LOCUS       sequence-448019-       20008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To clone entry vector with CRISPR guide RNA via Gateway cloning; it 
            has an Egg cell-specific expression of Cas9 and the plant is 
            selected by the red color due to constitutive expression of RUBY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20008)
  TITLE     Siddiqi August 2024 Plasmids
REFERENCE   2  (bases 1 to 20008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGW_RUBY_EggCas9
FEATURES             Location/Qualifiers
     source          1..20008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    319..343
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1779..1844
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1851..1871
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1896..5996
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1896..5996
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             5909..5917
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5997..6044
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    6712..6836
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6861..6866,6867..6884,6885..6891)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6945..7625
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(7011..7030)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7945..8250
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8129..8148
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8291..8415)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(9115..13065)
                     /label=RUBY
     CDS             complement(10618..10674)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(11509..11565)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        complement(13074..13750)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(13117..13137)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    13765..13789
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             15120..15749
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             16178..17251
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      17317..17511
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(17747..17769)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    17855..17995
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     17910..17929
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(18010..18027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18181..18769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18261..18280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(19015..19806)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc
       61 cactgggttc gtgcccgaat tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
      121 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
      181 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
      241 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
      301 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
      361 acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat tgaattcgaa
      421 taaaagcatt tgcgtttggt ttatcattgc gtttatacaa ggacagagat ccactgagct
      481 ggaatagctt aaaaccatta tcagaacaaa ataaaccatt ttttgttaag aatcagagca
      541 tagtaaacaa cagaaacaac ctaagagagg taacttgtcc aagaagatag ctaattatat
      601 ctattttata aaagttatca tagtttgtaa gtcacaaaag atgcaaataa cagagaaact
      661 aggagacttg agaatataca ttcttgtata tttgtattcg agattgtgaa aatttgacca
      721 taagtttaaa ttcttaaaaa gatatatctg atctaggtga tggttataga ctgtaatttt
      781 accacatgtt taatgatgga tagtgacaca catgacacat cgacaacact atagcatctt
      841 atttagatta caacatgaaa tttttctgta atacatgtct ttgtacataa tttaaaagta
      901 attcctaaga aatatattta tacaaggagt ttaaagaaaa catagcataa agttcaatga
      961 gtagtaaaaa ccatatacag tatatagcat aaagttcaat gagtttatta caaaagcatt
     1021 ggttcacttt ctgtaacacg acgttaaacc ttcgtctcca ataggagcgc tactgattca
     1081 acatgccaat atatactaaa tacgtttcta cagtcaaatg ctttaacgtt tcatgattaa
     1141 gtgactattt accgtcaatc ctttcccatt cctcccacta atccaacttt ttaattactc
     1201 ttaaatcacc actaagctag taacgcctat catgaattag ctctactaaa tctagcaacc
     1261 tttcaaattt gcagtattgc aggtgtctct gtgtctttaa aatagttgcc ttatgatttc
     1321 ttcggtttca agatgatcaa atagttatag atttcatgct cacacatgct cattagatgt
     1381 gtacatactt tacttaccca aatctatttt ctcgcaaaga ttttgatggt aaagctgatt
