LOCUS       sequence-448178-       17974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  D. rerio elavl3 promoter driving expression of human glucagon 
            receptor, TEV protease recognition site, QF fusion protein for use 
            in trans-Tango.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17974)
  AUTHORS   Coomer CE, Naumova D, Talay M, Zolyomi B, Snell NJ, Sorkac A, 
            Chanchu JM, Cheng J, Roman I, Li J, Robson D, McLean DL, Barnea G, 
            Halpern ME
  TITLE     Transsynaptic labeling and transcriptional control of zebrafish 
            neural circuits.
  JOURNAL   Nat Neurosci. 2025 Jan;28(1):189-200. doi: 
            10.1038/s41593-024-01815-z. Epub 2024 Dec 19.
  PUBMED    39702668
REFERENCE   2  (bases 1 to 17974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-024-01815-z"; journalName: "Nat Neurosci"; date: 
            "2025-01"; volume: "28"; issue: "1"; pages: "189-200"
COMMENT     Sequence Label: elavl3:hGCGR-TEVcs-QF;he1.1:CFP
FEATURES             Location/Qualifiers
     source          1..17974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(24..35,36..198)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    714..738
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9445..9469)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10918..10938
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    13426..13560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13450..13469)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     13504..13523
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      14183..14192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(14189..14191,14192..14194,14195..14908)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKLDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(14234..14255)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(14495..14514)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     14842..14863
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(15271..15288)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    15277..15502
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    join(15831..16018,16019..16030)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(16056..16073)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16227..16815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16307..16326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16986..17777,17778..17846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17609..17628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17847..17951)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtatctgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt
       61 gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat ctggctagaa
      121 tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt attaaggaag
      181 taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa tctcaaaaaa
      241 cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta tttaattatc
      301 aaactataag acagacaatc taatgccagt acacgctact caaagttgta aaacctcaga
      361 tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc tcttttgggg
      421 ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc gggaagaggt
      481 gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga ctccctggtg
      541 tctgaaacac aggccagatg ggccattcgt acgcttaatt tcgaatatcg atatcaaggg
      601 cccatctcga gtatccggac acctaggtag catgcatggc gccttgtcga ctagctagca
      661 tcccgggaag aattcctcga cggatccacc ggtgtggaat tctgcagata tcaacaagtt
