LOCUS       sequence-447862-        9914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EIF3D variant overexpression vector with sgRNA targeting eIF3D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   She R, Luo J, Weissman JS
  TITLE     Translational fidelity screens in mammalian cells reveal eIF3 and 
            eIF4G2 as regulators of start codon selectivity.
  JOURNAL   Nucleic Acids Res. 2023 Jul 7;51(12):6355-6369. doi: 
            10.1093/nar/gkad329.
  PUBMED    37144468
REFERENCE   2  (bases 1 to 9914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-07-7- 7"; volume: "51"; issue: 
            "12"; pages: "6355-6369"
COMMENT     Sequence Label: pEIF3D-S528N-S529N-sgEIF3D
FEATURES             Location/Qualifiers
     source          1..9914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2814..2847)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2851..2871
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        3195..3602
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3614..4210
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3614..3633)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4110..4130
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4220..4273
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4286..4981
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             4991..5047
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(6714..6747)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6803..7391
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6856..6876)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7274..7285)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7394..7410)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7395..7411)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7920..8100
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8162..8750)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8242..8261)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8921..9712,9713..9781))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9544..9563
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9782..9886)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag atccgacgcg ccatctctag
     2641 gcccgcgccg gccccctcgc acggacttgt gggagaagct cggctactcc cctgccccgg
     2701 ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga
     2761 taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt tctataactt
     2821 cgtatagcat acattatacg aagttataaa cagcacaaaa ggaaactcac cctaactgta
     2881 aagtaattgt gtgttttgag actataagta tcccttggag aaccaccttg ttggctgctg
     2941 ctaacgcggt cccgtttaag agctaagctg gaaacagcat agcaagttta aataaggcta
     3001 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctcg agtactagga
     3061 tccattaggc ggccgcgtgg ataaccgtat taccgccatg cattgcagcc ccgataaaat
     3121 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     3181 gcaagctagc tgcagtaacg ccattttgca aggcatggaa aaataccaaa ccaagaatag
     3241 agaagttcag atcaagggcg ggtacatgaa aatagctaac gttgggccaa acaggatatc
     3301 tgcggtgagc agtttcggcc ccggcccggg gccaagaaca gatggtcacc gcagtttcgg
     3361 cccgggcccg aggccaagaa cagatggtcc ccagatatgg cccaaccctc agcagtttct
     3421 taagacccat cagatgtttc caggctcccc caaggacctg aaatgaccct gcgccttatt
     3481 tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc gcttctgctt cccgagctct
     3541 ataaaagagc tcacaacccc tcactcggcg cgccagtcct ccgacagact gagtcgcccg
     3601 gggctagccc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     3661 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     3721 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     3781 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     3841 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     3901 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     3961 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     4021 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4081 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4141 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     4201 gcccggtgcc ggatcgggag agggcagagg aagtctgcta acatgcggtg acgtcgagga
     4261 gaatcctggc ccaccggtcg ccaccagcga gctgattaag gagaacatgc acatgaagct
     4321 gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa
     4381 gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt
     4441 cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac
     4501 ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt
     4561 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
     4621 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat
     4681 gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg
     4741 cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa
     4801 catcaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta
     4861 ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca
     4921 gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa
     4981 tggtagtggg gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc
     5041 tggacctgca aagttcatga cacccgtgat ccaggacaac ccctcaggct ggggtccctg
     5101 tgcggttccc gagcagtttc gggatatgcc ctaccagccg ttcagcaaag gagatcggct
     5161 aggaaaggtt gcagactgga caggagccac ataccaagat aagaggtaca caaataagta
     5221 ctcctctcag tttggtggtg gaagtcaata tgcttatttc catgaggagg atgaaagtag
     5281 cttccagctg gtggatacag cgcgcacaca gaagacggcc taccagcgga atcgaatgag
     5341 atttgcccag aggaacctcc gcagagacaa agatcgtcgg aacatgttgc agttcaacct
     5401 gcagatcctg cctaagagtg ccaaacagaa agagagagaa cgcattcgac tgcagaaaaa
     5461 gttccagaaa caatttgggg ttaggcagaa atgggatcag aaatcacaga aaccccgaga
     5521 ctcttcagtt gaagttcgta gtgattggga agtgaaagag gaaatggatt ttcctcagtt
     5581 gatgaagatg cgctacttgg aagtatcaga gccacaggac attgagtgtt gtggggccct
     5641 agaatactac gacaaagcct ttgaccgcat caccacgagg agtgagaagc cactgcggag
     5701 catcaagcgc atcttccaca ctgtcaccac cacagacgac cctgtcatcc gcaagctggc
     5761 aaaaactcag gggaatgtgt ttgccactga tgccatcctg gccacgctga tgagctgtac
     5821 ccgctcagtg tattcctggg atattgtcgt ccagagagtt gggtccaaac tcttctttga
     5881 caagagagac aactctgact ttgacctcct gacagtgagt gagactgcca atgagccccc
     5941 tcaagatgaa ggtaattcct tcaattcacc ccgcaacctg gccatggagg caacctacat
     6001 caaccacaat ttctcccagc agtgcttgag aatggggaag gaaagataca acttccccaa
     6061 cccaaacccg tttgtggagg acgacatgga taagaatgaa atcgcctctg ttgcgtaccg
     6121 ttaccgcagg tggaagcttg gagatgatat tgaccttatt gtccgttgtg agcacgatgg
     6181 cgtcatgact ggagccaacg gggaagtgtc cttcatcaac atcaagacac tcaatgagtg
     6241 ggattccagg cactgtaatg gcgttgactg gcgtcagaag ctggactctc agcgaggggc
     6301 tgtcattgcc acggagctga agaacaacag ctacaagttg gcccggtgga cctgctgtgc
     6361 tttgctggct ggatctgagt acctcaagct tggttatgtg tctcggtacc acgtgaaaga
     6421 ctcctcacgc cacgtcatcc taggcaccca gcagttcaag cctaatgagt ttgccagcca
     6481 gatcaacctg agcgtggaga atgcctgggg cattttacgc tgcgtcattg acatctgcat
     6541 gaagctggag gagggcaaat acctcatcct caaggacccc aacaagcagg tcatccgtgt
     6601 ctacagcctc cctgatggca ccttcaacaa cgatgaagat gaggaggaag aggaggagga
     6661 agaagaggaa gaagaagagg aagaaactta ataagaattc gtcgagggac ctaataactt
     6721 cgtatagcat acattatacg aagttataca tgtttaaggg ttccggttcc actaggtaca
     6781 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     6841 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6901 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6961 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7021 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7081 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7141 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7201 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7261 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7321 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7381 cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat ggagcaatca
     7441 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7501 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7561 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7621 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7681 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     7741 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     7801 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     7861 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     7921 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7981 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8041 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8101 gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8161 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8221 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8341 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8401 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8461 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8521 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8581 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8641 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8701 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8761 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8821 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8881 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8941 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9001 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9061 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9121 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9181 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9241 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9301 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9361 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9421 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9481 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9541 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9601 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9661 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9721 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9781 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9841 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9901 aagtgccacc tgac
//
