LOCUS       sequence-447847-        7146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MYC-tagged human TRAK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Pekkurnaz G, Trinidad JC, Wang X, Kong D, Schwarz TL
  TITLE     Glucose regulates mitochondrial motility via Milton modification by 
            O-GlcNAc transferase.
  JOURNAL   Cell. 2014 Jul 3;158(1):54-68. doi: 10.1016/j.cell.2014.06.007.
  PUBMED    24995978
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.06"; journalName: "Cell"; date: "2014-07-3- 3";
            volume: "158"; issue: "1"; pages: "54-68"
COMMENT     Sequence Label: MYC-hTRAK1
FEATURES             Location/Qualifiers
     source          1..7146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        320..523
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     474..494
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        569..587
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      619..628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             628..657
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3596..3615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3597..3615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3889..4010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3926..3945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3980..3999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4017..4472)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4154..4175)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4366..4385
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4499..4603
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4605..4962
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4605..4796
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4628..4648)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4813..4948
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4997..5791
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4997..5791
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5051..5070)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5661..5680
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5979..5998)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6023..6070
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6399..6987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6888..6907
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
       61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      541 gatccgctag cgattacgcc aagctcgaaa ttaaccctca ctaaagggaa caaaagctgg
      601 agctccaccg cggtggcggc cgccatggag cagaaactca tctctgaaga ggatctggcc
      661 cgggcggatc caatggcatt ggtttttcaa ttcgggcagc ccgtcagggc tcagcctctg
      721 ccaggactct gccacggcaa gctcattcgg acaaacgcct gtgatgtgtg caacagcacc
      781 gatcttccgg aagtcgagat cattagcctg ctggaggagc agctgcccca ttataagtta
      841 agagccgaca ccatctacgg ttatgaccac gacgactggc tccatacacc tctcatttct
      901 ccagatgcca acattgacct cacaaccgag caaattgaag agacgttaaa atacttcctt
      961 ttatgtgctg aaagagttgg ccagatgact aagacatata atgacataga tgctgtcact
     1021 cggcttcttg aggagaaaga gcgggattta gaattggccg ctcgcatcgg ccagtcgttg
     1081 ttgaagaaga acaagaccct aaccgagagg aacgagctgc tggaggagca ggtggaacac
     1141 atcagggagg aggtgtctca gctccggcat gagctgtcca tgaaggatga gctgcttcag
     1201 ttctacacca gcgctgcgga ggagagtgag cccgagtccg tttgctcaac cccgttgaag
     1261 aggaatgagt cgtcctcctc agtccagaat tactttcatt tggattctct tcaaaagaag
     1321 ctgaaagacc ttgaagagga gaatgttgta cttcgatccg aggccagcca gctgaagaca
     1381 gagaccatca cctatgagga gaaggagcag cagctggtca atgactgcgt gaaggagctg
     1441 agggatgcca atgtccagat tgctagtatc tcagaggaac tggccaagaa gacggaagat
     1501 gctgcccgcc agcaagagga gatcacacac ctgctatcgc aaatagttga tttgcagaaa
     1561 aaggcaaaag cttgcgcagt ggaaaatgaa gaacttgtcc agcatctggg ggctgctaag
     1621 gatgcccagc ggcagctcac agccgagctg cgtgagctgg aggacaagta cgcagagtgc
     1681 atggagatgc tgcatgaggc gcaggaggag ctgaagaacc tccggaacaa aaccatgccc
     1741 aataccacgt ctcggcgcta ccactcgctg ggcctgtttc ccatggattc cttggcagca
     1801 gagattgagg gaacgatgcg caaggagctg cagttggaag aggccgagtc tccagacatc
     1861 actcaccaga agcgtgtctt tgagacagta agaaacatca accaggttgt caagcagaga
     1921 tctctgaccc cttctcccat gaacatcccc ggctccaacc agtcctcggc catgaactcc
     1981 ctcctgtcca gctgcgtcag caccccccgg tccagcttct acggcagcga cataggcaac
     2041 gtcgtcctcg acaacaagac caacagcatc attctggaaa cagaggcagc cgacctggga
     2101 aacgatgagc ggagtaagaa gccggggacg ccgggcaccc caggctccca cgacctggag
     2161 acggcgctga ggcggctgtc cctgcgccgg gagaactacc tctcggagag gaggttcttt
     2221 gaggaggagc aagagaggaa gctccaggag ctggcggaga agggcgagct gcgcagcggc
     2281 tccctcacac ccactgagag catcatgtcc ctgggcacgc actcccgctt ctccgagttc
     2341 accggcttct ctggcatgtc cttcagcagc cgctcctacc tgcctgagaa gctccagatc
     2401 gtgaagccgc tggaaggttc tgccacactt caccactggc agcagttggc ccaacctcac
     2461 cttgggggca tcctggaccc ccggcctggt gtggtcacca agggcttccg gacgctggat