     1441 tggttctatt gaactaaatc aaacgagttt cagactgagt gattctaatc cggcccatta
     1501 gcccctaaac agacccacta attacgcagc ttttaataga gtaattacac ctagtttacc
     1561 cactaaacca ctaagcacta attatctcac aatctaatga gcttccctcg taattacttg
     1621 ggctttcact ctaccattta tttgtaacag tcaagtctct actgtctcta tataaactct
     1681 ctaaagttaa cacacaattc tcatcacaaa caaatcaacc aaagcaactt ctactctttc
     1741 ttctttcgac cttatcaatc tgttgagaaa tctagatgga ttacaaggac cacgacgggg
     1801 attacaagga ccacgacatt gattacaagg atgatgatga caagatggct ccgaagaaga
     1861 agaggaaggt tggcatccac ggggtgccag ctgctgacaa gaagtactcg atcggcctcg
     1921 atattgggac taactctgtt ggctgggccg tgatcaccga cgagtacaag gtgccctcaa
     1981 agaagttcaa ggtcctgggc aacaccgatc ggcattccat caagaagaat ctcattggcg
     2041 ctctcctgtt cgacagcggc gagacggctg aggctacgcg gctcaagcgc accgcccgca
     2101 ggcggtacac gcgcaggaag aatcgcatct gctacctgca ggagattttc tccaacgaga
     2161 tggcgaaggt tgacgattct ttcttccaca ggctggagga gtcattcctc gtggaggagg
     2221 ataagaagca cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt gcctaccacg
     2281 agaagtaccc tacgatctac catctgcgga agaagctcgt ggactccaca gataaggcgg
     2341 acctccgcct gatctacctc gctctggccc acatgattaa gttcaggggc catttcctga
     2401 tcgaggggga tctcaacccg gacaatagcg atgttgacaa gctgttcatc cagctcgtgc
     2461 agacgtacaa ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc gacgcgaagg
     2521 ctatcctgtc cgctaggctc tcgaagtctc ggcgcctcga gaacctgatc gcccagctgc
     2581 cgggcgagaa gaagaacggc ctgttcggga atctcattgc gctcagcctg gggctcacgc
     2641 ccaacttcaa gtcgaatttc gatctcgctg aggacgccaa gctgcagctc tccaaggaca
     2701 catacgacga tgacctggat aacctcctgg cccagatcgg cgatcagtac gcggacctgt
     2761 tcctcgctgc caagaatctg tcggacgcca tcctcctgtc tgatattctc agggtgaaca
     2821 ccgagattac gaaggctccg ctctcagcct ccatgatcaa gcgctacgac gagcaccatc
     2881 aggatctgac cctcctgaag gcgctggtca ggcagcagct ccccgagaag tacaaggaga
     2941 tcttcttcga tcagtcgaag aacggctacg ctgggtacat tgacggcggg gcctctcagg
     3001 aggagttcta caagttcatc aagccgattc tggagaagat ggacggcacg gaggagctgc
     3061 tggtgaagct caatcgcgag gacctcctga ggaagcagcg gacattcgat aacggcagca
     3121 tcccacacca gattcatctc ggggagctgc acgctatcct gaggaggcag gaggacttct
     3181 accctttcct caaggataac cgcgagaaga tcgagaagat tctgactttc aggatcccgt
     3241 actacgtcgg cccactcgct aggggcaact cccgcttcgc ttggatgacc cgcaagtcag
     3301 aggagacgat cacgccgtgg aacttcgagg aggtggtcga caagggcgct agcgctcagt
     3361 cgttcatcga gaggatgacg aatttcgaca agaacctgcc aaatgagaag gtgctcccta
     3421 agcactcgct cctgtacgag tacttcacag tctacaacga gctgactaag gtgaagtatg
     3481 tgaccgaggg catgaggaag ccggctttcc tgtctgggga gcagaagaag gccatcgtgg
     3541 acctcctgtt caagaccaac cggaaggtca cggttaagca gctcaaggag gactacttca
     3601 agaagattga gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc ttcaacgcct
     3661 ccctggggac ctaccacgat ctcctgaaga tcattaagga taaggacttc ctggacaacg
     3721 aggagaatga ggatatcctc gaggacattg tgctgacact cactctgttc gaggaccggg
     3781 agatgatcga ggagcgcctg aagacttacg cccatctctt cgatgacaag gtcatgaagc
     3841 agctcaagag gaggaggtac accggctggg ggaggctgag caggaagctc atcaacggca
     3901 ttcgggacaa gcagtccggg aagacgatcc tcgacttcct gaagagcgat ggcttcgcga
     3961 accgcaattt catgcagctg attcacgatg acagcctcac attcaaggag gatatccaga
     4021 aggctcaggt gagcggccag ggggactcgc tgcacgagca tatcgcgaac ctcgctggct
     4081 cgccagctat caagaagggg attctgcaga ccgtgaaggt tgtggacgag ctggtgaagg
     4141 tcatgggcag gcacaagcct gagaacatcg tcattgagat ggcccgggag aatcagacca
     4201 cgcagaaggg ccagaagaac tcacgcgaga ggatgaagag gatcgaggag ggcattaagg
     4261 agctggggtc ccagatcctc aaggagcacc cggtggagaa cacgcagctg cagaatgaga
     4321 agctctacct gtactacctc cagaatggcc gcgatatgta tgtggaccag gagctggata
     4381 ttaacaggct cagcgattac gacgtcgatc atatcgttcc acagtcattc ctgaaggatg
     4441 actccattga caacaaggtc ctcaccaggt cggacaagaa ccggggcaag tctgataatg
     4501 ttccttcaga ggaggtcgtt aagaagatga agaactactg gcgccagctc ctgaatgcca
     4561 agctgatcac gcagcggaag ttcgataacc tcacaaaggc tgagaggggc gggctctctg
     4621 agctggacaa ggcgggcttc atcaagaggc agctggtcga gacacggcag atcactaagc
     4681 acgttgcgca gattctcgac tcacggatga acactaagta cgatgagaat gacaagctga
     4741 tccgcgaggt gaaggtcatc accctgaagt caaagctcgt ctccgacttc aggaaggatt
     4801 tccagttcta caaggttcgg gagatcaaca attaccacca tgcccatgac gcgtacctga