      721 tgtacaaaaa agcaggctct attcctaaag accttgggtg accaaaatct tattttaata
      781 aataaaactg tttattaaaa cttttttgtt tcaaagaacc atatgtatag tgaaatttat
      841 aaaaatatca atttttaaaa agctggtgta ctcatttatg ttatgaactc taaaaccata
      901 tactgactgc aagtgatgat gtatagagtg atgtttacga gtaaacatat ttagttgtat
      961 acatcctact gagcacattt tgatgtatga aataacatta caagctttat ccaaattaag
     1021 ccattttaaa acactgccaa ttgaaaatac aaatcctgga aaaaatcgtc tttagcgcag
     1081 tcatttgagc catcctaatc cgttacctca gaccataata agaagggata acactagctg
     1141 tagcaatgga acacatctgt ttcacacaat catatctcct gcgccggtgc taagcagatt
     1201 cagcgtgatc ataacatgct ttccactcat aaatgtaaat ttacaatttg cacatgtaaa
     1261 acagacactt ttgagatatt ggataaaaaa acaagagtat attgcttagt ttcatccacc
     1321 agtcatcccc acagcgtttg gaaggccata aaaagtgtct aaaatcaatg atcattgaaa
     1381 gagcacaaga gagactctta cgctgtaatg ccactgggga caaaagtgac agtctcttaa
     1441 tgggctcttc tggaggggct cctgaacatt aaaaattatc agcgaaatta ccgaaagagc
     1501 ttcaagcaac tggcatgctt gatcctctgc gtcggggcgg tgaataggtg cttcagatgc
     1561 cctcttaccc acgggctgga ttcagctgcc ccgctaccag cggagacccc ctaatgagcc
     1621 tctgcaatta agtttattca tgttaagtgt gaacggggtg cgtgcggaac tgtgggcagc
     1681 taacagacct gggttctttg tgccacaagt gctgccttta ttcggctcac aaagcagaaa
     1741 acaacacccg cacctataat ggcgccctcg gctgggtcta agaaacgtgg cgagttgaca
     1801 gagcagagtg ggcggggtta agacagactg acagcgggac ccatctccat cctcttatta
     1861 acgcttaacg agtgccttcc tcatgcaata ttcatcgcca ctaatatcat ccaagctctg
     1921 agctgagctg gccacttatg taaggcaatt atgtaaaata tcagacaggg cccacactca
     1981 gaatctgact ggggtagaga cgcgggacga gaaccgagag caagaactga aagtgaaagt
     2041 gaccactaaa gggaggagag gacagagggg caggatgtgt caagattacc agagaacact
     2101 tggccagaaa tgcgcaacca ttggagctct ccggattacc caaaggttaa cgagtttgaa
     2161 cgcctctgcc cactcgccca tctctgatgg tttcccaaga actcctcaag caaaatatat
     2221 ataattgtgt gtattatgca cagacacgag aaaatgctgt ttttctgatc tgcattacag
     2281 cacatttgcc cgccaacgac aataccaccc actcggtacc tcgctgactc ctgatgcctg
     2341 atacctgcgc ggtgactgtc tacaatctgc ataatcaaga gaagttgtgt tgaagacgag
     2401 cgccacacaa ccgtttccac aaggtcaccc aaggccggtg cagatgtagg tgaggtctcc
     2461 ataaacagac tgaaataaac acatcctccg ctgggaacaa caaccccctc acgcctcatg
     2521 catttccata agcctacatg catctcttcc aacttatgga gactcgcacc taccaacatc
     2581 cgcacaacaa agatatacag agcgcgctcc ctcaggtcaa ggccctgtgg gggtctgtgc
     2641 agaaataggt catttgtcac acatcaagtc ctggggcagg agatgcatta tagatgagac
     2701 caaacagcct gtctcggtga gctctaccca ctccctgaga ctagaaatgg gggaagggag
     2761 cttgagataa caaccgctgc aatcactgtg tcgatgttta atatcagcac caaccgggaa
     2821 caataaggag atgggtgcat tcatgttcac atcttaccag tcaagtatca tcgaaccggc
     2881 ttgataacca cacctcgtgt aatagctgag cagatagttg tcattttaaa gcgttggcct
     2941 ttgtcgatta tgtaatgcgc acattcaaca catggtaata tagaaacggt tatgtcgagg
     3001 ttgttttgtc cagagatgac cttcacacag ttacagccgc tctgcatcca cacaaatgga
     3061 ggacttaatc gtggactgca ttcttagaaa tgatctacaa agacaaataa tgtgaaatca
     3121 agaaaggaca aaatttaagt aaggggatga gggagagaga gaacgagggg caaggagaaa
     3181 gcatggctcc tgtctttttc tgcacccatc tgttcggagt gcaggtggag ctctattcac
     3241 tcagctctgc atgtgtgttt ggggggggca ggaagaaagg gagggcaaaa ggaagagtgg
     3301 agagatggtg ggggctggag ggatgggggg ttctcggtga tctctcctga aggggataat
     3361 gggagagcag cgctttgcaa tggctgccat gtagtaccct ccctgcacaa ttagccaatc
     3421 agcagcaagc tctgccagcc agaaggacac ataaaagaag aacattgcag cagaggcaca
