LOCUS       sequence-448012-       11370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To make gene fusions with Arabidopsis codon-optimized mScarlet3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11370)
  TITLE     Siddiqi August 2024 Plasmids
REFERENCE   2  (bases 1 to 11370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGENT_GW_aco_mScarlet3
FEATURES             Location/Qualifiers
     source          1..11370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    115..139
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    314..335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(350..355,356..373,374..380)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    388..404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     393..415
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     412..428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     412..428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    463..587
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(612..617,618..635,636..642)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             696..1355
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(762..781)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1697..2002
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1881..1900
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2043..2167)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2184..2870
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(3127..3144)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3127..3143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3136..3158)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3372..4048
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     3985..4005
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4111..4644
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     polyA_signal    4701..4875
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4953..4977
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5498..6289
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6535..7123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7024..7043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7277..7294
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7309..7449
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7375..7394)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7535..7557
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7793..7987
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8053..9126)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9555..10184)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
      121 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
      181 atccaagctc aagctgctct agagcgctct agccaatacg caaaccgcct ctccccgcgc
      241 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      301 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      361 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      421 gctatgacat gattacgaat tctcgagggg ggggcccgcg ccacaagttt gtacaaaaaa
      481 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
      541 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
      601 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
      661 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
      721 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
      781 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
      841 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
      901 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
      961 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     1021 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     1081 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     1141 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     1201 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     1261 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     1321 aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag
     1381 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     1441 tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt
     1501 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     1561 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     1621 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     1681 aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     1741 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     1801 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     1861 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     1921 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     1981 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     2041 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     2101 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     2161 aagtggtgct cgaggccggt accatggata gtactgaggc agttatcaag gaatttatga
     2221 gatttaaagt tcacatggag ggctcaatga atggacacga gttcgagatt gaaggtgagg
     2281 gcgagggcag accttacgag ggaacccaaa ctgcaaagct gagggttact aagggaggtc
     2341 cattgccatt ctcgtgggac atcttgtccc ctcagttcat gtatggatcg agagccttta
     2401 cgaaacatcc ggcagatatc cctgattatt ggaaacaatc tttccctgag ggatttaagt
     2461 gggagagagt gatgaacttt gaggacggtg gtgcagtatc ggttgctcaa gatacatcac
     2521 tggaagatgg aacgcttata tacaaagtga agttgagagg aacaaacttc ccaccagacg
     2581 gaccggtaat gcaaaagaag acgatggggt gggaagcaag cacagagaga ctatatccgg
     2641 aagacgtcgt cttgaagggt gatattaaga tggccctcag gttgaaagac ggtggaaggt
     2701 atttagcaga cttcaaaacg acctacagag ccaagaagcc tgttcagatg ccaggtgcat
     2761 ttaatatcga tcgtaagctg gatatcacat cccacaatga ggactacacg gttgtcgagc
     2821 aatatgaaag atcagtggct aggcattcga caggtggatc aggtgggtct tagggtaccc
     2881 ggggatcctc tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta
     2941 tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg
     3001 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
     3061 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacctgca ggcatgcaag
     3121 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     3181 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     3241 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgccaac
     3301 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
     3361 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
     3421 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
     3481 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
     3541 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
     3601 tcaaagcaag tggattgatg tgaacatggt ggagcacgac actctcgtct actccaagaa
     3661 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
     3721 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
     3781 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
     3841 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     3901 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
     3961 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     4021 ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa atctatctct
     4081 ctcgagcttt cgcagatctg tcgatcgacc atggtacgca gcagcaacga tgttacgcag
     4141 cagggcagtc gccctaaaac aaagttaggt ggctcaagta tgggcatcat tcgcacatgt
     4201 aggctcggcc ctgaccaagt caaatccatg cgggctgctc ttgatctttt cggtcgtgag
     4261 ttcggagacg tagccaccta ctcccaacat cagccggact ccgattacct cgggaacttg
     4321 ctccgtagta agacattcat cgcgcttgct gccttcgacc aagaagcggt tgttggcgct
     4381 ctcgcggctt acgttctgcc caagtttgag cagccgcgta gtgagatcta tatctatgat
     4441 ctcgcagtct ccggcgagca ccggaggcag ggcattgcca ccgcgctcat caatctcctc
     4501 aagcatgagg ccaacgcgct tggtgcttat gtgatctacg tgcaagcaga ttacggtgac
     4561 gatcccgcag tggctctcta tacaaagttg ggcatacggg aagaagtgat gcactttgat
     4621 atcgacccaa gtaccgccac ctaacaattc gttcaagccg agatcggctt cccggcctag
     4681 agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga
     4741 attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     4801 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     4861 actaaaatcc agatcccccg aattaattcg gcgttaattc agtacattaa aaacgtccgc
     4921 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     4981 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     5041 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     5101 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     5161 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg
     5221 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     5281 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     5341 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     5401 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     5461 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
     5521 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     5581 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     5641 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     5701 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     5761 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     5821 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     5881 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     5941 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     6001 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     6061 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     6121 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     6181 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     6241 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     6301 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     6361 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     6421 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     6481 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6541 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6601 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6661 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     6721 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6781 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6841 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6901 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6961 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7021 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7081 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7141 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7201 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7261 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     7321 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     7381 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     7441 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7501 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7561 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     7621 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     7681 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     7741 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     7801 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     7861 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     7921 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     7981 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     8041 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     8101 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     8161 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     8221 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     8281 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     8341 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     8401 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     8461 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     8521 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     8581 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     8641 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     8701 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     8761 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     8821 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     8881 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     8941 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     9001 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     9061 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     9121 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
     9181 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     9241 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
     9301 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
     9361 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
     9421 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
     9481 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
     9541 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
     9601 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
     9661 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
     9721 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
     9781 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
     9841 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
     9901 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
     9961 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
    10021 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
    10081 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
    10141 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
    10201 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
    10261 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
    10321 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
    10381 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
    10441 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
    10501 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
    10561 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
    10621 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
    10681 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
    10741 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
    10801 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
    10861 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
    10921 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
    10981 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
    11041 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
    11101 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
    11161 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
    11221 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
    11281 cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
    11341 atagcagcgg aggggttgga tcaaagtact
//
