LOCUS       sequence-448022-        3811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro synthesis of mRNA for fluorescent protein mScarlet3 
            (control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Karapidaki I, Handberg-Thorsager M, Momose T, Yasuo H, Genikhovich 
            G, Assaf S, Deleau C, Pang Y, Clayton P, Lohmann B, Rusciano ML, 
            Stranges M, Mathieu J, Zilliox M, Ustyantsev K, Salmon B, 
            Laplace-Builhe B, Koenig M, Colgren JJ, Arnone MI, Berezikov E, 
            Brunet T, Bucher G, Burkhardt P, Dickinson DJ, Houliston E, Huisken 
            J, Leclere L, Averof M
  TITLE     A toolkit for testing membrane-localising tags across species
  JOURNAL   bioRxiv 2024.11.12.623055
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.12.623055"
COMMENT     Sequence Label: 0_T7-mScarlet3
FEATURES             Location/Qualifiers
     source          1..3811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(789..805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(789..805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(802..824)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(977..1565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1057..1076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(1682..1706)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(1806..2597,2598..2666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2429..2448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2667..2771)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2870..2892
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2884..2901
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2885..2901
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2954..2973
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2954..2972
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3040..3061
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3106..3792
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     3504..3523
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttataagttc tgaaccgaga aagaggggag aatgtagagc cgatagctgc ccaatactgc
       61 cacgccccca gcagcccagg catggaaaat aatacagaga aaatccacac ttattaggtg
      121 ctactgtaat tttttttaaa tcgcttaaaa ttattcggcg tttgcctagt gcaaggccca
      181 tttatggaca attgtttgcc aaactcaaag tttgccggtc ggaggaaaag ttacaacgcc
      241 aaaatgtgtt gaaaaatgca aaagaaatta ttaaaaacat taaaaaatta ttaaataatc
      301 tatgtaattc ttgaatgtaa actctgtgta aatactaaaa atgaaaaaca aaatggaaag
      361 cgtagatgga gaacagttga atcgttatat atttaaatta atattgcatt tatttccaaa
      421 ttacagtggg agaagcgctg accgacttct tgcagtgtac tctcgaaata aaacccctgt
      481 ctgtaccacg taaaacccct actaaaaatt agtctcgccc gctgctcaca gttagtttag
      541 ttcaattata cgctactgtt taatttatta actgtactta ttaatgcgcg tcatgaataa
      601 tgattgactg tcccttattt tttttttttg ctaatttctg tattaaattt aatgatttac
      661 cataaaaata taaataaaaa tgacggtcga gttgtaaaat taataaaaaa aaaaaacgaa
      721 tgctgcggcc gcaggctagg tggaggctca gtgatgataa gtctgcgatg gtggatgcat
      781 gtgtcatggt catagctgtt tcctgtgtga aattgttatc cgctcagagg gcacaatcct
      841 attccgcgct atccgacaat ctccaagaca ttaggtggag ttcagttcgg cgtatggcat
      901 atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      961 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1381 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tctattcaac aaagccgccg
     1621 tcccgtcaag tcagcgtaaa tgggtagggg gcttcaaatc gtcctcgtga taccaattcg
     1681 gagcctgctt ttttgtacaa acttgttgat aatggcaatt caaggatctt cacctagatc
     1741 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1801 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1861 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1921 ggccccagtg ctgcaatgat accgcgagag ccacgctcac cggctccaga tttatcagca
     1981 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2041 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2101 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2161 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2221 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2281 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2341 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2401 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2461 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2521 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2581 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2641 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     2701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2761 ggggttccgc gcacatttcc ccgaaaagtg ccagatacct gaaacaaaac ccatcgtacg
     2821 gccaaggaag tctccaataa ctgtgatcca ccacaagcgc cagggttttc ccagtcacga
     2881 cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa ggaagtgcca
     2941 ttccgcctga ccttaatacg actcactata gggcgaattg ggtaaagtgc aagttaaagt
     3001 gaatcaatta aaagtaacca gcaaccaagt aaatcaactg caactactga aatctgccaa
     3061 gaagtaatta ttgaatacaa gaagagaact ctgggcgcca caaccatgga tagcaccgag
     3121 gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat gaacggccac
     3181 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     3241 ctgagggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc ccctcagttc
     3301 atgtacggct ccagggcctt cacgaagcac cccgccgaca tccccgacta ctggaagcag
     3361 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgctgtg
     3421 tccgtggccc aggacacctc cctggaggac ggcaccctga tctacaaggt gaagctccgc
     3481 ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg ctgggaagca
     3541 tccaccgagc ggttgtaccc cgaggacgtc gtgctgaagg gcgacattaa gatggccctg
     3601 cgcctgaagg acggcggtcg ctacctggcg gacttcaaga ccacctacag ggccaagaag
     3661 cccgtgcaga tgcccggcgc tttcaacatc gaccgcaagt tggacatcac atcccacaac
     3721 gaggactaca ccgtggtgga acagtacgaa cgctccgtgg cccgccactc caccggcggc
     3781 tccggtggct cctaagcgat cgctatcgat g
//