     3481 gaaggagcct gcgaggagct gggaaataca cacacaacag cagaaccaca acaccctccc
     3541 ctggacacac cctactgggg atcactgctt ttcttttttt ctgaaccatc gcccacgcca
     3601 cacggagaga aatctctctc tcatcatcat cctgaagaaa acccccttat cctcattttc
     3661 acactgctga ggaaaaccta caatcgcacg ggctgagatt tcctggcgaa gactgtcctt
     3721 tttccttttt cttttttttt cttttccttt ggaaactgac atttgcattt ctccattcca
     3781 agccacggcg taataatatc tgcaatccag cctgaagacc tgcaaatcga aggacctaga
     3841 tcactatcat ctttgtacgt caaggatcct ggttactgta cgtataacct ttctttcttt
     3901 tgctctgacc aatatgaaac actaaaatcg attcgagcag cctctcagca tcaattacag
     3961 tgcgtgaaaa acattcaaat agaggcagca aatattacat gtgaaaatac aggctggcta
     4021 aatccaagct aatttagaaa tgtggtcaaa acgcatactg gcacgtctaa tcgcggattc
     4081 agtaaacaag attaacgatt agcccagtgt ataagtcata tgataccggc atgcgcgaga
     4141 gcatcgctac acccgagctg gcttcatttt cggaggaaaa tcaaaacatt gctttctcct
     4201 gccgtgcgaa ccattcgtca taaaccgtaa tacgcaacat acatttatta ctacatccgt
     4261 taattagcga taattagccg ttattaacaa agagcgctga ggaattcctc tcaaatagcg
     4321 gaggtgcggc ggaggcagag gggcgtaaaa gggcacatgc gtgcctggct caaaaaagga
     4381 tgctccagac tgaaactcag agacaaaaca cgatgcctcg gaggctgaga gcccatcgag
     4441 aggaaaggca aagaaaaggg tcgagtcact cagaggggga ggggagatat tgtgcatcca
     4501 ttggtttgaa ttgaagcagg cagaataaaa atccttgcga ttatgcttcg tgagagatcg
     4561 cgagagagga aaaggctaaa tgccacgtat gcaaatggat aaatgtcatc tatttcttcg
     4621 cgagtagaca ttttgtggac aacgagagtg taaataagcg atatgagggg aatacaaagg
     4681 ccttgaggga aagcgttttg ggcgtgtctt gtcgaaaaga agacagcgat ggcacgcgct
     4741 cagaaccacc atcttcacgt agataatccg cgtgaaaaca ataacaaaac tgctcatttt
     4801 aacacgagca ggtgtttgaa accacccaaa aaagattgtc taaaatatta agattaatta
     4861 atgacgttta aatgtccagg cctgatataa atgaagcgtg tagtaagagt aatcgccttt
     4921 gtttcttttc agagatacgt tttcagagat tcagggaatt atatcttcaa taaaataatg
     4981 tttgatttca ttattttaaa gctttttaag aagtaaaaat acacattatt catttacatt
     5041 ttattttatt taaaggccta tggtcttgaa aataataagt ggaatatata ggctatatat
     5101 tcgtctaagg acagccggtg ttattttata gtgacctcag acaaaagccg agacaaaacg
     5161 aggcgcttgc ctttcatttt aatccttaat agacggtgtg atgaatgaat gaatgaatga
     5221 ataaatgaat gaatgacggg cccttcggca tgttgttccg cagtcctccg tgagcacgag
     5281 tctctaccgc agtgccaagc acataaaacc gttgagtcag ataaccttct ccaggcgcgt
     5341 atacacaatt caaggacaag cacatcacaa ctatacacaa cacaacacaa ccgcctaaac
     5401 gcacgagctc atcaatcgca atttaaaggc atgtcagtca aagaaaaagg cggcgttatt
     5461 taaaaccaat gttcaacaat atttgtatgc tgactagccc gggcgcgcac gctataagat
     5521 cacaaggcct tgttttttcc ctttattcaa aatatcatta attattacag gtcataaaac
     5581 aaaacaatgc gaattacaat gatttaaaga ctattgattt aacaataatc agttaattat
     5641 agaataatat tataatattt tacatacaaa tgaaagatga cattatttga tgacattgta
     5701 attaaaatat atgcaatata tttttacgtt tttattaatt aggctattac tattattata
     5761 attatcatta tttttattat tattattatt attaaatagt cttgcgaaat aagagtgaag
     5821 gcagttgcaa tgttcattgt tcccggagag tcccgcagcc tctggcctca acacaaagcg
     5881 ctactgttta cattaatatt catagtgctg ccatggtctc tgtagaggag gaacgggtgg
     5941 ctcttttgtt cgctagtgtc aatacgttta ttcagcaaaa agcagctcgg atttgtgaat
     6001 gcccaacaag cacacgcgca ctccaatcaa aggacgagag gataacgatt ttaaacgatt
     6061 cgcaaacgtg tttatttgta aagacgacat caagagctta atgtccactt gaaaagaaat
     6121 aaatatgccc gcggcgccaa ggatattttg aatgtagtcg ttcatggtga gccactcccg
     6181 aattcagcac tctggagagc gaccaaagag gaatagcgga atagcaacta cttgagccat
     6241 ttctctccgt ttgatggcgt tcatgattaa aaatataatc acgtgatttt ttatacatat
