LOCUS       sequence-44803-e        1143 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Dieriks B, Van Oostveldt P
  TITLE     Spatiotemporal behavior of nuclear cyclophilin B indicates a role in
            RNA transcription.
  JOURNAL   Int J Mol Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937.
  PUBMED    22426501
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937."
COMMENT     Sequence Label: PcDNA-DEST47-CypB-Del2 C-term GFP
FEATURES             Location/Qualifiers
     source          1..1143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(543..642)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(659..678)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(660..678)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(683..699)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(683..699)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(801..820)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(880..899)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 actaagtgag ggggcaagtc accgtcaggt gtattttgac ctacgaattg gagatgaaga
       61 tgtaggccgg gtgatctttg gtctcttcgg aaagactgtt ccaaaaacag tggataattt
      121 tgtggcctta gctacaggag agaaaggatt tggctacaaa aacagcaaat tccatcgtgt
      181 aatcaaggac ttcatgatcc agggcggaga cttcaccagg ggagatggca caggaggaaa
      241 gagcatctac ggtgagcgct tccccgatga gaacttcaaa ctgaagcact acgggcctgg
      301 ctgggtgagc atggccaacg caggcaaaga caccaacggc tcccagttct tcatcacgac
      361 agtcaagaca gcctggctag atggcaagca tgtggtgttt ggcaaagttc tagagggcat
      421 ggaggtggtg cggaaggtgg agagcaccaa gacagacagc cgggataaac ccctgaagga
      481 tgtgatcatc gcagactgcg gcaagatcga ggtggagaag ccctttgcca tcgccaagga
      541 gcacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
      601 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
      661 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
      721 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
      781 tgcttacata aacagtaata caaggggtgt tatgagccat tattcaacgg gaaacgtcga
      841 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      901 atgtcgggca atcagtgcga caatctatcg ctttgtatgg gaaggcccga tgcgccagta
      961 gttgttttct gaaacatggc aaaaggtagc gttgcccaat ggattgttaa cagatgagaa
     1021 tggtcacaac taactggcct gacgaatttt atggccctct tacgaccatc agacatttta
     1081 tccgtacgtc ctgatgatgc catggttact ctcaccacct gacgaatccc cagaaagaac
     1141 tgt
//