     4861 acgcggtggt cggcacagct ctgatcaaga agtacccaaa gctcgagagc gagttcgtgt
     4921 acggggacta caaggtttac gatgtgagga agatgatcgc caagtcggag caggagattg
     4981 gcaaggctac cgccaagtac ttcttctact ctaacattat gaatttcttc aagacagaga
     5041 tcactctggc caatggcgag atccggaagc gccccctcat cgagacgaac ggcgagacgg
     5101 gggagatcgt gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt ctctccatgc
     5161 cacaagtgaa tatcgtcaag aagacagagg tccagactgg cgggttctct aaggagtcaa
     5221 ttctgcctaa gcggaacagc gacaagctca tcgcccgcaa gaaggactgg gatccgaaga
     5281 agtacggcgg gttcgacagc cccactgtgg cctactcggt cctggttgtg gcgaaggttg
     5341 agaagggcaa gtccaagaag ctcaagagcg tgaaggagct gctggggatc acgattatgg
     5401 agcgctccag cttcgagaag aacccgatcg atttcctgga ggcgaagggc tacaaggagg
     5461 tgaagaagga cctgatcatt aagctcccca agtactcact cttcgagctg gagaacggca
     5521 ggaagcggat gctggcttcc gctggcgagc tgcagaaggg gaacgagctg gctctgccgt
     5581 ccaagtatgt gaacttcctc tacctggcct cccactacga gaagctcaag ggcagccccg
     5641 aggacaacga gcagaagcag ctgttcgtcg agcagcacaa gcattacctc gacgagatca
     5701 ttgagcagat ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat ctggataagg
     5761 tcctctccgc gtacaacaag caccgcgaca agccaatcag ggagcaggct gagaatatca
     5821 ttcatctctt caccctgacg aacctcggcg cccctgctgc tttcaagtac ttcgacacaa
     5881 ctatcgatcg caagaggtac acaagcacta aggaggtcct ggacgcgacc ctcatccacc
     5941 agtcgattac cggcctctac gagacgcgca tcgacctgtc tcagctcggg ggcgacaagc
     6001 ggccagcggc gacgaagaag gcggggcagg cgaagaagaa gaagtgagct cagagctttc
     6061 gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt cattgcgcac
     6121 acaccagaat cctactgagt ttgagtatta tggcattggg aaaactgttt ttcttgtacc
     6181 atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa
     6241 tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa
     6301 tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca
     6361 aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat
     6421 gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac aaatatattt
     6481 tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg tgttttagac
     6541 atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta atcattgctt
     6601 tataattata gttatactca tggatttgta gttgagtatg aaaatatttt ttaatgcatt
     6661 ttatgacttg ccaattgatt gacaacgagc tccctaggcc tgttatccct aacaagtttg
     6721 tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
     6781 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg
     6841 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
     6901 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     6961 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     7021 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     7081 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     7141 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     7201 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     7261 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     7321 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     7381 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     7441 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     7501 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     7561 atgaattaca acagtactgc gatgagtggc aggcggggcg taatctagag gatccggctt
     7621 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     7681 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     7741 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     7801 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     7861 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     7921 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     7981 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     8041 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     8101 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     8161 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     8221 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     8281 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     8341 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     8401 cttgtacaaa gtggttcgat aattccgatc cagcctaggc ccgggcctga ggacgcgtcc
     8461 atggttaatt aagacgtccg gaccgactag tggatcctct agagtcgacc tgcaggcatg
     8521 caagcttgtt tggggatcta gtgttttact cctcatatta acttcggtca ttagaggcca
