LOCUS       sequence-448020-       19235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. 
            The transgenics can be selected by red color appearance of plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19235)
  TITLE     Siddiqi August 2024 Plasmids
REFERENCE   2  (bases 1 to 19235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAG1guide_RUBY_EggCas9
FEATURES             Location/Qualifiers
     source          1..19235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    319..343
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1779..1844
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1851..1871
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1896..5996
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1896..5996
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             5909..5917
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5997..6044
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    6712..6736
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        7155..7230
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(7617..7641)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(8341..12291)
                     /label=RUBY
     CDS             complement(9844..9900)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(10735..10791)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        complement(12300..12977)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(12343..12363)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    12992..13016
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14347..14976
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             15405..16478
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      16544..16738
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(16974..16996)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    17082..17222
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     17137..17156
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(17237..17254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17408..17996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17488..17507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(18242..19033)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc
       61 cactgggttc gtgcccgaat tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
      121 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
      181 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
      241 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
      301 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
      361 acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat tgaattcgaa
      421 taaaagcatt tgcgtttggt ttatcattgc gtttatacaa ggacagagat ccactgagct
      481 ggaatagctt aaaaccatta tcagaacaaa ataaaccatt ttttgttaag aatcagagca
      541 tagtaaacaa cagaaacaac ctaagagagg taacttgtcc aagaagatag ctaattatat
      601 ctattttata aaagttatca tagtttgtaa gtcacaaaag atgcaaataa cagagaaact
      661 aggagacttg agaatataca ttcttgtata tttgtattcg agattgtgaa aatttgacca
      721 taagtttaaa ttcttaaaaa gatatatctg atctaggtga tggttataga ctgtaatttt
      781 accacatgtt taatgatgga tagtgacaca catgacacat cgacaacact atagcatctt
      841 atttagatta caacatgaaa tttttctgta atacatgtct ttgtacataa tttaaaagta
      901 attcctaaga aatatattta tacaaggagt ttaaagaaaa catagcataa agttcaatga
      961 gtagtaaaaa ccatatacag tatatagcat aaagttcaat gagtttatta caaaagcatt
     1021 ggttcacttt ctgtaacacg acgttaaacc ttcgtctcca ataggagcgc tactgattca
     1081 acatgccaat atatactaaa tacgtttcta cagtcaaatg ctttaacgtt tcatgattaa
     1141 gtgactattt accgtcaatc ctttcccatt cctcccacta atccaacttt ttaattactc
     1201 ttaaatcacc actaagctag taacgcctat catgaattag ctctactaaa tctagcaacc
     1261 tttcaaattt gcagtattgc aggtgtctct gtgtctttaa aatagttgcc ttatgatttc
     1321 ttcggtttca agatgatcaa atagttatag atttcatgct cacacatgct cattagatgt
     1381 gtacatactt tacttaccca aatctatttt ctcgcaaaga ttttgatggt aaagctgatt
     1441 tggttctatt gaactaaatc aaacgagttt cagactgagt gattctaatc cggcccatta
     1501 gcccctaaac agacccacta attacgcagc ttttaataga gtaattacac ctagtttacc
     1561 cactaaacca ctaagcacta attatctcac aatctaatga gcttccctcg taattacttg
