LOCUS       sequence-447846-        7029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses  MYC-tagged human TRAK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Davis K, Basu H, Izquierdo-Villalba I, Shurberg E, Schwarz TL
  TITLE     Miro GTPase domains regulate the assembly of the mitochondrial 
            motor-adaptor complex.
  JOURNAL   Life Sci Alliance. 2022 Oct 27;6(1):e202201406. doi: 
            10.26508/lsa.202201406. Print 2023 Jan.
  PUBMED    36302649
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2022-10-27"; volume: "6(1):e202201406. doi"; 
            pages: " 10.26508/lsa.202201406. Print 2023 Jan"
COMMENT     Sequence Label: MYC-hTRAK2
FEATURES             Location/Qualifiers
     source          1..7029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        218..421
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     372..392
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        467..485
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      517..526
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             526..555
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3377..3396)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3378..3396)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3670..3791
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3707..3726)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3761..3780
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3798..4253)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3935..3956)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4147..4166
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4280..4384
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4386..4743
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4386..4577
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4409..4429)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4594..4729
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4778..5572
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4778..5572
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4832..4851)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5442..5461
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5760..5779)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5804..5851
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6180..6768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6669..6688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        join(6943..7029,1..217)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
       61 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      121 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      181 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      241 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      301 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      361 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      421 tggtttagtg aaccgtcaga tccgctagcg attacgccaa gctcgaaatt aaccctcact
      481 aaagggaaca aaagctggag ctccaccgcg gtggcggccg ccatggagca gaaactcatc
      541 tctgaagagg atctggcccg ggcggatcca atgagtcaat cccagaatgc aatttttaca
      601 tcaccaacag gtgaagaaaa cctcatgaat agcaatcaca gagactcgga gagcatcact
      661 gatgtctgct ccaatgagga tctccctgaa gttgagctgg tgagtctgct agaagaacaa
      721 ctaccacagt ataggctaaa agtagacact ctctttctat atgaaaatca agactggact
      781 cagtctccac accagcggca gcatgcatct gatgctctct ctccagtcct tgctgaagag
      841 actttccgtt acatgattct aggcacagac agggtggagc agatgaccaa aacttacaat
      901 gacatcgaca tggttacaca tctcctggca gagagggatc gtgatctgga actcgctgct
      961 cgaattggac aagctctctt aaagcggaac catatcttat ctgagcagaa cgaatccctg
     1021 gaggagcaat tgggacaagc ctttgatcaa gttaatcagc tgcagcatga gctatgcaag
     1081 aaagatgagt tacttcgaat cgtctccatt gcttctgaag aaagtgaaac tgattccagc
     1141 tgttctacac ctcttcggtt caatgagtcc tttagcttat ctcaagggtt gctgcagttg
     1201 gaaatgctgc aagaaaagct caaggaactg gaagaagaga atatggctct tcgatccaag
     1261 gcttgtcaca taaagacaga aactgttacc tatgaagaaa aggaacaaca gcttgtcagc
     1321 gactgtgtta aagaacttcg tgaaacaaat gctcagatgt ccagaatgac tgaagaattg
     1381 tcagggaaga gtgatgagct ggttcgatac caagaagagc tttcctctct tttgtcacag
     1441 attgtagacc ttcagcataa acttaaagaa catgtgattg agaaggaaga actaaaactt
     1501 cacctgcaag cttccaaaga tgcccaacgg caactgacaa tggagctgca cgagttacaa
     1561 gacaggaata tggagtgtct aggaatgtta catgaatccc aagaagaaat aaaggaactt
     1621 cgtagtagat ctggccctac tgctcatctc tacttctccc aatcatatgg agcttttact
     1681 ggggaatctt tggcagctga gattgagggg actatgcgta aaaagctgag tttggatgag
     1741 gaatcttctc tctttaaaca aaaagcccaa cagaagcggg tatttgatac cgtcaggatt
     1801 gccaatgaca cacggggccg ctctatctca ttcccagctc tgttacccat tccaggctcc
     1861 aaccgttcaa gtgtcatcat gacagcaaaa ccttttgagt ctggtcttca gcaaacagag
     1921 gacaaatcac tcctgaacca ggggagcagc tcagaggagg ttgcagggag ctcccagaag
     1981 atgggccaac caggaccctc aggagatagt gatttggcta cagcactgca tcgccttagc
     2041 ttgcgtcgac aaaactattt aagtgagaag cagttctttg ctgaagaatg gcagcggaag
     2101 atccaggttc tggcagacca gaaggaagga gttagtggct gtgtcacccc gacagagagc
     2161 cttgcctctc tctgcaccac ccagtcagag atcacagacc tcagcagtgc cagttgcctt