     8581 cgatttgaca catttttact caaaacaaaa tgtttgcata tctcttataa tttcaaattc
     8641 aacacacaac aaataagaga aaaaacaaat aatattaatt tgagaatgaa caaaaggacc
     8701 atatcattca ttaactcttc tccatccatt tccatttcac agttcgatag cgaaaaccga
     8761 ataaaaaaca cagtaaatta caagcacaac aaatggtaca agaaaaacag ttttcccaat
     8821 gccataatac tcgaacaagc tagcttactc agttaggtct tatctttaat catattccat
     8881 agtccatacc atagcacata cagtagttat atgctggaga agagatccaa caaaacattc
     8941 acaatggatt atagaaacat ttgtttattc attataatga gatgttacat tcatttaata
     9001 ttagaaaaag ccacaaattc ataacacaac aagccaagaa aaaaacacaa acttaagcac
     9061 acaacctttt tatttgacac accaaatatt tcatcttcat cttcatattc actatcactg
     9121 gaggcttggc tcaagtttgg cctgcatgac ggcgtcgagg aaatcgtcga tcgccttcac
     9181 ggaggagccc ttcagctctt tttcctcgtt cacggcgtcc ttgagctttt tgcccatttc
     9241 cacggcgcga ttcttcatct cccagccttt ggagccctcg ttgcgctcaa ggatcatctc
     9301 gacgatgcgc ttgaccttct ctttggagat ctcgccttcg aggcctctag caagctcaac
     9361 cgcgacgccc atctcatctt cgaggtactt gaggttgtag gcctgctcgg cagcaagtgg
     9421 ccatccaagc atcggaacgc cctcgcgaag agactcaagg atgctgttcc atccgcagtg
     9481 ggtgagaaag ccgccgatgg attcatggtt gaggatctca agctgcgggc cgagcttgta
     9541 cacgagcttg ccctgtttct tcaccttcat gcgttcctca aagccctctg gcagccactc
     9601 tgggcgcatc tcttcgttga tgtcgaagcc gaacggggct ctgatcaccc agaggaatgg
     9661 cttctcggag gactcaaggc cagcagcgag ttccatcatc tgcgtcgggc tgatcgtgtt
     9721 ctgggagccg aaggagatgt agaggacgga gtccggctct ttgaggctga gccactgcac
     9781 gaactcggcg cctgttgaat tgttgtcgga ggaggagtgc tgcactgggg aagcaataag
     9841 tgggccgatg ccccagatcg gcagcttggt gtagttgcgg aggatggaga agccgagtgt
     9901 ctcgatctct tccacggagt tgcagagcca gccaaaagac ttcatggact ggcggagctg
     9961 cggctggaaa tacttggacc aatcgtcgga gccgtcggca tatctgagga accgatggag
    10021 ctgggacctg cggaacttat ggttctctgg gaagcctggc agcgggaact cttggtcgtc
    10081 ggagtagttc tggtgcggga gatcgttcca gatggacacg taggcggatg tgccataagc
    10141 gccgcctgtt gtgaacacca cgcctgtaga gcccacatcc ttggccacct ggtcaaccca
    10201 gccgaggaac acatcatgga tcacgcagag tggtggcctg gctgggctga aatgcctgga
    10261 gatgtaatcg cggaggtgcg gatcgaggct gatggtggaa tggaagaggg acaccacgag
    10321 cgggagtgtg agcttatcgg tgttctcgat gcccggtggg agtccgtggt tggtggagtt
    10381 gaatggcagc tcgacgatcc tgatgccgct ggaggagtag gaatggtggt gaaggaggtg
    10441 cctgaggaag ccggcattga gcggagttgt gagcagggtg atgatcacgt gggagcgctt
    10501 gtagaggaac atggcgagtt cgagaaatgg cctgaggtgg ccctgcgcca tgaacgggat
    10561 catgaggatg tgctgtgtct cgccctcgcc gttggtgttc atcttgatgg cggtcatggg
    10621 gccaggattt tcctcaacat ctcctgcttg cttgagcagg ctaaagttgg ttgctccgga
    10681 tccggcggag gtgaacttgt aggagccgtg gcaaagtgtg ccatgatccc aggaggagtg
    10741 gatcagctcg gccttccact tttcaccagc agcgccgagc acaacgtgga gcgggtagaa
    10801 gtgctctgga aatggatggg cgagcttcca gttcggggcc ttgctctcgt agatgttcac
    10861 ttcctcgaag cggccgttga tgagggcctt cctaagccaa gaatcgaagg cagcggccca
    10921 aggggcaaca ccatcgaagt agtgcggtgt ctcatcgagt ggatgtgtgg cgctgccgga
    10981 gccaataatg agcacgccat cattcttgag cggcgcgagg gctctgccga gattgtagtg
    11041 gtaggtgccg tcgagatgtg gctgaactga gagctggcac accgggatgt ccgcctctgg
    11101 atacatgagc atgagtggaa cccaggcgcc gtgatcaagg cccctctttt ggtctgtctc
    11161 ggctgtctcg aacccggact tcttgaggat ctcttccacc tttctcgcaa gctctggctc
    11221 gcctggagct gggtacttga actggtacat ggcggctggg tagtcgtcga agtcgtggat
    11281 ggtgtcgttg atgtgcacgg cgttcacggt tggcttcact gtctcccagt ggccggagat
    11341 gatgaggatg gccttcggct tcttggagaa aatcttctcg cgccatgtct cgaagaacgg
    11401 cctgagcgga tgtgtatcct cgacggtgag gatcgggttg ccgtgggtga tgaagaagga
    11461 ctctttgatc atctggtcgt tggcgtcctc gccgttcatc atcttcatag gcccggggtt
    11521 ctcttctaca tcacctgcct gcttaaggag gctaaaattg gtagctccgc taccgtagcg
    11581 cggaatcggg atgagcttga gcggcttggt cttttggagc gcaatgccga acttctcgtc
    11641 catgtcgagg tccttcgggg agatgtcgcc ttcgagcttc cagttgaaga actggaggag
    11701 tgtggcgagc atgagggtga gcatcctgat ggcgagattc atgcctgggc aaattctgcg
    11761 gccagcgcca aatggaagga ggccgaaatc gcggccctta acatcgatct cgcagccgat
    11821 gaagcgctct gggctgaaaa tatcggcgtt ctgccaggcg tttgggtccc tgccaatggc
    11881 ccagaggttc acgaggatct gggcatcctt cggcacgatg tagccgtaca gctcgacatc
    11941 ggtgtcggcc tttcttggga gcaagaacac ggtcggcgga tggaggcgga gtgtctcttt
    12001 gatgatcgcc tggaggtacg gcaggttgat gatgtcggac tcttggatct gcttgtcctt
