LOCUS       sequence-447991-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for yeast gene knockout, uses auxotrophic marker 
            LEU2 (Kluyveromyces lactis).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Feng Y-H, Song J-Z, Zhu J, Xie Z
  TITLE     Template plasmids optimized for deletion of multiple genes in yeast 
            Saccharomyces cerevisiae.
  JOURNAL   Microbiol Spectr. 2024 Oct 10:e0132024. doi: 
            10.1128/spectrum.01320-24.
  PUBMED    39387598
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr. 2024 Oct 10:e0132024. doi: 10.1128/spectrum.01320-24."
COMMENT     Sequence Label: SJZ10-LEU2
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtctaaga atatcgttgt cctaccgggt gatcacgtcg gtaaagaagt tactgacgaa
       61 gctattaagg tcttgaatgc cattgctgaa gtccgtccag aaattaagtt caatttccaa
      121 catcacttga tcgggggtgc tgccatcgat gccactggca ctcctttacc agatgaagct
      181 ctagaagcct ctaagaaagc cgatgctgtc ttactaggtg ctgttggtgg tccaaaatgg
      241 ggtacgggcg cagttagacc agaacaaggt ctattgaaga tcagaaagga attgggtcta
      301 tacgccaact tgagaccatg taactttgct tctgattctt tactagatct ttctcctttg
      361 aagcctgaat atgcaaaggg taccgatttc gtcgtcgtta gagaattggt tggtggtatc
      421 tactttggtg aaagaaaaga agatgaaggt gacggagttg cttgggactc tgagaaatac
      481 agtgttcctg aagttcaaag aattacaaga atggctgctt tcttggcatt gcaacaaaac
      541 ccaccattac caatctggtc tcttgacaag gctaacgtgc ttgcctcttc cagattgtgg
      601 agaaagactg ttgaagaaac catcaagact gagttcccac aattaactgt tcagcaccaa
      661 ttgatcgact ctgctgctat gattttggtt aaatcaccaa ctaagctaaa cggtgttgtt
      721 attaccaaca acatgtttgg tgatattatc tccgatgaag cctctgttat tccaggttct
      781 ttgggtttat taccttctgc atctctagct tccctacctg acactaacaa ggcattcggt
      841 ttgtacgaac catgtcatgg ttctgcccca gatttaccag caaacaaggt taacccaatt
      901 gctaccatct tatctgcagc tatgatgttg aagttatcct tggatttggt tgaagaaggt
      961 agggctcttg aagaagctgt tagaaatgtc ttggatgcag gtgtcagaac cggtgacctt
     1021 ggtggttcta actctaccac tgaggttggc gatgctatcg ccaaggctgt caaggaaatc
     1081 ttggcttaa
//
