LOCUS       sequence-448184-       13636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HIVGKO contains a codon-switched eGFP under the control of the HIV-1
            specific promoter, and mKO2 under the control of the constitutive 
            promoter EF1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Battivelli E, Dahabieh MS, Abdel-Mohsen M, Svensson JP, Tojal Da 
            Silva I, Cohn LB, Gramatica A, Deeks S, Greene WC, Pillai SK, Verdin
            E
  TITLE     Distinct chromatin functional states correlate with HIV latency 
            reactivation in infected primary CD4(+) T cells.
  JOURNAL   Elife. 2018 May 1;7. pii: 34655. doi: 10.7554/eLife.34655.
  PUBMED    29714165
REFERENCE   2  (bases 1 to 13636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-1"; volume: "7. pii: 34655. doi"; pages: " 
            10.7554/eLife.34655"
COMMENT     Sequence Label: HIVGKO
FEATURES             Location/Qualifiers
     source          1..13636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    901..1134
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1319..1363
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1657..1683
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(1930..1932,1933..1935,1936..2646)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     2583..2604
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        2717..2928
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2941..3209
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3247..3256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3253..3903
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     LTR             4103..4736
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4788..4806)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4874..4978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4979..5047,5048..5839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5197..5216)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6010..6598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6499..6518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6679..6695
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6679..6695
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     LTR             6726..7359
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7406..7531
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11542..11659
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acattattgt gccccggctg gttttgcgat tctaaaatgt aataataaga cgttcaatgg
       61 aacaggacca tgtacaaatg tcagcacagt acaatgtaca catggaatta ggccagtagt
      121 atcaactcaa ctgctgttga atggcagtct agcagaagaa gaggtagtaa ttagatctgc
      181 caatttcaca gacaatgcta aaaccataat agtacagctg aaccaatctg tagaaattaa
      241 ttgtacaaga cccaacaaca atacaagaaa aagtatccgt atccagaggg gaccagggag
      301 agcatttgtt acaataggaa aaataggaaa tatgagacaa gcacattgta acattagtag
      361 agcaaaatgg aatgccactt taaaacagat agctagcaaa ttaagagaac aatttggaaa
      421 taataaaaca ataatcttta agcaatcctc aggaggggac ccagaaattg taacgcacag
      481 ttttaattgt ggaggggaat ttttctactg taattcaaca caactgttta atagtacttg
      541 gtttaatagt acttggagta ctgaagggtc aaataacact gaaggaagtg acacaatcac
      601 actcccatgc agaataaaac aatttataaa catgtggcag gaagtaggaa aagcaatgta
      661 tgcccctccc atcagcggac aaattagatg ttcatcaaat attacagggc tgctattaac
      721 aagagatggt ggtaataaca acaatgggtc cgagatcttc agacctggag gaggagatat
      781 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
      841 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
      901 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
      961 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1021 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1081 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1141 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1201 taataaatct ctggaacaga tttggaataa catgacctgg atggagtggg acagagaaat
     1261 taacaattac acaagcttaa tacattcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1321 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1381 aacaaattgg ctgtggtata taaaaatatt cataatgata gtaggaggct tggtaggttt
     1441 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1501 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1561 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ccttggcact
     1621 tatctgggac gatctgcgga gcctgtgcct cttcagctac caccgcttga gagacttact
     1681 cttgattgta acgaggattg tggaacttct gggacgcagg gggtgggaag ccctcaaata
     1741 ttggtggaat ctcctacaat attggagtca ggagctaaag aatagtgctg ttagcttgct