     1621 ggctttcact ctaccattta tttgtaacag tcaagtctct actgtctcta tataaactct
     1681 ctaaagttaa cacacaattc tcatcacaaa caaatcaacc aaagcaactt ctactctttc
     1741 ttctttcgac cttatcaatc tgttgagaaa tctagatgga ttacaaggac cacgacgggg
     1801 attacaagga ccacgacatt gattacaagg atgatgatga caagatggct ccgaagaaga
     1861 agaggaaggt tggcatccac ggggtgccag ctgctgacaa gaagtactcg atcggcctcg
     1921 atattgggac taactctgtt ggctgggccg tgatcaccga cgagtacaag gtgccctcaa
     1981 agaagttcaa ggtcctgggc aacaccgatc ggcattccat caagaagaat ctcattggcg
     2041 ctctcctgtt cgacagcggc gagacggctg aggctacgcg gctcaagcgc accgcccgca
     2101 ggcggtacac gcgcaggaag aatcgcatct gctacctgca ggagattttc tccaacgaga
     2161 tggcgaaggt tgacgattct ttcttccaca ggctggagga gtcattcctc gtggaggagg
     2221 ataagaagca cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt gcctaccacg
     2281 agaagtaccc tacgatctac catctgcgga agaagctcgt ggactccaca gataaggcgg
     2341 acctccgcct gatctacctc gctctggccc acatgattaa gttcaggggc catttcctga
     2401 tcgaggggga tctcaacccg gacaatagcg atgttgacaa gctgttcatc cagctcgtgc
     2461 agacgtacaa ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc gacgcgaagg
     2521 ctatcctgtc cgctaggctc tcgaagtctc ggcgcctcga gaacctgatc gcccagctgc
     2581 cgggcgagaa gaagaacggc ctgttcggga atctcattgc gctcagcctg gggctcacgc
     2641 ccaacttcaa gtcgaatttc gatctcgctg aggacgccaa gctgcagctc tccaaggaca
     2701 catacgacga tgacctggat aacctcctgg cccagatcgg cgatcagtac gcggacctgt
     2761 tcctcgctgc caagaatctg tcggacgcca tcctcctgtc tgatattctc agggtgaaca
     2821 ccgagattac gaaggctccg ctctcagcct ccatgatcaa gcgctacgac gagcaccatc
     2881 aggatctgac cctcctgaag gcgctggtca ggcagcagct ccccgagaag tacaaggaga
     2941 tcttcttcga tcagtcgaag aacggctacg ctgggtacat tgacggcggg gcctctcagg
     3001 aggagttcta caagttcatc aagccgattc tggagaagat ggacggcacg gaggagctgc
     3061 tggtgaagct caatcgcgag gacctcctga ggaagcagcg gacattcgat aacggcagca
     3121 tcccacacca gattcatctc ggggagctgc acgctatcct gaggaggcag gaggacttct
     3181 accctttcct caaggataac cgcgagaaga tcgagaagat tctgactttc aggatcccgt
     3241 actacgtcgg cccactcgct aggggcaact cccgcttcgc ttggatgacc cgcaagtcag
     3301 aggagacgat cacgccgtgg aacttcgagg aggtggtcga caagggcgct agcgctcagt
     3361 cgttcatcga gaggatgacg aatttcgaca agaacctgcc aaatgagaag gtgctcccta
     3421 agcactcgct cctgtacgag tacttcacag tctacaacga gctgactaag gtgaagtatg
     3481 tgaccgaggg catgaggaag ccggctttcc tgtctgggga gcagaagaag gccatcgtgg
     3541 acctcctgtt caagaccaac cggaaggtca cggttaagca gctcaaggag gactacttca
     3601 agaagattga gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc ttcaacgcct
     3661 ccctggggac ctaccacgat ctcctgaaga tcattaagga taaggacttc ctggacaacg
     3721 aggagaatga ggatatcctc gaggacattg tgctgacact cactctgttc gaggaccggg
     3781 agatgatcga ggagcgcctg aagacttacg cccatctctt cgatgacaag gtcatgaagc
     3841 agctcaagag gaggaggtac accggctggg ggaggctgag caggaagctc atcaacggca
     3901 ttcgggacaa gcagtccggg aagacgatcc tcgacttcct gaagagcgat ggcttcgcga
     3961 accgcaattt catgcagctg attcacgatg acagcctcac attcaaggag gatatccaga
     4021 aggctcaggt gagcggccag ggggactcgc tgcacgagca tatcgcgaac ctcgctggct
     4081 cgccagctat caagaagggg attctgcaga ccgtgaaggt tgtggacgag ctggtgaagg
     4141 tcatgggcag gcacaagcct gagaacatcg tcattgagat ggcccgggag aatcagacca
     4201 cgcagaaggg ccagaagaac tcacgcgaga ggatgaagag gatcgaggag ggcattaagg
     4261 agctggggtc ccagatcctc aaggagcacc cggtggagaa cacgcagctg cagaatgaga
     4321 agctctacct gtactacctc cagaatggcc gcgatatgta tgtggaccag gagctggata
     4381 ttaacaggct cagcgattac gacgtcgatc atatcgttcc acagtcattc ctgaaggatg
     4441 actccattga caacaaggtc ctcaccaggt cggacaagaa ccggggcaag tctgataatg
     4501 ttccttcaga ggaggtcgtt aagaagatga agaactactg gcgccagctc ctgaatgcca
     4561 agctgatcac gcagcggaag ttcgataacc tcacaaaggc tgagaggggc gggctctctg
     4621 agctggacaa ggcgggcttc atcaagaggc agctggtcga gacacggcag atcactaagc
     4681 acgttgcgca gattctcgac tcacggatga acactaagta cgatgagaat gacaagctga
     4741 tccgcgaggt gaaggtcatc accctgaagt caaagctcgt ctccgacttc aggaaggatt
     4801 tccagttcta caaggttcgg gagatcaaca attaccacca tgcccatgac gcgtacctga
     4861 acgcggtggt cggcacagct ctgatcaaga agtacccaaa gctcgagagc gagttcgtgt
