LOCUS       sequence-447985-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for yeast gene knockout, uses auxotrophic marker 
            his5 (Schizosaccharomyces Pombe) (complements S.c. his3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Feng Y-H, Song J-Z, Zhu J, Xie Z
  TITLE     Template plasmids optimized for deletion of multiple genes in yeast 
            Saccharomyces cerevisiae.
  JOURNAL   Microbiol Spectr. 2024 Oct 10:e0132024. doi: 
            10.1128/spectrum.01320-24.
  PUBMED    39387598
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr. 2024 Oct 10:e0132024. doi: 10.1128/spectrum.01320-24."
COMMENT     Sequence Label: SJZ6-HIS5
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..654
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
ORIGIN
        1 atgggtagga gggcttttgt agaaagaaat acgaacgaaa cgaaaatcag cgttgccatc
       61 gctttggaca aagctccctt acctgaagag tcgaatttta ttgatgaact tataacttcc
      121 aagcatgcaa accaaaaggg agaacaagta atccaagtag acacgggaat tggattcctg
      181 gatcacatgt atcatgcact ggctaaacat gcaggctgga gcttacgact ttactcaaga
      241 ggtgatttaa tcatcgatga tcatcacact gcagaagata ctgctattgc acttggtatt
      301 gcattcaagc aggctatggg taactttgcc ggcgttaaaa gatttggaca tgcttattgt
      361 ccacttgacg aagccctttc tagaagcgta gttgacttgt cgggacggcc ctatgctgtt
      421 atcgatttgg gattaaagcg tgaaaaggtt ggggaattgt cctgtgaaat gatccctcac
      481 ttactatatt ccttttcggt agcagctgga attactttgc atgttacctg cttatatggt
      541 agtaatgacc atcatcgtgc tgaaagcgct tttaaatctc tggctgttgc catgcgcgcg
      601 gctactagtc ttactggaag ttctgaagtc ccaagcacga agggagtgtt gtaa
//