     1801 caatgccaca gccatagcag tagctgaggg gacagatagg gttatagaag tagtacaagg
     1861 agcttgtaga gctattcgcc acatacctag aagaataaga cagggcttgg aaaggatttt
     1921 gctataagca tggtctccaa aggggaagag ctgttcaccg gcgtggtgcc catcctggtc
     1981 gagctggatg gggatgtgaa tgggcataaa tttagcgtgt ccggggaagg ggaaggggat
     2041 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct ccctgtccct
     2101 tggcccaccc tcgtgaccac cctgacttat ggcgtgcagt gcttcagccg ctaccctgac
     2161 catatgaaac agcacgattt cttcaagtcc gccatgcctg aggggtatgt gcaggaacgg
     2221 actatctttt tcaaagacga tgggaattat aagacccggg ccgaagtcaa gttcgagggc
     2281 gacacactcg tgaaccgcat cgaactcaaa gggattgatt ttaaggagga tgggaatatt
     2341 ctggggcaca aactggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2401 cagaagaacg gcattaaagt gaacttcaag atccgccaca acatcgaaga tggcagcgtc
     2461 cagctcgccg atcattatca acagaacacc cccatcggcg acgggcctgt cctcctccct
     2521 gataaccact acctgagcac ccagtccgcc ctgagcaaag accctaatga aaagcgcgat
     2581 cacatggtcc tgctggagtt cgtgacagct gctggcatca ctctggggat ggatgaactc
     2641 tataaataaa gcgaccctcg agtactagga tcgattaggc ggccgcggat ctgcgatcgc
     2701 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     2761 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     2821 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     2881 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga
     2941 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
     3001 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
     3061 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
     3121 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     3181 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagcgcta
     3241 ccggtcgcca ccatggtttc tgtgatcaag cccgaaatga agatgaggta ctacatggac
     3301 ggcagcgtca atggacatga gtttacgatc gaaggggagg gcaccggccg accctacgag
     3361 ggccaccagg agatgaccct gagggtgacg atggcagagg gcggtcccat gcccttcgcc
     3421 ttcgacctgg taagccacgt gttctgctac ggccaccgag tgttcacaaa atacccggag
     3481 gagatcccag actacttcaa gcaggccttt cccgaggggc tgagctggga gaggagcctg
     3541 gaatttgagg acggtggcag tgctagcgtt agcgcccaca tcagtctgag gggcaacacc
     3601 ttttaccaca agagcaagtt cactggcgtt aacttcccag cggacggccc catcatgcag
     3661 aaccagagcg tggactggga gcccagcacc gagaagatca ccgccagcga cggcgtgctg
     3721 aagggcgacg tgaccatgta cctgaagctg gaaggaggtg gcaaccataa gtgccagatg
     3781 aagaccacct acaaggccgc caaggagatt ctggagatgc cgggagacca ctatattgga
     3841 cacaggctgg tccgaaagac tgaaggtaac atcaccgaac aggtggaaga cgccgtggcc
     3901 cactacagct aggctcgaga cctggaaaaa catggagcaa tcacaagtag caatacagca
     3961 gctaccaatg ctgcttgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     4021 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     4081 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     4141 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     4201 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     4261 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     4321 ctgcatggaa tggatgaccc tgagagagaa gtgttagagt ggaggtttga cagccgccta
     4381 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgacatcga
     4441 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     4501 ggggagtggc gagccctcag atgctgcata taagcagctg ctttttgcct gtactgggtc
     4561 tctctggtta gaccagattt gagcctggga gctctctggc taactaggga acccactgct
     4621 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4681 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     4741 cgatgataag ctgtcaaaca tgagaattaa ttcttgaaga cgaaagggcc tcgtgatacg
     4801 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4861 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4921 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4981 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5041 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5101 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5161 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5221 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5281 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5341 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5401 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5461 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5521 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5581 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5641 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5701 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5761 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5821 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5881 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5941 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6001 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6061 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6121 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6661 ctttcctgcg ttatcccctc tagaactagt ggatcttagc cactttttaa aagaaaaggg