     6301 ataaatatat acataaagta acgctttcct tttgagaata cataaatatt tattcgcgtg
     6361 ataaatcaat gatcttatat ttatttgcac gaccgaaatt agccaaattc accaattaaa
     6421 aaaaaaataa gccaacaaaa aagaagggtt tatccgttca attttgactt gtgtcctgtt
     6481 gttttacagc tgccactgtg atcccttaag ctgcagtgag agtgtggcta atgccttttg
     6541 tttaagataa tcctgtaatc tgttaccgaa acggcctatt gacaagccgg cattcacatt
     6601 tcagtcagga ggcccgtcca gacatgtaca tttatgaatt acgaatgata aaattaagat
     6661 ctgcattagg cgtcagaaat gtcacgaaca cccattcatt tcccacagcc aataatggat
     6721 catgctggga agcgctatcc tcgcagtctc aaaattaatg ttgacatgtt gcgtagagct
     6781 acagattaga tcagccatat gctttatgtg tttacctcag cggatcttca gcaagatgtg
     6841 tttattttaa gaaaatggct tcctcgctgc ctgcacaggg gcattaagga agtgacgtga
     6901 gcgtcctcac agtcaaatta ctttatctca tgctgctttc aggcctctgc ttattttatt
     6961 attattattt taaattaggg ggaatcacgg tggcgttgtg ggtagggtag cgcgatcacc
     7021 ttacagcaag aaggtcgctg gttcgagctc tggctgggtc agttggcatt tctgtgtgga
     7081 gtttaaattg aattgaaatt gaataaacta aattggccct agtgtatgtg tgtgaatgta
     7141 agtgtgcatg ggtgtttccc agtgttgggt ttgactggaa gagcatccgc ttcgtaaaac
     7201 atatgctgga taagttggcg gttcatttca ctgtggtgac cgctgattaa taaagggact
     7261 aagctgaaaa tgaaatgaat taatacattt taaatgagct tttgctgaca tgtttatgat
     7321 tacaaatagt gaaattgtat gttaatttac gataagtgca ttgttctaaa ttcattttta
     7381 tgtagcagct ggtttttctt ccatttgtgt cattcaacag aacatttttc taaaaaagtg
     7441 aacttaaatt ttctcctccc tctgacaagg cttgatggta atttcagccg actctaatta
     7501 cagactcact aaaaggaatc ggtgcagtca tttatcttta tcagcgcgct ggtgggaagg
     7561 taataggttt tgcttatgag ttgtttgcgt gcgagtctgg gtccttgtgt aggcgatgct
     7621 gatgcactgc attaaatatt gagggagaag tcgctcaaaa atagcaaagt acaggctggg
     7681 gcaggaacgt caaagcccgc tcagtggcca cgtttcttgt ttcgtaagtc ctatttttaa
     7741 tagcgataat tcaggctcag tagaaggaac tccagcacta taagcagtct ctcgcctgtc
     7801 tcggcgagtt atgtattttg ggatgaagtg ctcttccacc tctacctctg ctgtgctggc
     7861 gccagtatct gggctgtaag atagtgtacg tgtcagtctg tgtggccgaa gagcctgtgt
     7921 gttgtgtaac tcactacatt cgtcgctgtg gatggctgac ttgccatggg gaggtttact
     7981 tcagttcacg aatcacattc gccacgatct tgtcactttg tgtcaagact cgctctctgt
     8041 gtgtcaacag ctctgctgtt tgaggttgaa attctgaact tgagatatat cccaggatat
     8101 attagggcta ctgccaacgg aaaaagcaaa tggtgccatg gccatttgat tgatcaaaca
     8161 aacacccttt tgcttgggtg acagtgaaat gtcagatcca tgtttgtact gctttaataa
     8221 cttgcctctc tgagctttct ccttgtcagt gtcaatttgc ctgagatata aattccggtt
     8281 gccccgaaag cttattttat taaaaaaaag ctaacatgca ctcatacaac gtgcgcacat
     8341 acatgtgagg cttgctaaaa gtcaagaagc aagccaatag caagagtcgt cagactgctg
     8401 ttacctgggc aacctggtta gaggtaaggc tcaaggcaag aataaatcat tccactgaag
     8461 agacacagcg ttacagcgag atgagagcaa gaggcgatag tgaagggaga acaaaagaac
     8521 agcctaccca cactagtgca gatctacctg tttgagctac tgattgttca gtttgattag
     8581 atatcagaca attctgctat ctccaattgc attgctgcat gaattcctag aaatcatttt
     8641 aatcaccaaa aactagtagg gacacttgtg gcgtatatga ttctgcctgt tgattgtggg
     8701 gatccaacag aaagatcatg aatgcattgt taattaagtc ggtgagacac cctgttccac
     8761 cctaggggcc gttctgggga aagagtgctt tcagtcagct aaagatgact aatatgtgaa
     8821 catatattac tacacatgcc aatttgtaca ttctggatag ttaccagccc tgggaaaaca
     8881 tcgaaaaaaa taaatcaaca tagataaaat tggcaaatcc cttctggtac gatgcagagt
     8941 gcttgctctg agaacttgtg cattgtgaca gaaagcgacc gttaaaaact gaaagtagca
     9001 tctacaacct gcatacatct gttctttgaa aatcccctgt aggccaaatt aagcatcacg