     2221 cgaggtttta tgccagaaaa attacaaatt gtcaagcccc ttgaaggatc acaaactctg
     2281 tatcactggc agcagcttgc tcaaccaaac ttgggaacca tccttgatca acgaccaggt
     2341 gtcattacta aaggctttac ccagttgccc ggggatgcta tttatcacat ctcagattta
     2401 gaagaggatg aagaggaggg tattactttt caggttcagc aacctcttga agtggaagag
     2461 aaactttcaa catccaagcc agtaacaggg atcttcctgc cacccattac ttcagcaggt
     2521 ggaccagtta cagttgcaac cgccagccca ggaaagtgcc tgtcgtgcac aaactcaaca
     2581 ttcactttca ccacctgtag aatattacat ccctctgaca tcactcaggt tacccccagc
     2641 tctgggttcc cttcattatc ctgtggaagt agcggtagca gttcatccaa cacggctgtg
     2701 aattctcctg ccttgtccta tagactcagc attggtgagt ccatcaccaa ccgacgagat
     2761 tccactacaa ccttcagtag caccatgagc ttggccaaac ttctacaaga gcgaggcatc
     2821 tctgccaaag tgtaccacag cccaatttca gagaaccccc tccagcctct ccctaaatcc
     2881 ctggctatcc cttccacacc accaaattca ccatctcact caccttgccc ttctccttta
     2941 ccctttgagc ctcgagtgca tctctctgaa aattttttgg cctctcgacc agctgagaca
     3001 ttcctccagg agatgtatgg cttgagacac tcccggaacc ctcctgatgt tggccagttg
     3061 aagatgaact tagtggacag gctgaagaga ctggggatag ccagagtggt caagaaccct
     3121 ggtgcccaag agaatggaag atgccaggag gcagaaattg gtcctcaaaa accagattct
     3181 gctgtttatt taaattcagg tagcagttta ttaggtggac taaggaggaa tcagagtctt
     3241 ccagtcataa tgggtagctt tgctgcccca gtttgcacat cctcacccaa aatgggtgtc
     3301 ctgaaggagg actgagtctc gagggggggc ccggtacctt aattaattaa ggtaccaggt
     3361 aagtgtaccc aattcgccct atagtgagtc gtattacaat tcactcgatc gcccttccca
     3421 acagttgcgc agcctgaatg gcgaatggag atccaatttt taagtgtata atgtgttaaa
     3481 ctactgattc taattgtttg tgtattttag attcacagtc ccaaggctca tttcaggccc
     3541 ctcagtcctc acagtctgtt catgatcata atcagccata ccacatttgt agaggtttta
     3601 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     3661 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3721 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3781 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3841 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3901 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3961 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4021 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4081 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4141 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4201 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     4261 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4321 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4381 gaatcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4441 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4501 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4561 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4621 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4681 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4741 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     4801 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4861 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4921 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     4981 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5041 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5101 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5161 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5221 ggaagccggt cttgtcgatc aggatgatct ggacgaagaa catcaggggc tcgcgccagc
     5281 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     5341 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5401 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5461 tgctgaagaa cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5521 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     5581 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     5641 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5701 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     5761 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     5821 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     5881 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     5941 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     6001 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     6061 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6121 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6181 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6241 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6301 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6361 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6421 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6481 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6541 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6601 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6661 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6721 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6781 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6841 ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc
     6901 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     6961 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     7021 tgttcccat
//
