LOCUS       sequence-448192-       10879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of constitutively active human Akt1 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Xie R, Cheng M, Li M, Xiong X, Daadi M, Sapolsky RM, Zhao H
  TITLE     Akt isoforms differentially protect against stroke-induced neuronal 
            injury by regulating mTOR activities.
  JOURNAL   J Cereb Blood Flow Metab. 2013 Dec;33(12):1875-85. doi: 
            10.1038/jcbfm.2013.132. Epub 2013 Aug 14.
  PUBMED    23942361
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/jcbfm.2013.132"; journalName: "J Cereb Blood Flow Metab"; 
            date: "2013-12"; volume: "33"; issue: "12"; pages: "1875-85"
COMMENT     Sequence Label: pHRIG-Akt1
FEATURES             Location/Qualifiers
     source          1..10879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        171..189
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     171..188
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             214..447
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(join(544..1257,1258..1260,1261..1263))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(547..1257,1258..1260,1261..1263))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(589..610)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     938..957
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1197..1218
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(1264..1272,1273..1275,1276..1850)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1437..1456)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     1666..1683
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(1895..1921)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(1901..1927)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(2990..3031)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      3028..3037
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3156..3180)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3177..3380)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3206..3226)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3381..3684
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3743..3860
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4127..4171)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4356..4589
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5082..5207
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5254..5887
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6055..6189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6079..6098)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6133..6152
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6614..6634)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6764..6829
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(7963..8292)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7977..8112
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8277..8297
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(8367..8384)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8538..9126)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8618..8637)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9297..10088,10089..10157))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9920..9939
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10158..10262)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10330..10348
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10386..10408)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10508..10527
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10741..10760
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttaccagaaa ctcagaaggt tcgtccaacc aaaccgactc tgacggcagt ttacgagaga
       61 gatgataggg tctgcttcag taagccagat gctacacaat taggcttgta catattgtcg
      121 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga
      181 cactatagaa tacacggaat taattctaga tgctgctaga gattttccac actgactaaa
      241 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
      301 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
      361 cccaggctca gatctggtct aaccagagag acccagtaca agcaaaaagc agatcttgtc
      421 ttcgttggga gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat
      481 ctacagctgc cttgtaagtc attggtctta aaggtaccga gctcgaattc cctcgaggcc
      541 gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      601 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
      661 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      721 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
      781 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
      841 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      901 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      961 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
     1021 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac
     1081 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
     1141 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
     1201 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
     1261 catggttgtg gccatattat catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg
     1321 gggggcctag acgttttttt aacctcgact aaacacatgt aaagcatgtg taccgaggcc
     1381 ccagatcaga tcccatacaa tggggtacct tctgggcatc cttcagcccc ttgttgaata
     1441 cgcttgagga gagccatttg actctttcca caactatcca actcacaacg tggcactggg
     1501 gttgtgccgc ctttgcaggt gtatcttata cacgtggctt ttggccgcag aggcacctgt
     1561 cgccaggtgg ggggttccgc tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag
     1621 aagcttccag aggaactgct tccttcacga cattcaacag accttgcatt cctttggcga
     1681 gaggggaaag acccctagga atgctcgtca agaagacagg gccaggtttc cgggccctca
     1741 cattgccaaa agacggcaat atggtggaaa ataacatata gacaaacgca caccggcctt
     1801 attccaagcg gcttcggcca gtaacgttag ggggggggga gggagagggg cggatcccgg
     1861 gcccgcggtg agaaccgttt cacagtccag gtcccagact ggcgtagtcg gggacgtcgt
     1921 aaggataagc catggccgtg ctgctggccg agtaggagaa ctgggggaag tggggcctgc
     1981 gctcgctgtc cacacactcc atgctgtcat cttggtcagg tggtgtgatg gtgatcatct
     2041 gggccgtgaa ctcctcatca aaatacctgg tgtcagtctc cgacgtgacc tggggcttga
     2101 agggtgggct gagcttcttc tcgtacacgt gctgccacac gataccggca aagaagcgat
     2161 gctgcatgat ctccttggcg tcctcggagc ccccgccaag cctctgcttg gggtccttct
     2221 tgagcagccc tgaaagcaag gacttggcct cgggaccaag cgtgcgcggg aagcggatct
     2281 cctccatgag gatgagctca aaaagcttct catggtcctg gttgtagaag ggcaggcgac
     2341 cgcacatcat ctcgtacatg accacgccca gcccccacca gtccactgca cggccgtagt
     2401 cattgtcctc cagcacctcg ggggccaggt actcaggtgt gccgcaaaag gtcttcatgg
     2461 tggcaccgtc cttgatcccc tccttgcaca gcccgaagtc tgtgatctta atgtgcccgt
     2521 ccttgtccag catgaggttc tccagcttga ggtcccggta caccacgttc ttctccgagt
     2581 gcaggtagtc cagggctgac acaatctcag cgccatagaa gcgggcccgg tcctcggaga
     2641 acacacgttc ccgggacagg tggaagaaca gctcgccccc gttggcgtac tccatgacaa
     2701 agcagaggcg gtcgtgggtc tggaaagagt acttcagggc tgtgaggaag gggtgcctgg
     2761 agttctgcag gacgcggttc tcggtgagtg tgtgggccac ctcgtccttg gccacgatga
     2821 cttccttctt gaggatcttc atggcgtagt agcggcctgt ggccttctcc ttcaccagga
     2881 tcaccttgcc gaaagtgccc ttgcccagca gcttcaggta ctcaaactcg ttcatggtca
     2941 cgcggtgctt gggcttggcc agggacacct ccatctcttc agcctcgctg cgctggctgg
     3001 ggtccttagg cttgctcttg ctactcccca tggcggcgaa ttcgatatca agcttatcga
     3061 taccgtcgac tgcagaattc gaagcttgag ctcgagatcc tctagagtcg gtgtcttcta
     3121 tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct
     3181 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
     3241 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
     3301 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
