LOCUS       sequence-447738-       10141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV LIR1 + TU 35S:eGFP:T35S + BeYDV SIR-LIR2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Garcia-Perez E, Vazquez-Vilar M, Lozano-Duran R, Orzaez D
  TITLE     CuBe: a geminivirus-based copper-regulated expression system 
            suitable for post-harvest activation.
  JOURNAL   Plant Biotechnol J. 2025 Jan;23(1):141-155. doi: 10.1111/pbi.14485. 
            Epub 2024 Oct 22.
  PUBMED    39435699
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14485";
            journalName: "Plant Biotechnol J"; date: "2025-01"; volume: "23"; 
            issue: "1"; pages: "141-155"
COMMENT     Sequence Label: pDGB3_alpha1_LIR1-35S-eGFP-T35S-SIR-LIR2 (GB4312)
FEATURES             Location/Qualifiers
     source          1..10141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1199..1543
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1487..1507
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1617..1619,1620..1622,1623..2333)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1617..1619,1620..1622,1623..2333)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1662..1683)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1923..1942)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2270..2291
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3790..3814
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5115..5744
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6173..7246
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7312..7506
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7742..7764)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7850..7990
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7905..7924
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8005..8022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8176..8764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8256..8275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8852..9667)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8852..9667)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9575..9594
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    10100..10124
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggaggtgcca agcttgcatg cctgcaggta gcagaaggca tgttgttgtg actccgaggg
       61 gttgcctcaa actctatctt ataaccggcg tggaggcatg gaggcagggg tattttggtc
      121 attttaatag atagtggaaa atgacgtgga atttacttaa agacgaagtc tttgcgacaa
      181 gggggggccc acgccgaatt taatattacc ggcgtggccc ccccttatcg cgagtgcttt
      241 agcacgagcg gtccagattt aaagtagaaa atttcccgcc cactagggtt aaaggtgttc
      301 acactataaa agcatatacg atgtgatggt atttgatgga gcgtatattg tatcaggtat
      361 ttccgttgga tacgaattat tcgtacgacc ctccctaaga ttcttgattg tttcgctgtc
      421 aggagtctca atggtaactt tactctttat ttaaccatac attttttttt atttttttca
      481 ctttgttctt catccactat tgttctttgt tcatcttgaa caaaagctcc ctccttcttt
      541 gttcttcatc caccattgtt cttcatcaat catttcgctg tcaggagact agagccaagc
      601 tgatctcctt tgccccggag atcaccatgg acgactttct ctatctctac gatctaggaa
      661 gaaagttcga cggagaaggt gacgatacca tgttcaccac cgataatgag aagattagcc
      721 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcggcaggtc
      781 tgatcaagac gatctacccg agtaataatc tccaggagat caaatacctt cccaagaagg
      841 ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat
      901 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
      961 ggcaagtaat agagattgga gtctctaaga aagtagttcc tactgaatca aaggccatgg
     1021 agtcaaaaat tcagatcgag gatctaacag aactcgccgt gaagactggc gaacagttca
     1081 tacagagtct tttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg
     1141 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg
     1201 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
     1261 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
     1321 ataaaggaaa ggctatcgtt caagatgccc ctgccgacag tggtcccaaa gatggacccc
     1381 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     1441 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
     1501 acccttcctc tatataagga agttcatttc atttggagag gactccggta tttttacaac
     1561 aattaccaca acaaaacaaa caacaaacaa cattacaatt tactattcta gtcgaaatgg
     1621 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1681 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1741 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1801 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1861 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1921 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1981 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2041 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     2101 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     2161 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2221 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2281 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggacgagc
     2341 tgtaagcttc ggccatgcta gagtccgcaa aaatcaccag tctctctcta caaatctatc
     2401 tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc
     2461 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     2521 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctcgctg
     2581 tcaggaggag tgtacttcaa gtcagtggga aatcaataaa atgattattt tatgaatata
     2641 tttcattgtg caagtagata gaaattacat atgttacata acacacgaaa taaacaaaaa
     2701 aagacaatcc aaaaacaaac accccaaaaa aaataatcac tttagataaa ctcgtatgag
     2761 gagaggcacg ttcagtgact cgacgattcc cgagcaaaaa aagtctcccc gtcacacatg
     2821 tagtgggtga cgcaattatc tttaaagtaa tccttctgtt gacttgtcat tgataacatc
     2881 cagtcttcgt caggattgca aagaattata gaagggatcc cactcgagcg ctgtcaggag
     2941 ggagaggtaa gtttcacttc acacattatt actgtcttct aatacaaggt tttttatcaa
     3001 gctggagaag agcatgatag tgggtagtgc catcttgatg aagctcagaa gcaacaccaa
     3061 ggaagaaaat aagaaaaggt gtgagtttct cccagagaaa ctggaataaa tcatctcttt
     3121 gagatgagca cttgggatag gtaaggaaaa catatttaga ttggagtctg aagttcttac
     3181 tagcagaagg catgttgttg tgactccgag gggttgcctc aaactctatc ttataaccgg
     3241 cgtggaggca tggaggcagg ggtattttgg tcattttaat agatagtgga aaatgacgtg
     3301 gaatttactt aaagacgaag tctttgcgac aagggggggc ccacgccgaa tttaatatta
     3361 ccggcgtggc ccccccttat cgcgagtgct ttagcacgag cggtccagat ttaaagtaga
     3421 aaatttcccg cccactaggg ttaaaggtgt tcacactata aaagcatata cgatgtgatg
     3481 gtatttgatg gagcgtatat tgtatcaggt atttccgttg gatacgaatt attcgtacga
     3541 ccctccctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg
     3601 gtcatagcgc tgtcaggagt ctcaatggta actttactct ttatttaacc atacattttt
     3661 ttttattttt ttcactttgt tcttcatcca ctattgttct ttgttcatct tgaacaaaag
     3721 ctccctcctt ctttgttctt catccaccat tgttcttcat caatcatttc gctgtcatga