     4921 acggggacta caaggtttac gatgtgagga agatgatcgc caagtcggag caggagattg
     4981 gcaaggctac cgccaagtac ttcttctact ctaacattat gaatttcttc aagacagaga
     5041 tcactctggc caatggcgag atccggaagc gccccctcat cgagacgaac ggcgagacgg
     5101 gggagatcgt gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt ctctccatgc
     5161 cacaagtgaa tatcgtcaag aagacagagg tccagactgg cgggttctct aaggagtcaa
     5221 ttctgcctaa gcggaacagc gacaagctca tcgcccgcaa gaaggactgg gatccgaaga
     5281 agtacggcgg gttcgacagc cccactgtgg cctactcggt cctggttgtg gcgaaggttg
     5341 agaagggcaa gtccaagaag ctcaagagcg tgaaggagct gctggggatc acgattatgg
     5401 agcgctccag cttcgagaag aacccgatcg atttcctgga ggcgaagggc tacaaggagg
     5461 tgaagaagga cctgatcatt aagctcccca agtactcact cttcgagctg gagaacggca
     5521 ggaagcggat gctggcttcc gctggcgagc tgcagaaggg gaacgagctg gctctgccgt
     5581 ccaagtatgt gaacttcctc tacctggcct cccactacga gaagctcaag ggcagccccg
     5641 aggacaacga gcagaagcag ctgttcgtcg agcagcacaa gcattacctc gacgagatca
     5701 ttgagcagat ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat ctggataagg
     5761 tcctctccgc gtacaacaag caccgcgaca agccaatcag ggagcaggct gagaatatca
     5821 ttcatctctt caccctgacg aacctcggcg cccctgctgc tttcaagtac ttcgacacaa
     5881 ctatcgatcg caagaggtac acaagcacta aggaggtcct ggacgcgacc ctcatccacc
     5941 agtcgattac cggcctctac gagacgcgca tcgacctgtc tcagctcggg ggcgacaagc
     6001 ggccagcggc gacgaagaag gcggggcagg cgaagaagaa gaagtgagct cagagctttc
     6061 gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt cattgcgcac
     6121 acaccagaat cctactgagt ttgagtatta tggcattggg aaaactgttt ttcttgtacc
     6181 atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa
     6241 tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa
     6301 tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca
     6361 aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat
     6421 gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac aaatatattt
     6481 tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg tgttttagac
     6541 atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta atcattgctt
     6601 tataattata gttatactca tggatttgta gttgagtatg aaaatatttt ttaatgcatt
     6661 ttatgacttg ccaattgatt gacaacgagc tccctaggcc tgttatccct aacaagtttg
     6721 tacaaaaaag caggctccgc ggccgccttt ttttcttctt cttcgttcat acagtttttt
     6781 tttgtttatc agcttacatt ttcttgaacc gtagctttcg ttttcttctt tttaactttc
     6841 cattcggagt ttttgtatct tgtttcatag tttgtcccag gattagaatg attaggcatc
     6901 gaaccttcaa gaatttgatt gaataaaaca tcttcattct taagatatga agataatctt
     6961 caaaaggccc ctgggaatct gaaagaagag aagcaggccc atttatatgg gaaagaacaa
     7021 tagtatttct tatataggcc catttaagtt gaaaacaatc ttcaaaagtc ccacatcgct
     7081 tagataagaa aacgaagctg agtttatata cagctagagt cgaagtagtg attggcgtac
     7141 caatcggagc tagggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     7201 caacttgaaa aagtggcacc gagtcggtgc tttttttggc aaaaattttc agattttttc
     7261 ttcatctgta gatttctggg tttttttttc cgtttcgtga atcataagtg aagttttgga
     7321 tgcaaatctg cgcgaaaaaa gttggacctg caatgagctt atttagatag ctaagacaaa
     7381 gtgattggtc cgttgtttca gttctgattg tcagagagtt tgtttcgagt cggcgacacc
     7441 aatgcgtttt gttaaccaga tttcgggtaa gaaatgtatc gagagtttgt ttccagacgg
     7501 ctacatcatt ttcttatgaa gggtgaaatt agatagacca aagattgaaa cacaacattt
     7561 ctttcacaaa aatataataa acttgatagc atttaggatc agccatgggc gcgccgaccc
     7621 agctttcttg tacaaagtgg ttcgataatt ccgatccagc ctaggcccgg gcctgaggac
     7681 gcgtccatgg ttaattaaga cgtccggacc gactagtgga tcctctagag tcgacctgca
     7741 ggcatgcaag cttgtttggg gatctagtgt tttactcctc atattaactt cggtcattag
     7801 aggccacgat ttgacacatt tttactcaaa acaaaatgtt tgcatatctc ttataatttc
     7861 aaattcaaca cacaacaaat aagagaaaaa acaaataata ttaatttgag aatgaacaaa
     7921 aggaccatat cattcattaa ctcttctcca tccatttcca tttcacagtt cgatagcgaa
     7981 aaccgaataa aaaacacagt aaattacaag cacaacaaat ggtacaagaa aaacagtttt
     8041 cccaatgcca taatactcga acaagctagc ttactcagtt aggtcttatc tttaatcata
     8101 ttccatagtc cataccatag cacatacagt agttatatgc tggagaagag atccaacaaa
     8161 acattcacaa tggattatag aaacatttgt ttattcatta taatgagatg ttacattcat
     8221 ttaatattag aaaaagccac aaattcataa cacaacaagc caagaaaaaa acacaaactt