     2521 gttgacctgg acgaagtgta ctgccttaac gactttgaag aagatgacac aggtgaccac
     2581 atttctctcc cacgcctagc tacctccact ccagttcagc acccagagac ctcagcgcac
     2641 catcctggga agtgcatgtc tcagaccaac tccaccttca ccttcaccac ctgtcgcatc
     2701 ctgcatcctt cagatgagct cactcgggtc acaccaagcc ttaactcagc cccaactcca
     2761 gcttgtggca gcaccagcca cttgaaatcc acgccggtgg ccacaccatg cactccacgg
     2821 agactgagcc tggctgagtc cttcactaac acccgtgagt ccacgaccac catgagcaca
     2881 tccctggggc tcgtgtggct gttgaaggag cggggcattt ctgctgccgt gtacgacccc
     2941 cagagctggg acagggccgg ccggggctcc ctcctgcact cctacacgcc caagatggct
     3001 gtgatcccct ctactccgcc gaactcgcct atgcagacac ccacatcctc cccaccctcc
     3061 tttgagttca agtgcacgag ccctccctac gacaatttcc tggcttccaa gccagccagc
     3121 tccatcctga gggaagtgag agaaaagaac gtccgcagca gcgagagcca gaccgacgtg
     3181 tccgtctcca acctcaacct cgtggacaaa gtcaggaggt ttggggtggc caaagtggtg
     3241 aactcagggc gagcccatgt ccccaccttg actgaggagc agggacccct cctctgtggg
     3301 cccccggggc cagcaccagc ccttgttccc agaggcctgg tacctgaggg cctgcccctc
     3361 agatgcccca ctgtcaccag tgccatcggt gggctgcagc tcaatagtgg catccggcgg
     3421 aatcgcagct tccccaccat ggtgggatct agcatgcaga tgaaagctcc tgtgactctc
     3481 acctcgggca tcttgatggg tgctaagctc tccaaacaaa ctagcttacg gtaagtctcg
     3541 agggggggcc cggtacctta attaattaag gtaccaggta agtgtaccca attcgcccta
     3601 tagtgagtcg tattacaatt cactcgatcg cccttcccaa cagttgcgca gcctgaatgg
     3661 cgaatggaga tccaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     3721 gtattttaga ttcacagtcc caaggctcat ttcaggcccc tcagtcctca cagtctgttc
     3781 atgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     3841 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     3901 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3961 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat
     4021 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     4081 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     4141 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     4201 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     4261 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     4321 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     4381 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     4441 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     4501 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4561 ataaccctga taaatgcttc aataatattg aaaaaggaag aatcctgagg cggaaagaac
     4621 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     4681 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     4741 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     4801 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4861 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     4921 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     4981 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     5041 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5101 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5161 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5221 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5281 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5341 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5401 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5461 ggatgatctg gacgaagaac atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5521 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5581 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5641 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagaac ttggcggcga
     5701 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5761 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     5821 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     5881 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5941 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     6001 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     6061 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     6121 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     6181 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     6241 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6301 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6361 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6421 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6481 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6541 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6601 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6661 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6721 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6781 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6841 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6901 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6961 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7021 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7081 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
     7141 tagccc
//