    12061 gccgaggacc tgcttgattt cctcttgggc cttttccatc atctctggat tgcggatcag
    12121 ctcggtcatc acccactcga atgtggagga tgtggtatcg gtgccggcgt cgaagatgtc
    12181 cacgaggagg tggttgatct cgcccatcgt cagctcgttc tgcttgaaca gctggaggag
    12241 cacatcgagc acgtcgtcgg tggtggtggt agttgtggtg gaggaatctg gggcgagtct
    12301 ctcgcagatg atgccctgga acacggcgat cagcttgtcg aaggagcagg cgagccttct
    12361 tctaatgcca gatgggtcaa cgcagccgag aatcgggaag tagtcggcgt agttcggctt
    12421 gcctatatct tccatgatgt tccagatcag ctctttgaac tcttggctgg tatgggactt
    12481 gtggtgcgcc agctcgacgc tgaagaacag cttgctgagg aggttgaggc tggtcgtaaa
    12541 ggcggccttg ccaatatcca cggcctggcc tttctgggcg cactcttgaa cgtactcgta
    12601 gagctgctgc accttggcgt gcctgaatgt ctggcaggca tcgagtctct gtggggagag
    12661 cagatgcacg gcggtaatct tgcggaagtt gcgccacttc ggagacaccg ggagccaaga
    12721 catggtgagc ttgtggtggt cgccggctgt aacactattc gggatcgtgc ggttggagag
    12781 cgggtgatcc ttcttgagga acatctcttt ggccacgtcg gcagaggaca caacgatggt
    12841 ggtcacagag ccgagtctga gggaaatgag tgggccgtga atcttggcga ggttggcgaa
    12901 ggaccgatgc ggcttcttgc ccacctcaag gatgttgccg atgatcggaa gcggctttgg
    12961 tcctggcgga agcagcttgg tggtctgctg ggagaacagc agcttgatga agtggaagct
    13021 gatgaaccag atcgcgagga tcatggcgag ggtcgcatga tccatactag ttttcagcgt
    13081 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
    13141 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
    13201 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
    13261 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
    13321 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
    13381 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
    13441 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttc
    13501 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
    13561 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
    13621 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
    13681 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
    13741 aaaagtctca aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
    13801 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
    13861 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt taaagtactt
    13921 taaagtactt taaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
    13981 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
    14041 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
    14101 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
    14161 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
    14221 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
    14281 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
    14341 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
    14401 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
    14461 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
    14521 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
    14581 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
    14641 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
    14701 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
    14761 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
    14821 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
    14881 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
    14941 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
    15001 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
    15061 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
    15121 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
    15181 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
    15241 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
    15301 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
    15361 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
    15421 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
    15481 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
    15541 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
    15601 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
    15661 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
    15721 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
    15781 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
    15841 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
    15901 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