     9061 ctgtgctcca cagcaacacg gatgaaccga aaacccagac acaaacgcac acacttttca
     9121 cataaacaag gtttagttat gctagctatg ctttttcgtc ctccctttgt catcagcggc
     9181 gagtgacgta acacagtttg accctggaca gcgtaaaggg ggggttggga atgagtgaaa
     9241 ggactgtgac atctgcaggg ccaggggctg atggagagag ctgaagtgag ggaggtcaga
     9301 ggaggaggaa gggtgggata ttgtcctcct caggccctat ccctctcaga cgcattgatt
     9361 tttaacctct cagcgagaat gccaagcatt acacccctca aattatggtt cctcacccaa
     9421 cctgttatat tttccacctg cagcacccag ctttcttgta caaagtggtc actagtcacc
     9481 atgcccccct gccagccaca gcgacccctg ctgctgttgc tgctgctgct ggcctgccag
     9541 ccacaggtcc cctccgctca ggtgatggac ttcctgtttg agaagtggaa gctctacggt
     9601 gaccagtgtc accacaacct gagcctgctg ccccctccca cggagctggt gtgcaacaga
     9661 accttcgaca agtattcctg ctggccggac acccccgcca ataccacggc caacatctcc
     9721 tgcccctggt acctgccttg gcaccacaaa gtgcaacacc gcttcgtgtt caagagatgc
     9781 gggcccgacg gtcagtgggt gcgtggaccc cgggggcagc cttggcgtga tgcctcccag
     9841 tgccagatgg atggcgagga gattgaggtc cagaaggagg tggccaagat gtacagcagc
     9901 ttccaggtga tgtacacagt gggctacagc ctgtccctgg gggccctgct cctcgccttg
     9961 gccatcctgg ggggcctcag caagctgcac tgcacccgca atgccatcca cgcgaatctg
    10021 tttgcgtcct tcgtgctgaa agccagctcc gtgctggtca ttgatgggct gctcaggacc
    10081 cgctacagcc agaaaattgg cgacgacctc agtgtcagca cctggctcag tgatggagcg
    10141 gtggctggct gccgtgtggc cgcggtgttc atgcaatatg gcatcgtggc caactactgc
    10201 tggctgctgg tggagggcct gtacctgcac aacctgctgg gcctggccac cctccccgag
    10261 aggagcttct tcagcctcta cctgggcatc ggctggggtg cccccatgct gttcgtcgtc
    10321 ccctgggcag tggtcaagtg tctgttcgag aacgtccagt gctggaccag caatgacaac
    10381 atgggcttct ggtggatctt gcggttcccc gtcttcctgg ccatcctgat caacttcttc
    10441 atcttcgtcc gcatcgttca gctgctcgtg gccaagctgc gggcacggca gatgcaccac
    10501 acagactaca agttccggct ggccaagtcc acgctgaccc tcatccctct gctgggcgtc
    10561 cacgaagtgg tctttgcctt cgtgacggac gagcacgccc agggcaccct gcgctccgcc
    10621 aagctcttct tcgacctctt cctcagctcc ttccagggcc tgctggtggc tgtcctctac
    10681 tgcttcctca acaaggaggt gcagtcggag ctgcggcggc gttggcaccg ctggcgcctg
    10741 ggcaaagtgc tatgggagga gcggaacacc agcaaccaca gggcctcatc ttcgcccggc
    10801 cacggccctc ccagcaagga gctgcagttt gggaggggtg gtggcagcca ggattcatct
    10861 gcggagaccc ccttggctgg tggcctccct agattggctg agagcccctt cggatccgag
    10921 aacctgtact tccagagccc gcctaaacgc aagacactca atgccgctgc cgaagccaat
    10981 gcccacgctg atggccatgc tgatggcaat gctgatggtc atgtcgctaa cactgcagca
    11041 agcagcaaca acgcccgttt tgcggacttg accaacattg acacacccgg cctcggccct
    11101 accacgacga cgttacttgt cgagcccgct cgttcgaaac gccagagagt ctcgagggcc
    11161 tgtgatcagt gtcgagctgc acgtgaaaag tgtgatggaa tccagccggc ttgcttcccc
    11221 tgtgtgtcgc agggccggtc gtgtacctac caggccagtc ccaagaagcg aggagtccag
    11281 acgggctaca tccgcactct cgaactggct ctggcttgga tgttcgagaa cgttgcccgc
    11341 agcgaggacg ccctccacaa tcttttggtc cgtgatgctg gccagggcag cgctctcctg
    11401 gtcggcaaag actcgcctgc tgcagaacgc ctgcatgcaa gatgggcgac gagtcgagtc
    11461 aacaaaagca tcacccgtct tctctcaggt caggccgcac aagatccatc tgaagacggc
    11521 caatccccgt ccgaagacat aaatgtccaa gatgccgggg caaagacatc cgacttccct
    11581 catgcgcctc acttgacttt ctcggcgcct aagtctagta cagctgagac acgcactcta
    11641 ccaggcccgg tccgaccacc tatttcggca aacaccctgg aaaacaacct tcagccagat
    11701 ggtaccggga tagggaagct accacccaat cattggcgcc tgctggatat ctacttttcc
    11761 tacacgcatt cttggctccc tatcctcgag aagaaagaca tgtaccaagc attgtaccag
    11821 tactctgaac aaggctcgtt gcttccctct gcgaatgtcg agtctggcgt tcatgccgag
