LOCUS       sequence-448145-       10906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10906)
  AUTHORS   Manning BD, Tee AR, Logsdon MN, Blenis J, Cantley LC
  TITLE     Identification of the tuberous sclerosis complex-2 tumor suppressor 
            gene product tuberin as a target of the phosphoinositide 
            3-kinase/akt pathway.
  JOURNAL   Mol Cell. 2002 Jul . 10(1):151-62.
  PUBMED    12150915
REFERENCE   2  (bases 1 to 10906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2002 Jul . 10(1):151-62."
COMMENT     Sequence Label: pcDNA3 Flag TSC2
FEATURES             Location/Qualifiers
     source          1..10906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(264..287)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      287..296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(310..329)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(311..329)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(374..577)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(403..423)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        578..957
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1129..1148
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1223..1327
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1328..1396,1397..2188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1546..1565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2359..2947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2848..2867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3101..3118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3235..3256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3271..3276,3277..3294,3295..3301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3309..3325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3314..3336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3333..3349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3333..3349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3398..3519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3409..3428)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3463..3482
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3693..4487)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3804..3823)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4414..4433
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4554..4883)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4568..4703
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4622..4641)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4868..4888
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4897..5325)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5007..5028)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5219..5238
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5371..5595
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5584..5601
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        5621..5639
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5621..5638
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     5649..5665
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5649..5665
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 accgccttcc agagtgcttc cactgcgtgc tcttcaaatt tcttggtttt tgcgacttca
       61 caaatctgcc ctatcatccg gatgcgattg ttgaggccac attccatgct cagttctctc
      121 agtatttccg cggtgatgat aaactccgtc tgtttaccct ctgcagacct gggatttggc
      181 ctcggtgttc ccagtcccaa cagaatctta aacttctcct tcaagcctga atctttgctt
      241 gttggtttgg ctggatccct tgctttatcg tcatcgtctt tgtagtccat ggtggctaag
      301 cttgggtctc cctatagtga gtcgtattaa tttcgataag ccagtaagca gtgggttctc
      361 tagttagcca gagagctctg cttatataga cctcccaccg tacacgccta ccgcccattt
      421 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
      481 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
      541 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
      601 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
      661 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
      721 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
      781 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
      841 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
      901 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaac
      961 gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta agcagattct
     1021 tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta aattttgctc gcgcactact
     1081 cagcgacctc caacacacaa gcagggagca gatactggct taactatgcg gcatcagagc
     1141 agattgtact gagagtgcac cataggggat cgggagatct cccgatccgt cgacgtcagg
