 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
    12361 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
    12421 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
    12481 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
    12541 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
    12601 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
    12661 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
    12721 gcgcacctct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
    12781 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
    12841 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
    12901 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
    12961 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
    13021 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
    13081 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
    13141 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
    13201 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
    13261 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
    13321 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
    13381 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
    13441 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
    13501 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
    13561 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
    13621 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
    13681 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
    13741 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
    13801 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
    13861 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
    13921 taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat
    13981 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
    14041 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
    14101 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
    14161 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
    14221 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
    14281 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
    14341 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
    14401 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
    14461 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
    14521 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
    14581 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
    14641 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
    14701 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
    14761 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
    14821 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
    14881 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
    14941 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
    15001 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
    15061 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggactctt
    15121 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
    15181 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
    15241 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
    15301 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc
    15361 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
    15421 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
    15481 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
    15541 atgacggtga aaacctctga cacatgcagc tcccggtgac ggtcacagct tgtctgtaag
    15601 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    15661 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
    15721 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    15781 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    15841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    15901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    15961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    16021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    16081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    16141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    16201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    16261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    16321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    16381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    16441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    16501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    16561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    16621 cgaaaactca cgttaaggga ttttggtcat gcattctagg tgattagaaa aactcatcga
    16681 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
    16741 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
    16801 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
    16861 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
    16921 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
    16981 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagtcgaa
    17041 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
    17101 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
    17161 atgctgtttt ccctgggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
    17221 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
    17281 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
    17341 gcttcccata caatcggtag attgtcgcac ctgattgccc gacattatcg cgagcccatt
    17401 tatacccata taaatcagca tccatgttgg aatttaatcg cggccttgag caagacgttt
    17461 cccgttgaat atggctcata acagaactta ttatttcctt cctcttttct acagtattta
    17521 aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc
    17581 taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac
    17641 atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat
    17701 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
    17761 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
    17821 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
    17881 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacataa
    17941 cgaattcgtc tca
//