    11881 ctctggagcg cgctcgccct ggcgtccttc caggctgctg ctactgctgc atcgagtgct
    11941 acgggtccag cttcagctgc tcatggccat gacaatgcca tcaatccttc acctgcagac
    12001 atatctgaca cagcccgaaa gctcatacct ttggaaagcg ggccgttcca ggttcagcac
    12061 tgcagagcgt tgctgcttct ttgtctcgta agccttgggc gggatgattg ggagtctgct
    12121 tggttgctgg ttggctttgc ggtccgcgtc ctacttgttg ttcgcaccca gttgcctcct
    12181 gatgatgacc ggccacgacc aagaatgcgt gcgctgctcg tcgcgtgctt catcgtggat
    12241 accattgtgt ctatgagaca caacgtgccg gcccatctca agccagacga cattgcggat
    12301 ctgccgttac ctgaagacgg tcaagatcaa tgggagccgt ggacaccatg tgagggctta
    12361 ggcggtgaac acaccatgct gcaaatgttg aggaacccgg catacccttt aagcacattc
    12421 aaccacctat atggcgtgac caagctggtt gctttggagc ttctgccaag aatacgaaca
    12481 tcttcacaga acgctccctt ggagttcagg tcgcggttgc agcaggtaat cggccacaat
    12541 tctcccttca gcgtctttgt cctttcccag gatacagcat cggcttttgt gcctactgca
    12601 taccttaccc gtaccgttta tttatgggca gctgcctttt ctgagcctct caacgaacac
    12661 tactcgcatc ttctgatcga aactcttgat cagtatcaga agcggtttgg tacatatgca
    12721 atcccacctc tgatcccttc tcttctagac tcccttcttg ctttaaagaa acaatcacat
    12781 tcttcagagc ggcatcgaag gcacttggaa gagcttttcc ccgcctactc ctccatttgg
    12841 cctcggggag gccgacacag caatactggc ctccaaccca tacgacaact tgagcttccg
    12901 ccgactgcga ctgccactgc aagtatcatg ccccatgtca tggaacagcc cctgtcaacg
    12961 tcaataaatc cggtcaatga tcggtttaat ggaataccga atcctacccc ctacaatagc
    13021 gatgcggccc tagacgcaat tactcaaacc aatgactacg gatcagtcaa tacccatggc
    13081 attctcagta catatccacc acctgccacc cacttgaacg aggcctcggt ggcccttgcg
    13141 cctggaggtg ctcctcccag accacccccg ccatacgttg atagtacaac gaaccatcct
    13201 ccttaccata gtaacctcgt ccccatggcc aatttcggat attcgactgt agactatgat
    13261 gcgatggtgg atgatctggc atcgatcgag tacacggatg cggttgacgt tgatccgcag
    13321 ttcatgacga acctcgggtt tgtcccaggg tgtaatttta gcgatatcaa cacgtatgag
    13381 caataggacg tcccgcggtg gagctcgaat taattcatcg atgatgatcc agacatgata
    13441 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
    13501 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
    13561 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
    13621 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct agatcagatc
    13681 taatctgatc attcaggcgc gcctcaacca ctccaggcat agctagagcc actgatgcat
    13741 ctatagagct gaagtaaaga tagggttgat gcaaatcgat ttagaatttt caatgtagaa
    13801 aaaacaaaat aaaaagtcta ttcacttgat ggatagatta gtcaaacaaa catagagtag
    13861 gcacttccct tgactctgta aacttttgga tttaacagat tgtgcactcc ttttagttaa
    13921 acacattagt ccctgaaaac taagacccta accataatcc cattgtagac ccacctactc
    13981 gggtgaatat aaagctcgca cagatcatct cacagacatc agaccttggc aaaggagcat
    14041 cctgaacatc tctacacact gaggcaaccc tagggccgcc accatgctgt gttgtatcag
    14101 aagaacaaag cctgtggaga agaatgagga ggctgaccag gaactgcaga gcacggtacc
    14161 gcgggcccgg gatccaccgg ccgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
    14221 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
    14281 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
    14341 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
    14401 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
    14461 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
    14521 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
    14581 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt
    14641 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
    14701 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
    14761 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
    14821 ccccaacgag aagctcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
    14881 tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat
    14941 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
    15001 aaacataaaa tgaatgcaat tgttgttgtt atcgaattct gcaggaattg ggtggcatcc
    15061 ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag
    15121 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat
    15181 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc
    15241 acgagggggg gcccgaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
    15301 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
    15361 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
    15421 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aaggcatgct
    15481 ggggatgcgg tgggctctat ggacgcgtga tctgcgaaga tacggccacg ggtgctcttg
    15541 atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc
    15601 cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc
    15661 attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca
    15721 atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg
    15781 ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc
    15841 caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt
    15901 tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa
    15961 gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt
    16021 tacacctctg gttcttgagg tacctcttcc gcttcctcgc tcactgactc gctgcgctcg
    16081 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    16141 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    16201 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    16261 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    16321 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    16381 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    16441 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    16501 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    16561 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    16621 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    16681 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    16741 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    16801 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    16861 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    16921 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    16981 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    17041 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    17101 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    17161 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    17221 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    17281 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    17341 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    17401 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    17461 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    17521 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    17581 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    17641 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    17701 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    17761 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    17821 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    17881 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    17941 ggggttccgc gcacatttcc ccgaggtacc gatc
//