     1201 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1261 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1321 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1381 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1441 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1501 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     1561 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1621 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1681 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1741 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1801 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1861 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1921 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     1981 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2041 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2101 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2161 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2221 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2281 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2341 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2401 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2461 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2521 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2581 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2641 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2701 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2761 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2821 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2881 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2941 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3001 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3061 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     3241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     3301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     3361 caagctctag ctagaggtcg acggtataca gacatgataa gatacattga tgagtttgga
     3421 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     3481 gctttatttg taaccattat aagctgcaat aaacaagttg gggtgggcga agaactccag
     3541 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     3601 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     3661 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     3721 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     3781 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     3841 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     3901 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc
     3961 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     4021 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     4081 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     4141 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     4201 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     4261 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg
     4321 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     4381 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     4441 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     4501 catcctgtct cttgatcaga tccgaaaatg gatatacaag ctcccgggag ctttttgcaa
     4561 aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggcagaggcg
     4621 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     4681 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
     4741 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
     4801 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     4861 tttccacacc ctaactgaca cacattccac agaattaatt cgcgttaaat ttttgttaaa
     4921 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4981 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     5041 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     5101 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     5161 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     5221 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     5281 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtgggga taccccctag
     5341 agccccagct ggttctttcc gcctcagaag ccatagagcc caccgcatcc ccagcatgcc
     5401 tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag
     5461 aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg
     5521 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
     5581 aactagaagg cacagtcgag gctgatcagc gagctctagc atttaggtga cactatagaa
     5641 tagggccctc tagaactagt ggatcccccg ggctgcagga attccttttt tttttttttt
     5701 tttttttttt tgtgcctcta tgtctgtgca ctggggtcag gactttattt atttcactga
     5761 caggcaatac cgtccaaggc cagtgcagga gggagggccc cggcctcaca caaactcggt
     5821 gaagtcctcc accgaggaga tgaggcgctt ccgctggccc acctcatagc caggtgtggg
     5881 ctcggctgga gtctgtgcag gggctttgct atgggacgga gggtgcacca gaggtaggct
     5941 ggggttggag taggcggctt cctcgcagat ccgctggcgg agccgcttga tgtggcggag
     6001 ccgggcaatc cacttggagg ggtagatatc ggtggggttg gagcggctat gatgcacctg
     6061 tgaggccata tttgcgtgca gggccatctg gcgggccacg aagggcaggt tgcggtcaga
     6121 cacgatcttg gccacgctgg tgtccacaag gccctccatg tctttcctgc actgcaggga
     6181 caccaggttg cactcgtagt ccagcggggt gacgatcacg tggacaaagt tgaactggcc
     6241 cttgatggtg ccaagcttga agtcctcacc ggagtcattg tagacaatgg acacaaagtc
     6301 gttgcccagg tggcgcttct tgtcgcagcg gtgcttgtcc acgtccttgg tgggcatcag
     6361 ggtggcgatg tggaagacgg cttgcatgat gtcatcgtgc cagcagtagg tgaactggcc
     6421 gtcctcacca cacacgtcca ggcctcccag gtacaccttg tccggctggc agtccttcag
     6481 ctcgatgagc cggcccaggc ccgtcaggaa ctccgtgtac ctgtaggagc catgctcatt
     6541 ggacaggatg gcgagctcgc tgttgctctg gccttctcca acatacagga cggcgatctt
     6601 gtgggtgtcg tatgatggga tctggtcgag gagctgcacc gaccgctcaa aggactgtga
     6661 ctcattgggc agcaggattg gcttgtttga ctcgtcgcca aagaaggggg aatggtagag
