LOCUS       sequence-44816-e        1183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Dieriks B, Van Oostveldt P
  TITLE     Spatiotemporal behavior of nuclear cyclophilin B indicates a role in
            RNA transcription.
  JOURNAL   Int J Mol Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937.
  PUBMED    22426501
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Med. 2012 Mar 13. doi: 10.3892/ijmm.2012.937."
COMMENT     Sequence Label: PcDNA-DEST47-CypB-Nucl2 C-term GFP
FEATURES             Location/Qualifiers
     source          1..1183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(543..642)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(659..678)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(660..678)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(683..699)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(683..699)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(880..899)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cattaaagga aatgagagag aggggcccaa agtcaccgtc aaggtgtatt ttgacctacg
       61 aattggagat gaagatgtag gccgggtgat ctttggtctc ttcggaaaga ctgttccaaa
      121 aacagtggat aattttgtgg ccttagctac aggagagaaa ggatttggct acaaaaacag
      181 caaattccat cgtgtaatca aggacttcat gatccagggc ggagacttca ccaggggaga
      241 tggcacagga ggaaagagca tctacggtga gcgcttcccc gatgagaact tcaaactgaa
      301 gcactacggg cctggctggg tgagcatggc caacgcaggc aaagacacca acggctccca
      361 gttcttcatc acgacagtca agacagcctg gctagatggc aagcatgtgg tgtttggcaa
      421 agttctagag ggcatggagg tggtgcggaa ggtggagagc accaagacag acagccggga
      481 taaacccctg aaggatgtga tcatcgcaga ctgcggcaag atcgaggtgg agaagccctt
      541 tcacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
      601 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
      661 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
      721 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
      781 tgcttacata aacagtaata caagggggtg ttatgagcca tattcaacgg gaaacgtcga
      841 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      901 atgtcgggca atcagtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
      961 gttttctgaa acatgggcaa aggtagccgt tgctcgattg atgtttacag atgagaatgg
     1021 atcagactaa aactggctga cggaatttat gcctccatcg gacatcgaag caattttatc
     1081 cgtactcctg atgatgccat gcctactcca cccactgcga ttccggcaaa acaagcattc
     1141 caagtattaa aacaatatcc tggaattcag ggaaaaatat atc
//