     6721 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     6781 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag gggtcagata
     6841 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     6901 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg gaatggatga
     6961 ccctgagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     7021 ccgagagctg catccggagt acttcaagaa ctgctgacat cgagcttgct acaagggact
     7081 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     7141 cagatgctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     7201 tttgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7261 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7321 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     7381 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     7441 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     7501 ctagaaggag agagatgggt gcgagagcgt cggtattaag cgggggagaa ttagataaat
     7561 gggaaaaaat tcggttaagg ccagggggaa agaaacaata taaactaaaa catatagtat
     7621 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg ccttttagag acatcagaag
     7681 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     7741 gatcattata taatacaata gcagtcctct attgtgtgca tcaaaggata gatgtaaaag
     7801 acaccaagga agccttagat aagatagagg aagagcaaaa caaaagtaag aaaaaagcac
     7861 agcaagcagc agctgacaca ggacacagca gccaggtcag ccaaaattac cctatagtgc
     7921 agaacatcca ggggcaaatg gtacatcagg ccatatcacc tagaacttta aatgcatggg
     7981 taaaagtagt agaagagaag gctttcagcc cagaagtgat acccatgttt tcagcattat
     8041 cagaaggagc caccccacaa gatttaaaca ccatgctaaa cacagtgggg ggacatcaag
     8101 cagccatgca aatgttaaaa gagaccatca atgaggaagc tgcagaatgg gatagagtgc
     8161 atccagtgca tgcagggcct attgcaccag gccagatgag agaaccaagg ggaagtgaca
     8221 tagcaggaac tactagtacc cttcaggaac aaataggatg gatgacacat aatccaccta
     8281 tcccagtagg agaaatctat aaaagatgga taatcctggg attaaataaa atagtaagaa
     8341 tgtatagccc taccagcatt ctggacataa gacaaggacc aaaggaaccc tttagagact
     8401 atgtagaccg attctataaa actctaagag ccgagcaagc ttcacaagag gtaaaaaatt
     8461 ggatgacaga aaccttgttg gtccaaaatg cgaacccaga ttgtaagact attttaaaag
     8521 cattgggacc aggagcgaca ctagaagaaa tgatgacagc atgtcaggga gtggggggac
     8581 ccggccataa agcaagagtt ttggctgaag caatgagcca agtaacaaat ccagctacca
     8641 taatgataca gaaaggcaat tttaggaacc aaagaaagac tgttaagtgt ttcaattgtg
     8701 gcaaagaagg gcacatagcc aaaaattgca gggcccctag gaaaaagggc tgttggaaat
     8761 gtggaaagga aggacaccaa atgaaagatt gtactgagag acaggctaat tttttaggga
     8821 agatctggcc ttcctacaag ggaaggccag ggaattttct tcagagcaga ccagagccaa
     8881 cagccccacc atttcttcag agcagaccag agccaacagc cccaccagaa gagagcttca
     8941 ggtctggggt agagacaaca actccctctc agaagcagga gccgatagac aaggaactgt
     9001 atcctttaac ttccctcaga tcactctttg gcaacgaccc ctcgtcacaa taaagatagg
     9061 ggggcaacta aaggaagctc tattagatac aggagcagat gatacagtat tagaagaaat
     9121 gagtttgcca ggaagatgga aaccaaaaat gataggggga attggaggtt ttatcaaagt
     9181 aagacagtat gatcagatac tcatagaaat ctgtggacat aaagctatag gtacagtatt
     9241 agtaggacct acacctgtca acataattgg aagaaatctg ttgactcaga ttggttgcac
     9301 tttaaatttt cccattagtc ctattgaaac tgtaccagta aaattaaagc caggaatgga
     9361 tggcccaaaa gttaaacaat ggccattgac agaagaaaaa ataaaagcat tagtagaaat
     9421 ttgtacagaa atggaaaagg aagggaaaat ttcaaaaatt gggcctgaaa atccatacaa
     9481 tactccagta tttgccataa agaaaaaaga cagtactaaa tggagaaaat tagtagattt
     9541 cagagaactt aataagagaa ctcaagactt ctgggaagtt caattaggaa taccacatcc
     9601 cgcagggtta aaaaagaaaa aatcagtaac agtactggat gtgggtgatg catatttttc
     9661 agttccctta gatgaagact tcaggaagta tactgcattt accataccta gtataaacaa
     9721 tgagacacca gggattagat atcagtacaa tgtgcttcca cagggatgga aaggatcacc
     9781 agcaatattc caaagtagca tgacaaaaat cttagagcct tttagaaaac aaaatccaga
     9841 catagttatc tatcaataca tggatgattt gtatgtagga tctgacttag aaatagggca
     9901 gcatagaaca aaaatagagg agctgagaca acatctgttg aggtggggac ttaccacacc
     9961 agacaaaaaa catcagaaag aacctccatt cctttggatg ggttatgaac tccatcctga
