LOCUS       sequence-447938-        6840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF804B transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF804B
FEATURES             Location/Qualifiers
     source          1..6840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4151..4250)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4267..4286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4268..4286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4291..4307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4291..4307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4738..5529
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5622..6210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6111..6130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6364..6381
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6540..6567)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6659..6745)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6794..6816
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6808..6825
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6809..6825
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccatggcttg ttacctggtc
      121 atcagttcga gacatctcag caatgggcac taccggggca ttaaaggagt cttcagggga
      181 cccctgtgca agaacggatc tccctctccg gattttgcag aaaagaagtc cacagcaaag
      241 gccctggaag atgtaaaggc aaacttttac tgtgaattat gtgacaagca gtatcacaaa
      301 caccaggagt ttgacaatca tattaattct tatgaccatg ctcataagca gagactgaaa
      361 gaattaaagc aacgggaatt tgctcgaaat gtagcttcta agtcatggaa agatgagaaa
      421 aaacaagaaa aagcacttaa acgacttcat cagctggctg agttaaggca gcaatctgaa
      481 tgtgtttctg gaaatggacc agcatacaaa gcccccaggg tagccataga aaagcaactc
      541 cagcaaggaa ttttccccat taagaatggc agaaaggtat catgcatgaa gagtgctctt
      601 ctccttaaag gaaaaaatct ccccagaatc atatccgata aacagcggtc caccatgcca
      661 aatcgacacc aattacaatc agacaggcgt tgtttgtttg gaaatcaggt actgcaaaca
      721 tcttcagatc tcagcaatgc aaatcacaga acaggagtat catttacttt ttccaaaaaa
      781 gtgcacctaa aattagaatc ttcagcatca gttttcagtg agaacacaga agaaacccat
      841 gattgtaaca agtcacccat ttataaaaca aaacaaactg cagataagtg caagtgctgc
      901 aggtttgcaa ataaagatac acaccttacc aaggaaaaag aggtaaatat ctcaccaagc
      961 catctggaaa gtgttttaca caataccatc tccataaact ctaaaatttt gcaagacaaa
     1021 cacgactcta ttgatgagac actagaagat tcaattggca ttcatgcttc attctctaaa
     1081 tctaacattc atctttcaga tgtagatttt actcctacca gcagagaaaa agaaactaga
     1141 aatacattga agaacacttt agaaaattgt gttaatcacc catgccaagc aaatgcttcc
     1201 ttcagcccac caaacattta caaccatagt gatgccagga tatctgaatg cctggatgag
     1261 ttttcatcac tggagccaag tgaacaaaag agtacagtgc atctgaatcc aaattccaga
     1321 atagagaaca gagaaaaatc tttagataaa acagaaagag ttagcaaaaa tgttcaaaga
     1381 cttgtaaaag aagcatgtac ccataatgtg gcatctaaac cactaccttt tctccacgtt
     1441 caaagcaagg atggccacac cactcttcaa tggcctacgg aacttctgct ctttacaaaa
     1501 acagaaccct gtatctctta tggctgcaac ccactgtatt ttgattttaa gctttctcgg
     1561 aacacaaagg aagaccacaa tctagaggac ttaaaaacag aattgggtaa gaagcccttg
     1621 gaattgaaga ctaaaagaga gagccaagtc tcaggtttaa ctgaagacca acaaaaattg
     1681 atccaagaag attatcaata tccgaaacca aagacgatga tagctaatcc ggattgggaa
     1741 aaattccaga ggaaatataa tttggactac agtgattctg agccaaataa gagtgaatat
     1801 actttcagtg caaatgattt ggaaatgaaa aatcctaaag tgcctcttta cctcaacaca
     1861 tctctaaagg attgtgctgg aaagaataat agtagtgaga acaaacttaa ggaagcttca
     1921 agggcccatt ggcaaggctg cagaaaggca gttctaaatg atatagatga ggacctatct
     1981 tttccttcct acatctctag gtttaaaaag cataaattga ttccctgcag tcctcatttg
     2041 gaatttgaag atgaaagaca attcaactgc aagtccagtc cttgtacagt agggggtcac
     2101 agtgaccatg ggaaagactt cagtgtaatt ttgaagagta accacatcag catgaccagc
     2161 aaggtttccg gatgtggaaa ccaaagatac aagagatact ctccacagtc atgtttgagt
     2221 agatattctt cctctttgga cacatcccct agcagcatgt ctagcttgag aagtacttgt
     2281 tcaagtcata gattcaatgg taatagcaga ggtaatttgc tctgcttcca taaaagagaa
     2341 caccactcag ttgaaaggca caaacggaaa tgtctaaagc acaactgctt ctacttgtct
     2401 gatgatataa caaagagcag ccaaatgcag tctgaaccac agaaagagag gaactgcaaa
     2461 ttgtgggaat catttaaaaa tgaaaaatac tcaaaacgta gatattgtca ctgcagagaa
     2521 agacaaaaac tgggcaaaaa tcaacaacaa ttttcagggc taaaatctac gagaatcatc
     2581 tattgtgatt ctaactcaca gatttcctgt actggaagca gtaaaaaacc acctaattgc
     2641 cagggaactc agcacgacag attggactct tactcaatag agaaaatgta ttacttgaat
     2701 aaaagcaaga gaaatcaaga gtctttgggc agccctcaca tttgtgatct gggaaaagtc