     6721 ctgcaggaac acgaaactgg ggttgatgcc tggcactttc tctgcattgg aggctgtggc
     6781 tctgctcttt aaggcgtccc tctctactct cttgcccctg cgtgatgggg ccgagtcgga
     6841 gatggtgtaa cctcggggcc ggaggccact gggcgagcgg ggggagctgg aaggcaaggg
     6901 accctcgggc tggccccgac tgtcttcgcc cgaggccgac caggcagcac tttccccgtc
     6961 cagggtccct gactgtgacc gggccttaac ctcagggctc agccggccca cgtcggcctt
     7021 gtccccaggg tccccgagga tgtcctgcag agtctgcagc tcgggagagg agctggactt
     7081 gctcaggggc tgcgagggct ggaaggagag gtccacagag ggccggccac cctccgagga
     7141 gacgactcgc tcgatgggga tgcccctgcc aaccagctcc tccgcgtgga gcgacttctc
     7201 ctcctggctg gagactgagg acgacctgct gtaggcatcc gtgcgcctgt ccatgcctag
     7261 cgctgcctca acgtcctcca accctggggg ctccaccagc acaggaacct cgcccggact
     7321 tccctcctcc atgaccacgg cggagtctgt gttggagcga ggcagaggag ggggtttggc
     7381 cgtgctggct gccggcaccg acagtgactt gtacagggct gtgtcccggt gctccttgaa
     7441 gcgctcagcc gccatgaggg cgttagacag ctcctgcagg ggcatgttgt tgatgtccga
     7501 ggagaaaggg ctgagcgggt tctccaggct catcagccag ctggtgttcc ctgtgggcct
     7561 ccggaccagg atctccgccc agccctgggt cagcaggggc acataggccg ccaggttcgt
     7621 cttctcctgc acaggaaccc gggtgccagc tgaggccttc tcaggtttcg cggctggtgc
     7681 agtccgtggt cctggagaag tggcactgcc caggaactgg gaggccggca cgtccagggc
     7741 gccaactcga agaccatggc cccccgacat ggaacggacc cgatcccggg ccccacggga
     7801 cacctgctgc ccagcctggg actccagctt ggccggcgcc tccttggtct gtcttacatg
     7861 caccccgggg ctggagctcg actccgggcc ggactgcagc tcccccgagt ccaggcctag
     7921 taacgaccgg gtcccggttc ccacgcttgt cgtcacagtg acaagcttgt tcccaaccag
     7981 ccaggttttg gtcctgccac ccgctaggag gaactcgccc acaggagacc tcttcgggac
     8041 agccgtgaag ttggagaaga cgtatcgagc catcatgtcc agacaggttt ccgtgagctc
     8101 caggtggagg tttttcaggc tatcgtcagc ctgggccacg gagttctcat ctgcagaccc
     8161 caagctggca ctggtgaggg acgtctgtat cctgctgcgg accacatgtt cagacacact
     8221 gatgctgcgg caccggaagg cctcggctgc agagctctcc ttgaattctt tcacgggtgg
     8281 agagttattc aagccttgtt tggggggtct ggctatcctc agactcttgg gtctctcgtt
     8341 gagactagta ctccgggccc tgaagctgtc cttctcgggg gtgtcatcaa aagacaagag
     8401 gacattggac cgcaggccct tagtgatgaa agggacaaaa tccttccgga agggcaggcg
     8461 gcacctgatg aaccacatgg ctatgacgtg atgggccaga cacacgatgt actgattaaa
     8521 cttggagggg ttggtgtacg gcagggagat ggcgaacaca ctggcatact gctccgcggc
     8581 aaagttcctg tagaggtgcg gcagcctggc cagagtggac aggaactcca gcagtgggac
     8641 ggccatgctg gctgtggctg agatgtgcgt gagcttcacc accagaacag gcagcgcctt
     8701 gatgatgatg tcaggcatct ccacgctgca gatggacaag gccacgacgc actggctggc
     8761 acagcggtgg atgaggccct gctccaggca gtagaccatc tcgcgctgtt tggttttgtc
     8821 caggtagtta tggtaagaga ttaatgctgt cagcactgga accacggcca ggtgcaagtc
     8881 agttctggag aagccttctg gggcgcctcg gagccgctcc agtgtctttg ggcctgaaag
     8941 catggagcag agagcagagc acagctggtc cacactgcaa ggggaagtaa agatgagcac
     9001 tttatagcgc agggactcag gcagcctgcc cagaaccagc ttcagcacct tccagtcaga
     9061 ctcctgcttc aagcactgca gcaggacgcg gaagagcagg gagtagggca cggaccccag
     9121 ccgcacggcg gggcctgcag gcgccgggcc aggaggccct gtgggaggag aaagggggcc
     9181 gctggtcttc ttctcagagc ctctctctgg ctccatgtag tcgcagacgc agtaggggct
     9241 gaaccgcacg actccatcct tgttgggcag gcccaggcgg tgcagtgagt cggcccgcag
     9301 cagcaacagg aagtcaaagg cctgcagccg gatgctgctc gcgattggca gggtgtagct
     9361 gtgcttgtag tggagctgaa tgtggctgac cagcatctca tacacacgcg tggcgtggct
     9421 tgcaggcagg gtgtacagct tggtctgaag gatgaccaga agccccagga cggctgtctt
     9481 cacatcctcc aaggaggccg agtatgcggc cacatccctt tcttccagct ccgggggtgg
     9541 ggagagggag cgggccatca ccttctcgat gatgtccagc aggctgttga agtggtgtgt
     9601 gtggcagccc tctgccaggt ccaccagcaa ctgggtggcc agctttcgga cctggtggtc
     9661 tttatcctcg gggatgtggg agagctgcga gatgaccact gagttaatca gctcctcctc
     9721 atagaactgc ctgttgatga gcagcacaaa ggacagcacg tccagcacct tgatgcgcac
     9781 ggcgcctcgg gactcgctcc tgaagaatct ctccatcagc gcctgcaggt tctgaatcca
     9841 gccgtccttg gccgggtgga tggactgcgc tctataggag atcaggttca ggagggagga
     9901 ctcaggcctc tggtccgcac atctctccac cagttcaaag tatctctcct gagacccgtg
     9961 gaactcgttc tggtcacaca gctcctccac cgtggtcaac aggtcatgga cgatggtcct
    10021 gagctccggg ctgtccaagg tctggagctg ctgaaggagc cgttcgatga tgttcagcag
    10081 aatgtcccac gccaccacct ggagctcctt cctatacttc ttgatgagcc tggtgatgga
    10141 caggacgatc tcataggaca ccacctcgtt cggacatgcc atggcctggt aaaatgatgg
    10201 caacacagat gtcggcgagt tcctgagaga atagagccgg tgggctcccc agagagccat
    10261 gcccacaaaa aacacggctc ctctcagcag gggcgcgtcc tccatgtagg ctctgtcctc
    10321 catgaggtgg cacatgttgt agatggcgct gtggcccagg tgggtgccaa ggaggttccg
    10381 catcagcttc cagcaaggct cgcagagctc cttgacgttg atggtgcgac agagggtaac
    10441 gatgaacagc gggaggctct cagccggcag gcagttgtag cagaccacgg cgtccagcac
    10501 ctgcagggag acctctatgt ccacagagga cgcggtccgg acgcacagca gacagatcat
    10561 ctgaaccatc cttgcgatgt actcgtcgag gtaacagcta ttgaatttga ccaagttcac
    10621 cagcaccaga aggaattccg aggacaagcc aacatccatc cactgcagga caaagtcagc
    10681 cagctcttcc tccaagtagg tgatgtgtct cccattgtct gtgagggcct tgaaaacctc
    10741 cagcctttcg tgaaggtctt cgttggaagg gtaatccttg atgaccttaa agaagagggc
    10801 tctgaggacc cccaaacgct cgccctgccc ctgcacgatg gccttcagca gagccagcac
    10861 cgcgtgccgg gcctccagcg gccgctccgg ctgcaacaga tccgcg
//