     8281 aagcacacaa cctttttatt tgacacacca aatatttcat cttcatcttc atattcacta
     8341 tcactggagg cttggctcaa gtttggcctg catgacggcg tcgaggaaat cgtcgatcgc
     8401 cttcacggag gagcccttca gctctttttc ctcgttcacg gcgtccttga gctttttgcc
     8461 catttccacg gcgcgattct tcatctccca gcctttggag ccctcgttgc gctcaaggat
     8521 catctcgacg atgcgcttga ccttctcttt ggagatctcg ccttcgaggc ctctagcaag
     8581 ctcaaccgcg acgcccatct catcttcgag gtacttgagg ttgtaggcct gctcggcagc
     8641 aagtggccat ccaagcatcg gaacgccctc gcgaagagac tcaaggatgc tgttccatcc
     8701 gcagtgggtg agaaagccgc cgatggattc atggttgagg atctcaagct gcgggccgag
     8761 cttgtacacg agcttgccct gtttcttcac cttcatgcgt tcctcaaagc cctctggcag
     8821 ccactctggg cgcatctctt cgttgatgtc gaagccgaac ggggctctga tcacccagag
     8881 gaatggcttc tcggaggact caaggccagc agcgagttcc atcatctgcg tcgggctgat
     8941 cgtgttctgg gagccgaagg agatgtagag gacggagtcc ggctctttga ggctgagcca
     9001 ctgcacgaac tcggcgcctg ttgaattgtt gtcggaggag gagtgctgca ctggggaagc
     9061 aataagtggg ccgatgcccc agatcggcag cttggtgtag ttgcggagga tggagaagcc
     9121 gagtgtctcg atctcttcca cggagttgca gagccagcca aaagacttca tggactggcg
     9181 gagctgcggc tggaaatact tggaccaatc gtcggagccg tcggcatatc tgaggaaccg
     9241 atggagctgg gacctgcgga acttatggtt ctctgggaag cctggcagcg ggaactcttg
     9301 gtcgtcggag tagttctggt gcgggagatc gttccagatg gacacgtagg cggatgtgcc
     9361 ataagcgccg cctgttgtga acaccacgcc tgtagagccc acatccttgg ccacctggtc
     9421 aacccagccg aggaacacat catggatcac gcagagtggt ggcctggctg ggctgaaatg
     9481 cctggagatg taatcgcgga ggtgcggatc gaggctgatg gtggaatgga agagggacac
     9541 cacgagcggg agtgtgagct tatcggtgtt ctcgatgccc ggtgggagtc cgtggttggt
     9601 ggagttgaat ggcagctcga cgatcctgat gccgctggag gagtaggaat ggtggtgaag
     9661 gaggtgcctg aggaagccgg cattgagcgg agttgtgagc agggtgatga tcacgtggga
     9721 gcgcttgtag aggaacatgg cgagttcgag aaatggcctg aggtggccct gcgccatgaa
     9781 cgggatcatg aggatgtgct gtgtctcgcc ctcgccgttg gtgttcatct tgatggcggt
     9841 catggggcca ggattttcct caacatctcc tgcttgcttg agcaggctaa agttggttgc
     9901 tccggatccg gcggaggtga acttgtagga gccgtggcaa agtgtgccat gatcccagga
     9961 ggagtggatc agctcggcct tccacttttc accagcagcg ccgagcacaa cgtggagcgg
    10021 gtagaagtgc tctggaaatg gatgggcgag cttccagttc ggggccttgc tctcgtagat
    10081 gttcacttcc tcgaagcggc cgttgatgag ggccttccta agccaagaat cgaaggcagc
    10141 ggcccaaggg gcaacaccat cgaagtagtg cggtgtctca tcgagtggat gtgtggcgct
    10201 gccggagcca ataatgagca cgccatcatt cttgagcggc gcgagggctc tgccgagatt
    10261 gtagtggtag gtgccgtcga gatgtggctg aactgagagc tggcacaccg ggatgtccgc
    10321 ctctggatac atgagcatga gtggaaccca ggcgccgtga tcaaggcccc tcttttggtc
    10381 tgtctcggct gtctcgaacc cggacttctt gaggatctct tccacctttc tcgcaagctc
    10441 tggctcgcct ggagctgggt acttgaactg gtacatggcg gctgggtagt cgtcgaagtc
    10501 gtggatggtg tcgttgatgt gcacggcgtt cacggttggc ttcactgtct cccagtggcc
    10561 ggagatgatg aggatggcct tcggcttctt ggagaaaatc ttctcgcgcc atgtctcgaa
    10621 gaacggcctg agcggatgtg tatcctcgac ggtgaggatc gggttgccgt gggtgatgaa
    10681 gaaggactct ttgatcatct ggtcgttggc gtcctcgccg ttcatcatct tcataggccc
    10741 ggggttctct tctacatcac ctgcctgctt aaggaggcta aaattggtag ctccgctacc
    10801 gtagcgcgga atcgggatga gcttgagcgg cttggtcttt tggagcgcaa tgccgaactt
    10861 ctcgtccatg tcgaggtcct tcggggagat gtcgccttcg agcttccagt tgaagaactg
    10921 gaggagtgtg gcgagcatga gggtgagcat cctgatggcg agattcatgc ctgggcaaat
    10981 tctgcggcca gcgccaaatg gaaggaggcc gaaatcgcgg cccttaacat cgatctcgca
    11041 gccgatgaag cgctctgggc tgaaaatatc ggcgttctgc caggcgtttg ggtccctgcc
    11101 aatggcccag aggttcacga ggatctgggc atccttcggc acgatgtagc cgtacagctc
    11161 gacatcggtg tcggcctttc ttgggagcaa gaacacggtc ggcggatgga ggcggagtgt
    11221 ctctttgatg atcgcctgga ggtacggcag gttgatgatg tcggactctt ggatctgctt
    11281 gtccttgccg aggacctgct tgatttcctc ttgggccttt tccatcatct ctggattgcg
    11341 gatcagctcg gtcatcaccc actcgaatgt ggaggatgtg gtatcggtgc cggcgtcgaa
    11401 gatgtccacg aggaggtggt tgatctcgcc catcgtcagc tcgttctgct tgaacagctg
    11461 gaggagcaca tcgagcacgt cgtcggtggt ggtggtagtt gtggtggagg aatctggggc
    11521 gagtctctcg cagatgatgc cctggaacac ggcgatcagc ttgtcgaagg agcaggcgag
