LOCUS       sequence-447736-        9428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Geminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + 
            eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + 
            BeYDV LIR1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Garcia-Perez E, Vazquez-Vilar M, Lozano-Duran R, Orzaez D
  TITLE     CuBe: a geminivirus-based copper-regulated expression system 
            suitable for post-harvest activation.
  JOURNAL   Plant Biotechnol J. 2025 Jan;23(1):141-155. doi: 10.1111/pbi.14485. 
            Epub 2024 Oct 22.
  PUBMED    39435699
REFERENCE   2  (bases 1 to 9428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14485";
            journalName: "Plant Biotechnol J"; date: "2025-01"; volume: "23"; 
            issue: "1"; pages: "141-155"
COMMENT     Sequence Label: pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP
            ...
FEATURES             Location/Qualifiers
     source          1..9428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(437..439,440..1150)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(437..439,440..1150)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(479..500)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(740..759)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1087..1108
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        2167..2511
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2455..2475
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    3072..3096
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4397..5026
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5455..6528
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6594..6788
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7024..7046)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7132..7272
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7187..7206
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7287..7304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7458..8046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7538..7557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8134..8949)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8134..8949)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8857..8876
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9382..9406
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagtagcag aaggcatgtt gttgtgactc cgaggggttg cctcaaactc tatcttataa
       61 ccggcgtgga ggcatggagg caggggtatt ttggtcattt taatagatag tggaaaatga
      121 cgtggaattt acttaaagac gaagtctttg cgacaagggg gggcccacgc cgaatttaat
      181 attaccggcg tggccccccc ttatcgcgag tgctttagca cgagcggtcc agatttaaag
      241 tagaaaattt cccgcccact agggttaaag gtgttcacac tataaaagca tatacgatgt
      301 gatggtattt gatggagcgt atattgtatc aggtatttcc gttggatacg aattattcgt
      361 acgaccctcc taattaacct gactcaaaat ctaccctcat aattgttgtt tgatattggt
      421 cttgtatttt gcaggtgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      481 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      541 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      601 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
      661 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      721 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      781 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      841 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      901 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      961 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1021 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     1081 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1141 gctgtacaag gacgagctgt aagcttcggc catgctagag tccgcaaaaa tcaccagtct
     1201 ctctctacaa atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga
     1261 taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     1321 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     1381 atccagtgac ctataaaatg attattttat gaatatattt cattgtgcaa gtagatagaa
     1441 attacatatg ttacataaca cacgaaataa acaaaaaaag acaatccaaa aacaaacacc
     1501 ccaaaaaaaa taatcacttt agataaactc gtatgaggag aggcacgttc aggtaactag
     1561 agccaagctg atctcctttg ccccggagat caccatggac gactttctct atctctacga
     1621 tctaggaaga aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa
     1681 gattagcctc ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc
     1741 ggcaggtctg atcaagacga tctacccgag taataatctc caggagatca aataccttcc
     1801 caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa
     1861 agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca
     1921 taaaccaagg caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa
     1981 ggccatggag tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga
     2041 acagttcata cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt
     2101 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     2161 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     2221 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     2281 tcattgcgat aaaggaaagg ctatcgttca agatgcccct gccgacagtg gtcccaaaga
     2341 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     2401 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     2461 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt
     2521 tttacaacaa ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt
     2581 cgaagaatgt caggtaagcc atcttcctgc ttatttttat aatgaacata gaaataggaa
     2641 gttgtgcaga gaaacgctgt caggaggtgc caagcttgca tgcctgcagg tagcagaagg
     2701 catgttgttg tgactccgag gggttgcctc aaactctatc ttataaccgg cgtggaggca
     2761 tggaggcagg ggtattttgg tcattttaat agatagtgga aaatgacgtg gaatttactt
     2821 aaagacgaag tctttgcgac aagggggggc ccacgccgaa tttaatatta ccggcgtggc
     2881 ccccccttat cgcgagtgct ttagcacgag cggtccagat ttaaagtaga aaatttcccg
     2941 cccactaggg ttaaaggtgt tcacactata aaagcatata cgatgtgatg gtatttgatg
     3001 gagcgtatat tgtatcaggt atttccgttg gatacgaatt attcgtacga ccctccgctt
     3061 gagacgaagc ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     3121 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     3181 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     3241 agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat gtcgcacaag
     3301 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     3361 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     3421 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     3481 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     3541 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     3601 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     3661 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     3721 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     3781 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     3841 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     3901 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     3961 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     4021 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     4081 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     4141 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacctct
     4201 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     4261 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     4321 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     4381 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     4441 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     4501 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     4561 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     4621 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     4681 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     4741 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     4801 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     4861 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     4921 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     4981 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     5041 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     5101 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     5161 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     5221 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     5281 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     5341 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     5401 ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     5461 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     5521 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     5581 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     5641 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     5701 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     5761 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     5821 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     5881 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     5941 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     6001 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     6061 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     6121 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     6181 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     6241 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     6301 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     6361 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     6421 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     6481 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     6541 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggactctt tcctgtggat
     6601 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     6661 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     6721 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     6781 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     6841 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     6901 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     6961 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     7021 aaacctctga cacatgcagc tcccggtgac ggtcacagct tgtctgtaag cggatgccgg
     7081 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     7141 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     7201 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     7261 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7321 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7381 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7441 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7501 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7561 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7621 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7681 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7741 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7801 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7861 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7921 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7981 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8041 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8101 cgttaaggga ttttggtcat gcattctagg tgattagaaa aactcatcga gcatcaaatg
     8161 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     8221 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     8281 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     8341 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
     8401 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     8461 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagtcgaa atacgcgatc
     8521 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     8581 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     8641 ccctgggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     8701 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     8761 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     8821 caatcggtag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     8881 taaatcagca tccatgttgg aatttaatcg cggccttgag caagacgttt cccgttgaat
     8941 atggctcata acagaactta ttatttcctt cctcttttct acagtattta aagatacccc
     9001 aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta
     9061 aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga
     9121 cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc
     9181 aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct
     9241 tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac
     9301 gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
     9361 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacataa caagcttcgt
     9421 ctcagtca
//
