LOCUS       sequence-447745-       10050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DONOR vector for genomic integration of MARINA voltage sensitive 
            indicator gene from Platisa et al., 2017 
            (10.1021/acschemneuro.6b00234)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Petruskeviciute A, Simuliunaite U, Polanco CM, Rojas B, Kuras S, 
            Valatkaite-Rakstiene B, Norvilas R, Vijaya AK, Munoz P, Neniskyte U,
            Jakubauskas A, Burokas A, Nalvarte I, Inzunza J, Naumovas D, Stoskus
            M, Griskevicius L, Baltriukiene D, Arias J
  TITLE     Generation of a genetically encoded voltage indicator MARINA 
            reporter human iPS cell line using Cas9 (VULSCi002-A-2).
  JOURNAL   Stem Cell Res. 2024 Dec 12;82:103628. doi: 
            10.1016/j.scr.2024.103628.
  PUBMED    39721357
REFERENCE   2  (bases 1 to 10050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2024-12-12"; volume: "82:103628. doi"; pages: " 
            10.1016/j.scr.2024.103628"
COMMENT     Sequence Label: pDONOR-MARINA
FEATURES             Location/Qualifiers
     source          1..10050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(126..194,195..986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(344..363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1899..1916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2069..2074,2075..2092,2093..2099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2112..2134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2131..2147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    2199..3002
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    3009..3034
                     /label=SA
                     /note="splice acceptor site"
     CDS             3058..3111
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3121..3720
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3121..3140)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3617..3637
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(3752..3769)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3758..3982
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3986..4365
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4367..4643
                     /label=chicken beta-actin promoter
     intron          4644..5660
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5584..5606
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5668..5687
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      5713..5722
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7195..7248
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7270..7290
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7291..7998
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7433..7451)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7701..7720
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7895..7918
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(8077..8096)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8142..8197
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8196..8215)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    8731..9260
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(9406..9425)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9407..9425)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9432..9449)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9432..9448)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9441..9463)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9590..10045
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9677..9696)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9887..9908
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcaaac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agctcgaaat taaccctcac taaagggaac aaaagctgtg ctttctctga ccagcattct
     2221 ctcccctggg cctgtgccgc tttctgtctg cagcttgtgg cctgggtcac ctctacggct
     2281 ggcccagatc cttccctgcc gcctccttca ggttccgtct tcctccactc cctcttcccc
     2341 ttgctctctg ctgtgttgct gcccaaggat gctctttccg gagcacttcc ttctcggcgc
     2401 tgcaccacgt gatgtcctct gagcggatcc tccccgtgtc tgggtcctct ccgggcatct
     2461 ctcctccctc acccaacccc atgccgtctt cactcgctgg gttccctttt ccttctcctt
     2521 ctggggcctg tgccatctct cgtttcttag gatggccttc tccgacggat gtctcccttg
     2581 cgtcccgcct ccccttcttg taggcctgca tcatcaccgt ttttctggac aaccccaaag
     2641 taccccgtct ccctggcttt agccacctct ccatcctctt gctttctttg cctggacacc
     2701 ccgttctcct gtggattcgg gtcacctctc actcctttca tttgggcagc tcccctaccc
     2761 cccttacctc tctagtctgt gctagctctt ccagccccct gtcatggcat cttccagggg
     2821 tccgagagct cagctagtct tcttcctcca acccgggccc ctatgtccac ttcaggacag
     2881 catgtttgct gcctccaggg atcctgtgtc cccgagctgg gaccacctta tattcccagg
     2941 gccggttaat gtggctctgg ttctgggtac ttttatctgt cccctccacc ccacagtggg
     3001 gcaagcttct gacctcttct cttcctccca cagggcctcg agagatctgg cagcggagag
     3061 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc taggctcgag
     3121 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     3181 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     3241 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     3301 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     3361 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     3421 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     3481 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     3541 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     3601 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     3661 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     3721 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3781 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3841 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3901 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3961 ggggatgcgg tgggctctat gggtcgacat tgattattga ctagttatta atagtaatca
     4021 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     4081 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     4141 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     4201 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     4261 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     4321 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     4381 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     4441 attttttaat tattttgtgc agcgatgggg gcgggggggg gggggggsgc gcgccagggg