    11581 ccttcttcta atgccagatg ggtcaacgca gccgagaatc gggaagtagt cggcgtagtt
    11641 cggcttgcct atatcttcca tgatgttcca gatcagctct ttgaactctt ggctggtatg
    11701 ggacttgtgg tgcgccagct cgacgctgaa gaacagcttg ctgaggaggt tgaggctggt
    11761 cgtaaaggcg gccttgccaa tatccacggc ctggcctttc tgggcgcact cttgaacgta
    11821 ctcgtagagc tgctgcacct tggcgtgcct gaatgtctgg caggcatcga gtctctgtgg
    11881 ggagagcaga tgcacggcgg taatcttgcg gaagttgcgc cacttcggag acaccgggag
    11941 ccaagacatg gtgagcttgt ggtggtcgcc ggctgtaaca ctattcggga tcgtgcggtt
    12001 ggagagcggg tgatccttct tgaggaacat ctctttggcc acgtcggcag aggacacaac
    12061 gatggtggtc acagagccga gtctgaggga aatgagtggg ccgtgaatct tggcgaggtt
    12121 ggcgaaggac cgatgcggct tcttgcccac ctcaaggatg ttgccgatga tcggaagcgg
    12181 ctttggtcct ggcggaagca gcttggtggt ctgctgggag aacagcagct tgatgaagtg
    12241 gaagctgatg aaccagatcg cgaggatcat ggcgagggtc gcatgatcca tactagtttt
    12301 cagcgtgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga
    12361 tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt
    12421 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
    12481 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
    12541 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
    12601 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
    12661 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
    12721 atgttcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
    12781 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
    12841 agcctttcct tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc
    12901 ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
    12961 tttgttgaaa agtctcaaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
    13021 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
    13081 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttaa
    13141 agtactttaa agtactttaa agtactttga tccaacccct ccgctgctat agtgcagtcg
    13201 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
    13261 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
    13321 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
    13381 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
    13441 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
    13501 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
    13561 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
    13621 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
    13681 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
    13741 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
    13801 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
    13861 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
    13921 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
    13981 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
    14041 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
    14101 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg
    14161 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
    14221 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
    14281 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
    14341 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
    14401 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
    14461 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
    14521 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
    14581 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
    14641 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
    14701 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
    14761 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
    14821 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
    14881 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
    14941 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
    15001 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
    15061 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
    15121 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
    15181 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
    15241 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
    15301 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
    15361 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
    15421 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
    15481 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
    15541 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
    15601 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
    15661 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
    15721 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
    15781 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
    15841 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
    15901 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
    15961 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
    16021 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
    16081 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
    16141 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
    16201 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
    16261 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
    16321 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
    16381 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
    16441 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
    16501 aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca
    16561 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
    16621 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
    16681 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
    16741 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
    16801 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
    16861 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
    16921 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
    16981 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    17041 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
    17101 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
    17161 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    17221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    17281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    17341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    17401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    17461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    17521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    17581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    17641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    17701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    17761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    17821 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    17881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    17941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    18001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    18061 ttttggtcat gcatgatata tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa
    18121 taataaaagc agacttgacc tgatagtttg gctgtgagca attatgtgct tagtgcatct
    18181 aacgcttgag ttaagccgcg ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac
    18241 attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact
    18301 gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa
    18361 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
    18421 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat
    18481 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg
    18541 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca
    18601 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg
    18661 ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc
    18721 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag
    18781 cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag
    18841 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
    18901 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
    18961 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
    19021 ccgcttcccc catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt
    19081 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
    19141 gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
    19201 cgttaccacc gctgcgttcg gtcaaggttc tggac
//