     2761 aggcccatga agtgtaactc cgggaatatc agctgccttc taaagaactg ttccagtggc
     2821 ccttcagaaa ccacagaatc aaacactgca gaaggagaga ggacccctct aacagcaaaa
     2881 atccttttag aaagagtaca agccaagaaa tgtcaagaac aatcaagtaa tgttgagatc
     2941 tcttcaaaca gttgtaaaag tgaattagag gctccttcgc aagtcccatg cacaattcaa
     3001 cttgcaccat caggctgtaa cagacaagca ttgcctttgt ctgaaaaaat acagtatgca
     3061 agtgagagca gaaatgatca agacagtgca attccaagga ctacggagaa agacaaaagc
     3121 aaaagttcac acacaaataa ttttacaatt ttagcagaca ctgattgtga taaccatctt
     3181 tctaaaggta taattcacct agtaacagag tctcagtcac taaacataaa aagggatgca
     3241 acaacaaaag aacaatcaaa acctttaatt agtgaaatcc aaccttttat tcaaagctgt
     3301 gacccagtac caaatgaatt ccctggtgct tttccgtcta ataaatatac tggtgtgact
     3361 gattcaacag agacccaaga agaccaaata aatctagact tacaggatgt aagcatgcat
     3421 ataaatcatg tagagggaaa tataaactct tactatgaca gaactatgca gaaacctgac
     3481 aaagtcgaag acggattaga aatgtgtcat aaatctatct ctcccccttt aattcaacag
     3541 cccataacat tttctcctga cgaaatagat aaatataaga tcctacagct acaagcccag
     3601 cagcatatgc agaagcaact cctatcaaag catcttcgag ttttgcctgc tgcagggcct
     3661 actgccttct ctccggcctc aaccgtacag acagttccag ttcaccagca cacttctatc
     3721 accaccatcc accacacgtt cctgcagcat tttgctgttt ctgcttcctt aagttctcat
     3781 agcagtcacc tccctattgc tcatctacat cctctttcac aggcacattt cagtcctatt
     3841 tcattttcga ctctgactcc aaccattatc cctgcacacc ccactttctt agcaggtcat
     3901 cccctgcatt tagtagctgc tacccccttc cacccatctc acataacact tcagcctctg
     3961 ccccctacag catttattcc tacattgttt ggtcctcact taaatccagc cacaacttct
     4021 atcatccact tgaatccttt aatccaacca gtattccaag gtcaagattt ttgccatcat
     4081 tcttgctcta gccagatgca acagctaaat gaagtgaaag aggccttaaa tgtgtccaca
     4141 cacttgaact tgccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     4201 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     4261 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     4321 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
     4381 tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
     4441 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     4501 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     4561 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     4621 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     4681 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
     4741 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     4801 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     4861 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     4921 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     4981 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     5041 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     5101 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     5161 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     5221 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     5281 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     5341 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     5401 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     5461 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     5521 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     5581 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5641 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5701 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5761 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5821 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5881 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5941 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6001 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6061 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6121 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6181 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6241 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6301 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6361 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6421 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6481 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     6541 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     6601 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     6661 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     6721 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     6781 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