    10021 taaatggaca gtacagccta tagtgctgcc agaaaaagac agctggactg tcaatgacat
    10081 acagaagtta gtgggaaaat tgaattgggc aagtcagatt tacccaggga ttaaagtaag
    10141 gcaattatgt aaactcctta gaggaaccaa agcactaaca gaagtaatac cactaacaga
    10201 agaagcagag ctagaactgg cagaaaacag agagattcta aaagaaccag tacatggagt
    10261 gtattatgac ccatcaaaag acttaatagc agaaatacag aagcaggggc aaggccaatg
    10321 gacatatcaa atttatcaag agccatttaa aaatctgaaa acaggaaaat atgcaagaac
    10381 gaggggtgcc cacactaatg atgtaaaaca attaacagag gcagtgcaaa aaataaccac
    10441 agaaagcata gtaatatggg gaaagactcc taaatttaaa ctacccatac aaaaggaaac
    10501 atgggaaaca tggtggacag agtattggca agccacctgg attcctgagt gggagtttgt
    10561 caatacccct cctttagtga aattatggta ccagttagag aaagaaccca tagtaggagc
    10621 agaaacgttc tatgtagatg gggcagctag cagggagact aaattaggaa aagcaggata
    10681 tgttactaat agaggaagac aaaaagttgt caccctaact gacacaacaa atcagaagac
    10741 tgagttacaa gcaattcatc tagctttgca ggattcggga ttagaagtaa atatagtaac
    10801 agactcacaa tatgcattag gaatcattca agcacaacca gataaaagtg aatcagagtt
    10861 agtcaatcaa ataatagagc agttaataaa aaaggaaaag gtctatctgg catgggtacc
    10921 agcacacaaa ggaattggag gaaatgaaca agtagataaa ttagtcagtg ctggaatcag
    10981 gaaagtacta tttttagatg gaatagataa ggcccaagat gaacatgaga aatatcacag
    11041 taattggaga gcaatggcta gtgattttaa cctgccacct gtagtagcaa aagaaatagt
    11101 agccagctgt gataaatgtc agctaaaagg agaagccatg catggacaag tagactgtag
    11161 tccaggaata tggcaactag attgtacaca tttagaagga aaagttatcc tggtagcagt
    11221 tcatgtagcc agtggatata tagaagcaga agttattcca gcagaaacag ggcaggaaac
    11281 agcatacttt cttttaaaat tagcaggaag atggccagta aaaacaatac atacagacaa
    11341 tggcagcaat ttcaccagta ctacggttaa ggccgcctgt tggtgggcgg gaatcaagca
    11401 ggaatttgga attccctaca atccccaaag tcaaggagta gtagaatcta tgaataaaga
    11461 attaaagaaa attataggcc aggtaagaga tcaggctgaa catcttaaga cagcagtaca
    11521 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
    11581 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
    11641 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggaaagg
    11701 accagcaaag ctcctctgga aaggtgaagg ggcagtagta atacaagata atagtgacat
    11761 aaaagtagtg ccaagaagaa aagcaaagat cattagggat tatggaaaac agatggcagg
    11821 tgatgattgt gtggcaagta gacaggatga ggattagaac atggaaaagt ttagtaaaac
    11881 accatatgta tgtttcaggg aaagctaggg gatggtttta tagacatcac tatgaaagcc
    11941 ctcatccaag aataagttca gaagtacaca tcccactagg ggatgctaga ttggtaataa
    12001 caacatattg gggtctgcat acaggagaaa gagactggca tctgggtcag ggagtctcca
    12061 tagaatggag gaaaaagaga tatagcacac aagtagaccc tgaactagca gaccaactaa
    12121 ttcatctgta ttactttgac tgtttttcag actctgctat aagaaaggcc ttattaggac
    12181 atatagttag ccctaggtgt gaatatcaag caggacataa caaggtagga tctctacaat
    12241 acttggcact agcagcatta ataacaccaa aaaagataaa gccacctttg cctagtgtta
    12301 cgaaactgac agaggataga tggaacaagc cccagaagac caagggccac agagggagcc
    12361 acacaatgaa tggacactag agcttttaga ggagcttaag aatgaagctg ttagacattt
    12421 tcctaggatt tggctccatg gcttagggca acatatctat gaaacttatg gggatacttg
    12481 ggcaggagtg gaagccataa taagaattct gcaacaactg ctgtttatcc atttcagaat
    12541 tgggtgtcga catagcagaa taggcgttac tcaacagagg agagcaagaa atggagccag
    12601 tagatcctag actagagccc tggaagcatc caggaagtca gcctaaaact gcttgtacca
    12661 cttgctattg taaaaagtgt tgctttcatt gccaagtttg tttcacaaca aaagccttag
    12721 gcatctccta tggcaggaag aagcggagac agcgacgaag acctcctcaa ggcagtcaga
    12781 ctcatcaagt ttctctatca aagcagtaag tagtacatgt aatgcaacct atacaaatag
    12841 caatagcagc attagtagta gcaataataa tagcaatagt tgtgtggtcc atagtaatca
    12901 tagaatatag gaaaatatta agacaaagaa aaatagacag gttaattgat agactaatag
    12961 aaagagcaga agacagtggc aatgagagtg aaggagaaat atcagcactt gtggagatgg
    13021 gggtggaaat ggggcaccat gctccttggg atattgatga tctgtagtgc tacagaaaaa
    13081 ttgtgggtca cagtctatta tgggctgtgt ggaaggaagc aaccaccact ctattttgtg
    13141 catcagatgc taaagcatat gatacagagg tacataatgt ttgggccaca catgcctgtg
    13201 tacccacaga ccccaaccca caagaagtag tattggtaaa tgtgacagaa aattttaaca
    13261 tgtggaaaaa tgacatggta gaacagatgc atgaggatat aatcagttta tgggatcaaa
    13321 gcctaaagcc atgtgtaaaa ttaaccccac tctgtgttag tttaaagtgc actgatttgg
    13381 ggaatgctac taataccaat agtagtaata ccaatagtag tagcggggaa atgatgatgg
    13441 agaaaggaga gataaaaaac tgctctttca atatcagcac aagcataaga ggtaaggtgc
    13501 agaaagaata tgcatttttt tataaacttg atataatacc aatagataat gatactacca
    13561 gctatacgtt gacaagttgt aacacctcag tcattacaca ggcctgtcca aaggtatcct
    13621 ttgagccaat tcccat
//
