LOCUS       sequence-44776-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F3-GCA
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..46
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             47..112
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tttttgcgga ctgccaccat gggctccaaa gaagaagcgt aaggtagact acaaagacca
       61 tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca agggtacccg
      121 cccatatgct tgccctgtcg agtcctgtga ccgccgcttc cgtcagagta gtcatctcaa
      181 gcagcacatc cgtatccaca ccggccagaa gcccttccag tgtcgcatct gcatgcgtaa
      241 cttcagtcgc tccagccacc tgaccaccca catccgcacc cacacaggcg agaagccttt
      301 tgcatgcgac atctgtggga ggaagtttgc ccagcgttct acccgcaaac ggcacaccaa
      361 gatccacaca ggtggatccc aactagtcaa aagtgaactg gaggagaaga aatctgaact
      421 tcgtcataaa ttgaaatatg tgcctcatga atatattgaa ttaattgaaa ttgccagaaa
      481 ttccactcag gatagaattc ttgaaatgaa ggtaatggaa ttttttatga aagtttatgg
      541 atatagaggt aaacatttgg gtggatcaag gaaaccggac ggagcaattt atactgtcgg
      601 atctcctatt gattacggtg tgatcgtgga tactaaagct tatagcggag gttataatct
      661 gccaattggc caagcagatg aaatgcaaga ctatgtcgaa gaaaatcaaa cacgaaacaa
      721 acatatcaac cctaatgaat ggtggaaagt ctatccatct tctgtaacgg aatttaagtt
      781 tttatttgtg agtggtcact ttaaaggaaa ctacaaagct cagcttacac gattaaatca
      841 tatcactaat tgtaatggag ctgttcttat tgtagaaaga gcttttaatt ggtggaga
//