    15961 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
    16021 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
    16081 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct
    16141 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
    16201 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
    16261 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
    16321 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
    16381 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
    16441 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
    16501 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
    16561 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
    16621 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
    16681 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
    16741 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
    16801 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
    16861 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
    16921 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
    16981 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
    17041 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
    17101 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
    17161 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
    17221 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
    17281 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
    17341 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
    17401 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
    17461 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
    17521 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
    17581 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
    17641 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
    17701 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    17761 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    17821 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
    17881 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    17941 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
    18001 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    18061 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    18121 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    18181 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    18241 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    18301 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    18361 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    18421 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    18481 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    18541 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    18601 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    18661 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    18721 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    18781 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    18841 catgcatgat atatctccca atttgtgtag ggcttattat gcacgcttaa aaataataaa
    18901 agcagacttg acctgatagt ttggctgtga gcaattatgt gcttagtgca tctaacgctt
    18961 gagttaagcc gcgccgcgaa gcggcgtcgg cttgaacgaa tttctagcta gacattattt
    19021 gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc
    19081 gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac
    19141 gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc
    19201 gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc
    19261 atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa
    19321 tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac
    19381 gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc
    19441 gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc
    19501 tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag
    19561 aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg
    19621 cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt
    19681 caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg
    19741 gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc
    19801 ccccatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct
    19861 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
    19921 agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc
    19981 accgctgcgt tcggtcaagg ttctggac
//
