LOCUS       sequence-447981-         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for yeast gene knockout, confers antibiotic 
            resistance to nourseothricin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Feng Y-H, Song J-Z, Zhu J, Xie Z
  TITLE     Template plasmids optimized for deletion of multiple genes in yeast 
            Saccharomyces cerevisiae.
  JOURNAL   Microbiol Spectr. 2024 Oct 10:e0132024. doi: 
            10.1128/spectrum.01320-24.
  PUBMED    39387598
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr. 2024 Oct 10:e0132024. doi: 10.1128/spectrum.01320-24."
COMMENT     Sequence Label: SJZ4-NAT
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..573
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 atgggtacca ctcttgacga cacggcttac cggtaccgca ccagtgtccc gggggacgcc
       61 gaggccatcg aggcactgga tgggtccttc accaccgaca ccgtcttccg cgtcaccgcc
      121 accggggacg gcttcaccct gcgggaggtg ccggtggacc cgcccctgac caaggtgttc
      181 cccgacgacg aatcggacga cgaatcggac gacggggagg acggcgaccc ggactcccgg
      241 acgttcgtcg cgtacgggga cgacggcgac ctggcgggct tcgtggtcgt ctcgtactcc
      301 ggctggaacc gccggctgac cgtcgaggac atcgaggtcg ccccggagca ccgggggcac
      361 ggggtcgggc gcgcgttgat ggggctcgcg acggagttcg cccgcgagcg gggcgccggg
      421 cacctctggc tggaggtcac caacgtcaac gcaccggcga tccacgcgta ccggcggatg
      481 gggttcaccc tctgcggcct ggacaccgcc ctgtacgacg gcaccgcctc ggacggcgag
      541 caggcgctct acatgagcat gccctgcccc taa
//