     4501 gggggggggg gggggagggg sggggsgggg sgaggsggag aggtgcggcg gcagccaatc
     4561 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     4621 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
     4681 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     4741 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
     4801 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
     4861 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
     4921 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     4981 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     5041 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
     5101 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     5161 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     5221 aggkgggggt gccgggcggg gcggggccgc cycgggccgg ggrgggctcg ggggrggggc
     5281 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     5341 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     5401 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     5461 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     5521 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     5581 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     5641 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     5701 ttggcaaagg ccgccaccat ggaaggtttt gacgggtcag actttagccc tcctgccgat
     5761 ctcgtgggtg tggacggtgc tgtgatgcgg aacgtggtgg acgtgacaat caacggcgac
     5821 gtgactgcac cacctaaggc agctcctaga aaatctgaga gtgtgaagaa agtccactgg
     5881 aacgacgtgg atcagggacc aagcgagaag cccgaaacaa gacaggagaa gagcaggatc
     5941 accagcgagg gcgagtacat ccccctggac cagatcgaca tcaacgtgga cgacgggagg
     6001 atggaaattc ctaccacagg agtgggtaga gtccagttta gggtgagagc tgtcatcgac
     6061 catctgggaa tgcgggtgtt cggcgtcttt ctcatcttcc tggacatcat tctgatgatc
     6121 attgatctga gtctccctgg gaagtcagag agctcccaga gcttctacga cggcatggca
     6181 ctggccctca gctgctactt catgctggat ctcggcctga ggatcttcgc atacggaccc
     6241 aagaacttct ttaccaatcc ttgggaggtg gccgacggac tgatcattgt ggtcactttt
     6301 gtggtcacca ttttctacac agtgctggac gagtacgtgc aggagacagg agcagacgga
     6361 ctcggacagc tggtggtcct cgctcgactg ctccgagtgg tccgactggc aaggatcttt
     6421 tactcccacc agcaggggaa ttcaatgtct aaaggggagg aactgttcac tggtgtggtc
     6481 cccattctgg tggagctcga cggcgacgtg aacggccata agttttcagt gagcggggag
     6541 ggtgaaggcg acgctactta cggaaagctc accctgaaat tcatctgcac taccgggaag
     6601 ctgccagtgc cttggcctac cctggtcaca actctcacat acggcgtgca gtgtttcagt
     6661 cgctatcctg accatatgaa gcgacacgat ttctttaaat ctgctatgcc agagggctac
     6721 gtgcaggaac ggaccatctt ctttaaggac gacggcaatt ataaaactcg cgcagaggtg
     6781 aagtttgaag gcgacaccct ggtcaaccga atcgagctca agggaattga cttcaaagaa
     6841 gacggaaata tcctggggca caagctcgag tacaactata acgccgctca ggtgtacatc
     6901 atggccgata agcagaaaaa cggcattaag gctaacttca agatccgcca caacattgaa
     6961 gacggcggag tgcagctggc cgatcattac cagcagaaca caccaatcgg agacgggcct
     7021 gtcctgctcc cagataatca cgttctgttc accacatcca ctctgtctaa ggaccccaac
     7081 gagaaaagag atcatatggt gctgctggag tttgtcactg ccgacgggat tacccacgga
     7141 atggacgagc tgtataagtt ctgctacgag aacgaggtgg catgcggaag cggagaagga
     7201 cggggtagtc tgctgacatg cggcgacgta gaagagaatc ctggtcccgc cccggggtcg
     7261 accatggtgc ccaagaagaa gaggaaagtc gtgagcaagg gcgaggagga caacatggcc
     7321 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     7381 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     7441 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     7501 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     7561 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     7621 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     7681 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     7741 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     7801 ctgaaggacg gcggccacta cgacgccgag gtcaagacca cctacaaggc caagaagccc
     7861 gtgcagctgc ccggcgccta caacgtcaac atcaagctgg acatcacctc ccacaacgag
     7921 gactacacca tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcggcatg
     7981 gacgagctgt acaagtaaca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     8041 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     8101 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     8161 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     8221 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     8281 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     8341 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     8401 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     8461 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     8521 atccctcgac ctgcagccca agcttggatc cctcgacagt actaagcttt gacagaaaag
     8581 ccccatcctt aggcctcctc cttcctagtc tcctgatatt gggtctaacc cccacctcct
     8641 gttaggcaga ttccttatct ggtgacacac ccccatttcc tggagccatc tctctccttg
     8701 ccagaacctc taaggtttgc ttacgatgga gccagagagg atcctgggag ggagagcttg
     8761 gcagggggtg ggagggaagg gggggatgcg tgacctgccc ggttctcagt ggccaccctg
     8821 cgctaccctc tcccagaacc tgagctgctc tgacgcggct gtctggtgcg tttcactgat
     8881 cctggtgctg cagcttcctt acacttccca agaggagaag cagtttggaa aaacaaaatc
     8941 agaataagtt ggtcctgagt tctaactttg gctcttcacc tttctagtcc ccaatttata
     9001 ttgttcctcc gtgcgtcagt tttacctgtg agataaggcc agtagccagc cccgtcctgg
     9061 cagggctgtg gtgaggaggg gggtgtccgt gtggaaaact ccctttgtga gaatggtgcg
     9121 tcctaggtgt tcaccaggtc gtggccgcct ctactccctt tctctttctc catccttctt
     9181 tccttaaaga gtccccagtg ctatctggga catattcctc cgcccagagc agggtcccgc
     9241 ttccctaagg ccctgctctg ggcttctggg tttgagtcct tggcaagccc aggagaggcg
     9301 ctcaggcttc cctgtccccc ttcctcgtcc accatctcat gcccctggct ctcctgcccc
     9361 ttccctacag gggttcctgg ctctgctcta gcgatcgcca attcgcccta tagtgagtcg
     9421 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     9481 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     9541 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     9601 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9661 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9721 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9781 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     9841 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     9901 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     9961 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    10021 aacaaaatat taacgcttac aatttaggtg
//
