LOCUS       sequence-44801-e        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Dieriks B, Van Oostveldt P
  TITLE     Spatiotemporal behavior of nuclear cyclophilin B indicates a role in
            RNA transcription.
  JOURNAL   Int J Mol Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937.
  PUBMED    22426501
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937."
COMMENT     Sequence Label: PcDNA-DEST53-CypB-Del2 N-term GFP
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(549..648)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(665..684)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(666..684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(689..705)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(689..705)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(807..826)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(885..904)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ccttaaggtt aagggggcaa gtcaccgtca ggtgtatttt gacctacgaa ttggagatga
       61 agatgtaggc cgggtgatct ttggtctctt cggaaagact gttccaaaaa cagtggataa
      121 ttttgtggcc ttagctacag gagagaaagg atttggctac aaaaacagca aattccatcg
      181 tgtaatcaag gacttcatga tccagggcgg agacttcacc aggggagatg gcacaggagg
      241 aaagagcatc tacggtgagc gcttccccga tgagaacttc aaactgaagc actacgggcc
      301 tggctgggtg agcatggcca acgcaggcaa agacaccaac ggctcccagt tcttcatcac
      361 gacagtcaag acagcctggc tagatggcaa gcatgtggtg tttggcaaag ttctagaggg
      421 catggaggtg gtgcggaagg tggagagcac caagacagac agccgggata aacccctgaa
      481 ggatgtgatc atcgcagact gcggcaagat cgaggtggag aagccctttg ccatcgccaa
      541 ggagtagaac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
      601 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
      661 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
      721 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
      781 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
      841 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
      901 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
      961 agagttgttt tctgaaacat ggcaaaggta gcgttgctca atgatgttac agatgagatg
     1021 gttcagacta aactggctga cggaatttat gcctctccga ccatcagcat ttaatccgta
     1081 ctctgatgat gcatgcttac tcaccactgc cgatccccgg aaaaaaacta gcat
//
