LOCUS       sequence-447857-       11584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Start codon reporter with mutated start codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   She R, Luo J, Weissman JS
  TITLE     Translational fidelity screens in mammalian cells reveal eIF3 and 
            eIF4G2 as regulators of start codon selectivity.
  JOURNAL   Nucleic Acids Res. 2023 Jul 7;51(12):6355-6369. doi: 
            10.1093/nar/gkad329.
  PUBMED    37144468
REFERENCE   2  (bases 1 to 11584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-07-7- 7"; volume: "51"; issue: 
            "12"; pages: "6355-6369"
COMMENT     Sequence Label: pGFP-AUC-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..11584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          20..85
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             215..235
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    660..794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(697..716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     751..770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             962..1595
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1642..1767
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2264..2497
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2682..2726
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3011..3128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3870..4277
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             4318..5031
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             4318..5028
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(4345..4373)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4968..4988
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    join(5044..5617,5618..5620,5621..5629)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5210..5227)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5437..5456
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5642..6352
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5787..5805)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6055..6074
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6409..6997
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6462..6482)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6880..6891)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7000..7016)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7001..7017)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7107..7340
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7365..7383)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7366..7383)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7673..7692)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7906..7925)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8025..8047
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8085..8103)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8171..8275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8276..8344,8345..9136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8494..8513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9307..9895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9796..9815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10049..10066
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10136..10156)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10141..10470
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10321..10456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
       61 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
      121 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
      181 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
      241 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
      301 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
      361 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
      421 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
      481 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
      541 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      601 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      661 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      721 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      781 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
      841 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
      901 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
      961 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
     1021 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     1081 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     1141 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     1201 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     1261 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     1321 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     1381 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     1441 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     1501 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     1561 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     1621 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     1681 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     1741 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     1801 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     1861 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1921 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1981 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     2041 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     2101 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     2161 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     2221 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     2281 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     2341 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     2401 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     2461 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     2521 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     2581 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     2641 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     2701 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     2761 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2821 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2881 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     2941 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa atggcagtat
     3001 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3061 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3121 aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat aagcttgata
     3181 tcgaattcgg gaggtggtcc ctgcagttac gccaatgata ccccccgcca gaaaaatctt
     3241 agtagccttc cctttttgtt ttccgtgccc caactcggcg gattgactcg gccccttccg
     3301 gaaacacccg aatcaacttc tagtcaaatt attgttcacg ccgcaatgac ccacccctgg
     3361 cccgcgtctg tggaactgac ccctggtgta caggagagtt cgctgctgaa agtggtccca
     3421 aaggggtact agtttttaag ctcccaactc cccctccccc agcgtctgga ggattccaca
     3481 ccctcgcacc gcaggggcga ggaagtgggc ggagtccggt tttggcgcca gccgctgagg
     3541 ctgccaagca gaaaagccac cgctgaggag actccggtca ctgtcctcgc cccgcctccc
     3601 ccttccctcc ccttggggac caccgggcgc cacgccgcga acggtaagtg ccgcggtcgt
     3661 cggcgcctcc gccctccccc tagggcccca attcccagcg ggcgcggcgc gcggccccgc
     3721 gcgcagctcc cggctccctc ccccttcgga tgtggcttga gctgtaggcg cggagtcctg
     3781 cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     3841 ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca tggaaaaata
     3901 ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag ctaacgttgg
     3961 gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa gaacagatgg
     4021 tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga tatggcccaa
     4081 ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg acctgaaatg
     4141 accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt cgcgcgcttc
     4201 tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca gtcctccgac
     4261 agactgagtc gcccgggggg gatctggagc tctcgagaat tctcagccgc caccatcagc
     4321 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     4381 aatgggcaca aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc
     4441 acccttaaat ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact
     4501 actctgacct atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac
     4561 tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat
     4621 gacgggacct acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt
     4681 atcgagttaa agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgag
     4741 tacaacttta actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa
     4801 gctaacttca aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat
     4861 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg
     4921 acacaatctg tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag
     4981 tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaata ataagcggcc
     5041 gcgcccctct ccctcccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5101 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     5161 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5221 ggaatgcaag gtctgttgaa tgtcgttaag gaagcagttc ctctggaagc ttcttgaaga
     5281 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5341 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5401 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5461 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5521 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5581 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggcgtccgg
     5641 aatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     5701 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     5761 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     5821 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     5881 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     5941 agtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     6001 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     6061 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     6121 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     6181 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     6241 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     6301 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aataagcgac
     6361 tctagagtcg acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg
     6421 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6481 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6541 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6601 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6661 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6721 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6781 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     6841 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6901 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6961 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggg
     7021 aattaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7081 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7141 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7201 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7261 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7321 agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta
     7381 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
     7441 atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg
     7501 taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc
     7561 ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac
     7621 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     7681 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     7741 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     7801 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     7861 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga
     7921 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     7981 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     8041 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     8101 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     8161 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8221 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8281 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8341 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8401 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8461 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8521 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8581 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8641 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8701 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8761 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8821 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8881 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8941 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9001 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9061 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9121 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9181 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9241 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9301 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9361 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9421 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     9481 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9541 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9601 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9661 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9721 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9781 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9841 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9901 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9961 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    10021 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    10081 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat
    10141 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10201 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
    10261 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
    10321 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    10381 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    10441 ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat
    10501 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
    10561 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
    10621 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
    10681 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
    10741 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg
    10801 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
    10861 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
    10921 atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat
    10981 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac
    11041 tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta
    11101 ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
    11161 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
    11221 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
    11281 gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg
    11341 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
    11401 tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac
    11461 cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg
    11521 ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
    11581 ctac
//
