LOCUS       sequence-447904-        2523 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in BL21(DE3) of T4 primase and helicase fusion with 24 
            amino acid linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Manosas M, Spiering MM, Zhuang Z, Benkovic SJ, Croquette V
  TITLE     Coupling DNA unwinding activity with primer synthesis in the 
            bacteriophage T4 primosome.
  JOURNAL   Nat Chem Biol. 2009 Dec;5(12):904-12. doi: 10.1038/nchembio.236. 
            Epub 2009 Oct 18.
  PUBMED    19838204
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.236"; journalName: "Nat Chem Biol"; date: 
            "2009-12"; volume: "5"; issue: "12"; pages: "904-12"
COMMENT     Sequence Label: pTBY1-T4gp61/41 fusion
FEATURES             Location/Qualifiers
     source          1..2523
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtcatcaa taccttggat tgataatgag tttgcatacc gtgcattagc tcatttacct
       61 aaattcacac aagtaaataa tagttcaact tttaaattac ggtttagatg ccctgtttgt
      121 ggagattcaa aaaccgacca gaataaagcc cgtggatggt attatggtga taataatgaa
      181 ggaaatattc attgttataa ctgtaactat catgcaccaa tcggaatata tttaaaggag
      241 tttgaacccg atttatatcg tgagtatatc tttgaaataa gaaaagaaaa aggtaaaagt
      301 cgtccaatag aaaaacctaa agaacttcct aaacaacccg agaagaaaat aattaaatct
      361 cttccgtcat gtgttagatt agataaactg gcggaagacc atccaattat aaaatacgta
      421 aaagctcgtt gtattccaaa ggataaatgg aaatatcttt ggtttacaac cgaatggcct
      481 aaattagtta atagcatagc accgggaaca tacaaaaagg aaatttctga gcctcgtctt
      541 gttattccaa tttataatgc taatggaaaa gctgagtctt ttcaaggacg tgcattaaag
      601 aaagatgctc ctcaaaaata tatcaccatc aaagcttatc ctgaggcaac aaaaatctat
      661 ggagtcgaac gagttaaaga tggtgatgta tatgttctag aaggacctat agattcactt
      721 tttattgaaa atggtatagc tattacgggc ggtcaattag acctagaagt tgttccattt
      781 aaagatagac gtgtgtgggt cttagataat gaacctcgtc accctgacac tattaaacga
      841 atgactaaat tagttgatgc aggagaaagg gttatgtttt gggataaatc tccctggaaa
      901 tcaaaagatg ttaatgatat gattagaaag gaaggtgcaa cccctgaaca aattatggaa
      961 tatatgaaaa ataatattgc ccaggggtta atggctaaaa tgcggctatc taaatatgct
     1021 aagggtggtg gtggtagcga aggtggtggt agcggtgaag gtggtggtac cggtggtggt
     1081 agcgaaggtg gtggtagcat ggtagaaatt attctttctc atctcatatt tgatcaagct
     1141 tatttttcaa aagtttggcc atatatggat tcagaatatt ttgaaagtgg tccagctaaa
     1201 aatacattca aattaattaa atctcatgtt aatgagtacc atagcgttcc atctattaat
     1261 gcgttaaatg ttgcattaga aaatagttca tttactgaaa cagaatattc tggtgtaaaa
     1321 acacttattt caaaactggc tgattctccg gaagaccaca gctggttagt aaaagaaaca
     1381 gaaaaatatg ttcagcaaag ggcgatgttt aatgctacgt ctaaaataat cgaaattcaa
     1441 actaatgctg agcttcctcc ggaaaaacga aataagaaaa tgccggatgt tggtgctatt
     1501 cctgacatca tgcgccaggc attatcaatt tcatttgata gttacgttgg tcatgattgg
     1561 atggatgact acgaagcacg ttggctatct tatatgaata aagctcgtaa ggttccattt
     1621 aaactcagaa ttctgaacaa aattacgaaa ggcggagctg aaactggaac actgaacgtt
     1681 ttaatggctg gcgttaacgt tggtaaatca ttaggattgt gttcattagc ggcagattat
     1741 ttacagctcg gacataatgt tctttacatt tccatggaaa tggcagaaga agtctgtgct
     1801 aaacgtattg acgctaatat gcttgatgtt tctcttgatg acattgatga tgggcatatt
     1861 tcttacgctg agtataaagg aaaaatggaa aaatggcgtg agaaatctac tcttggtcgt
     1921 ttaatcgtta aacagtatcc tactggtgga gcagacgcta atacatttcg atcactttta
     1981 aacgaattga agctcaagaa gaattttgtt ccaacaatca ttattgtcga ctatctaggt
     2041 atttgtaaat cttgccgcat tagagtttat tcagaaaata gttacacaac tgttaaagct
     2101 attgcagagg aattacgtgc tctggctgtt gaaaccgaaa ctgttctttg gactgcagca
     2161 caggttggta aacaagcttg ggactcttcc gatgttaaca tgagcgatat tgcagaatct
     2221 gccggtcttc cagcaacagc cgattttatg cttgcggtca ttgaaaccga ggagctagca
     2281 gctgctgaac aacaactcat taagcaaatc aaatcacgat atggtgataa gaataagtgg
     2341 aataagtttt tgatgggtgt tcaaaaagga aatcaaaaat gggtagaaat tgaacaagat
     2401 tctactccaa ctgaagtgaa cgaagtagca ggttcacaac agattcaggc tgagcagaat
     2461 cgctatcaaa gaaatgaatc gactcgagct cagttagatg ctttggcgaa tgaattaaaa
     2521 ttt
//
