LOCUS       sequence-447979-         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for yeast gene knockout, uses auxotrophic marker 
            TRP1 (Kluyveromyces lactis).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Feng Y-H, Song J-Z, Zhu J, Xie Z
  TITLE     Template plasmids optimized for deletion of multiple genes in yeast 
            Saccharomyces cerevisiae.
  JOURNAL   Microbiol Spectr. 2024 Oct 10:e0132024. doi: 
            10.1128/spectrum.01320-24.
  PUBMED    39387598
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr. 2024 Oct 10:e0132024. doi: 10.1128/spectrum.01320-24."
COMMENT     Sequence Label: SJZ2-TRP1
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctcgtta aagtgtgtgg tttgcaaacc gttgaagctg caaagactgc tgtggatgat
       61 ggtgctgatt acttaggtat catttgtgtt cccggtagga aaagaaccat tgactcatct
      121 gttgcgaaag gtatttcaac tgcagttcac caacaagaga acgtgaaagg tactaaacta
      181 gtcggggtgt ttagaaatca gtccgttgat gatgtccttc aactgtacca cgaatataat
      241 ctagatgtga tacaattaca tggagatgaa gatattaaag aatacagatc tttgatccca
      301 tcttcaattc caatcattaa gaggttccag ttcccacagg attgtgaatt actactggac
      361 ctgtatgaac acgtagacaa tgtgctgacg ttgttcgatt ctggtgaagg tggcactggt
      421 gagaaattga attggagtgc aatttccagt tggtctgcaa gtcatcccga gataaaattc
      481 attatcgctg gtggattgaa tcctgataac gtttctgttg ccattaatat gttaccaaat
      541 gcgatcggtg tcgatgtaag tggaggagta gagactgatg gtatcaagga tttagaaaag
      601 gtaaagctat tcatccagca ggcctctcaa tag
//
