LOCUS       sequence-44785-e        1218 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Dieriks B, Van Oostveldt P
  TITLE     Spatiotemporal behavior of nuclear cyclophilin B indicates a role in
            RNA transcription.
  JOURNAL   Int J Mol Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937.
  PUBMED    22426501
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937."
COMMENT     Sequence Label: PcDNA-DEST53-CypB-Nucl2 N-term GFP
FEATURES             Location/Qualifiers
     source          1..1218
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(546..645)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(662..681)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(663..681)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(686..702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(686..702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(803..822)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(881..900)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 actatgtggg gatgagagag aggggcccaa agtcaccgtc aaggtgtatt ttgacctacg
       61 aattggagat gaagatgtag gccgggtgat ctttggtctc ttcggaaaga ctgttccaaa
      121 aacagtggat aattttgtgg ccttagctac aggagagaaa ggatttggct acaaaaacag
      181 caaattccat cgtgtaatca aggacttcat gatccagggc ggagacttca ccaggggaga
      241 tggcacagga ggaaagagca tctacggtga gcgcttcccc gatgagaact tcaaactgaa
      301 gcactacggg cctggctggg tgagcatggc caacgcaggc aaagacacca acggctccca
      361 gttcttcatc acgacagtca agacagcctg gctagatggc aagcatgtgg tgtttggcaa
      421 agttctagag ggcatggagg tggtgcggaa ggtggagagc accaagacag acagccggga
      481 taaacccctg aaggatgtga tcatcgcaga ctgcggcaag atcgaggtgg agaagccctt
      541 ttagaaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
      601 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
      661 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
      721 tcaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
      781 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
      841 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
      901 aatgtcgggc aatcaggtgc gaccatctat cgcttgtatg ggaaagcccg atgcgccaga
      961 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttaacagaa tgagatggtc
     1021 agactaactg gctgacgtac ttatgccttc tccgacatca gcattttatc cgtactctgg
     1081 atggatgcat ggtaactcac cactgccgat cccgtaaaca gcatcaggat aaagaaattc
     1141 ctgattcaag tggaaaatgt tgatgccttg caggttcctg cccgtgcatt cgatcctgtt
     1201 tgtaatgtca tttacacg
//