     3781 gacgaattct gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     3841 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     3901 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     3961 tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt cgcacaagtc
     4021 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     4081 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     4141 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     4201 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     4261 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     4321 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     4381 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     4441 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     4501 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     4561 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     4621 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     4681 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     4741 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     4801 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     4861 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacctctca
     4921 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     4981 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     5041 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     5101 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     5161 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     5221 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     5281 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     5341 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     5401 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     5461 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     5521 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     5581 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     5641 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     5701 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     5761 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     5821 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     5881 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     5941 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     6001 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     6061 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     6121 ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg
     6181 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
     6241 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
     6301 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
     6361 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
     6421 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
     6481 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
     6541 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
     6601 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
     6661 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
     6721 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
     6781 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
     6841 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
     6901 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac
     6961 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc
     7021 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag
     7081 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa
     7141 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat
     7201 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat
     7261 gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggactctttc ctgtggatag
     7321 cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc
     7381 aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg
     7441 cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg
     7501 tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc
     7561 gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa
     7621 aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact
     7681 cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa
     7741 acctctgaca catgcagctc ccggtgacgg tcacagcttg tctgtaagcg gatgccggga
     7801 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     7861 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     7921 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     7981 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8041 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8101 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8161 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8221 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8281 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8341 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8401 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8461 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8521 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8581 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8641 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8701 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8761 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8821 ttaagggatt ttggtcatgc attctaggtg attagaaaaa ctcatcgagc atcaaatgaa
     8881 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     8941 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     9001 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     9061 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     9121 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     9181 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagtcgaaat acgcgatcgc
     9241 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     9301 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     9361 ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     9421 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     9481 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     9541 atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     9601 aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc cgttgaatat
     9661 ggctcataac agaacttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     9721 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     9781 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     9841 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     9901 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     9961 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
    10021 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
    10081 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacataacg aattcgtctc
    10141 a
//
