LOCUS       sequence-447918-        8684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With
            Blasticidin Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pLentiPGK Blast DEST JNKKTRmRuby2
FEATURES             Location/Qualifiers
     source          1..8684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1738..2248
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2167..2185
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    2303..2327
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2356..2408
                     /label=MCS
                     /note="multiple cloning site"
     CDS             2615..3322
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     protein_bind    complement(3349..3373)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3404..3992
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3457..3477)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3875..3886)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4043..4063)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4048..4377
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4228..4363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4425..4472
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4491..4889
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4976..5209
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5281..5402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5318..5337)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5372..5391
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5442..5577
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5597..5616)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5598..5616)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5626..5643)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5626..5642)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5635..5657)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5784..6239
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5871..5890)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6081..6102
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6265..6369
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6370..6438,6439..7230)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6588..6607)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7401..7989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7890..7909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8143..8160
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8277..8298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8313..8318,8319..8336,8337..8343)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8351..8367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8356..8378
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8375..8391
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8375..8391
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8410..8430
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8412..8430
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8458..8684
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 attcaaaatt ttatcgatca cgagactagc ctcgagaagc ttgatatcga attccacggg
     1741 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     1801 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     1861 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     1921 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     1981 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     2041 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     2101 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     2161 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     2221 tgaccgaatc accgacctct ctccccaggg ggatccaccg gttcgtcgac tagtccagtg
     2281 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttta aaggaaccaa
     2341 ttcagtcgac tggatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
     2401 cgggatccac cggccggtcg ccaccatgag taaccctaag atcctaaaac agagcatgac
     2461 cttgaacctg gccgacccgg tgggcagtct gaagccgcac ctccgcgcca agcgctcggc
     2521 cggtctcaac gaagacgagt cgccatctaa gaagctggcg tcgccggagg tgtcgtcaag
     2581 gctggagcgc ctgaccctcc agtccagcac tagtgtgtct aagggcgaag agctgatcaa
     2641 ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc aacggccacc aattcaaatg
     2701 cacaggtgaa ggagaaggca atccgtacat gggaactcaa accatgagga tcaaagtcat
     2761 cgagggagga cccctgccat ttgcctttga cattcttgcc acgtcgttca tgtatggcag
     2821 ccgtactttt atcaagtacc cgaaaggcat tcctgatttc tttaaacagt cctttcctga
     2881 gggttttact tgggaaagag ttacgagata cgaagatggt ggagtcgtca ccgtcatgca
     2941 ggacaccagc cttgaggatg gctgtctcgt ttaccacgtc caagtcagag gggtaaactt
     3001 tccctccaat ggtcccgtga tgcagaagaa gaccaagggt tgggagccta atacagagat
     3061 gatgtatcca gcagatggtg gtctgagggg atacactcat atggcactga aagttgatgg
     3121 tggtggccat ctgtcttgct ctttcgtaac aacttacagg tcaaaaaaga ccgtcgggaa
     3181 catcaagatg cccggtatcc atgccgttga tcaccgcctg gaaaggttag aggaaagtga
     3241 caatgaaatg ttcgtagtac aacgcgaaca cgcagttgcc aagttcgccg ggcttggtgg
     3301 tgggatggac gagctgtaca agtaagcggc cgcactcgag atatctagac ccagctttct
     3361 tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gacaatcaac ctctggatta
     3421 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3481 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3541 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3601 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3661 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3721 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3781 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     3841 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3901 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3961 gagtcggatc tccctttggg ccgcctcccc gcctggaatt ctgcagatat ccggttagta
     4021 atgagtttgg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4081 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4141 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4201 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4261 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     4321 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4381 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     4441 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagc
     4501 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     4561 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     4621 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     4681 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     4741 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
     4801 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     4861 tgccctctgg ttatgtgtgg gagggctaag cacaattcga gctcggtacc tttaagacca
     4921 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     4981 gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     5041 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5101 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5161 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     5221 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     5281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5401 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     5461 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5521 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5581 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     5641 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     5701 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     5761 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5821 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5881 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5941 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6001 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6061 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6121 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6181 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     6241 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6301 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6361 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6421 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6481 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6541 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6601 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6661 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6721 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6781 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6841 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6901 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6961 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7021 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7081 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7141 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7201 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7261 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7321 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7381 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7441 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7501 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7561 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7621 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7681 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7741 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7801 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7861 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7921 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7981 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8041 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8101 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8161 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     8221 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     8281 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     8341 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     8401 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     8461 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     8521 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     8581 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     8641 attgcagaga tattgtattt aagtgcctag ctcgatacat aaac
//