     3361 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
     3421 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
     3481 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
     3541 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
     3601 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
     3661 ccatttaccg taagttatgt aacgcggaac tcccaagctt atcgatccaa agtggatctc
     3721 tgctgtccct gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt
     3781 tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa
     3841 tccccccttt tcttttaaaa gttaaccgat accgtcgaga tccgttcact aatcgaatgg
     3901 atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc
     3961 tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca
     4021 ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga
     4081 ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt
     4141 tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta
     4201 aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt
     4261 gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg
     4321 tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc
     4381 tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca
     4441 gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca
     4501 ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg
     4561 ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca
     4621 ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt
     4681 tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg
     4741 gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct
     4801 tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta
     4861 tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt
     4921 tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc
     4981 ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga
     5041 attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct
     5101 ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg
     5161 cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc
     5221 cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa
     5281 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     5341 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     5401 cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat
     5461 atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag
     5521 tccccagcgg aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga
     5581 tgcagctctc gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact
     5641 tctctctccg ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct
     5701 cctttattgg cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca
     5761 aaggtcagtg gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag
     5821 ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc ttctttggga gtgaattagc
     5881 ccttccaact actaagtttg tagtacatat ttaacaaata caatttcttt aaaatgaaaa
     5941 taattcagag gaatcacagg tttagagtaa atgaaaccac aggtaattgg cagtggtaat
     6001 agggtatggg gtgggaagtt tgggatgatt ttggttagct tgagttatcc agttgatcca
     6061 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     6121 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     6181 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     6241 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag
     6301 tcaaggcact atacatcaaa tattccttat taaccccttt acaaattaaa aagctaaagg
     6361 tacacaattt ttgagcatag ttattaatag cagacactct atgcctgtgt ggagtaagaa
     6421 aaaacagtat gttatgatta taactgttat gcctacttat aaaggttaca gaatattttt
     6481 ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag
     6541 caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt
     6601 ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag
     6661 tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg
     6721 cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa
     6781 ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt
     6841 aaatctctgt aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa
     6901 agatccgggg cccactcata aatccagttg ccgccacggt agccaatcac cgtatcgtat
     6961 aaatcatcgt cggtacgttc ggcatcgctc atcacaatac gtgcctggac gtcgaggatt
     7021 tcgcgtgggt caatgccgcg ccagatccac atcagacggt taatcatgcg ataccagtga
     7081 gggatggttt taccatcaag ggccgactgc acaggcggtt gtgcgccgtg attaaagcgg
     7141 cggactagcg tcgaggtttc aggatgttta aagcggggtt tgaacagggt ttcgctcagg
     7201 tttgcctgtg tcatggatgc agcctccaga atacttactg gaaactattg taacccgcct
     7261 gaagttaaaa agaacaacgc ccggcagtgc caggcgttga aaagattagc gaccggagat
     7321 tggcgggacg aatacgacgc ccatatccca cggctgttca atccaggtat cttgcgggat
     7381 atcaacaaca tagtcatcaa ccagcggacg accagccggt tttgcgaaga tggtgacaaa
     7441 gtgcgctttt ggatacattt cacgaatcgc aaccgcagta ccaccggtat ccaccaggtc
     7501 atcaataacg atgaagcctt cgccatcgcc ttctgcgcgt ttcagcactt taagctcgcg
     7561 ctggttgtcg tgatcgtagc tggaaataca aacggtatcg acatgacgaa tacccagttc
     7621 acgcgccagt aacgcacccg gtacagaccg ccacggctta cggcaataat gcctttccat
     7681 tgttcagaag gcatcagtcg gcttgcgagt ttacgtgcat ggatctgcaa catgtcccag
     7741 gtgacgatgt atttttcgct catgtgaagt gtcccagcct gtttatctac ggcttaaaaa
     7801 gtgttcgagg ggaaaatagg ttgcgcgaga ttatagagat ctggcgcact aaaaaccagt
     7861 atttcacatg agtccgcgtc tttttacgca ctgcctctcc ctgacgcggg ataaagtggt
     7921 attctcaaac atatctcgca agcctgtctt gtgtccaagc tttttgcaaa agcctaggcc
     7981 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     8041 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     8101 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     8161 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     8221 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     8281 taactgacac acattccaca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8341 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8401 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8461 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8521 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8581 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8641 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8701 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8761 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8821 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8881 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8941 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9001 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9061 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9121 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9181 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9241 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9301 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9361 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9421 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9481 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9541 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9601 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9661 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9721 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9781 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9841 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9901 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9961 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10021 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10081 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10141 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10201 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10261 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
    10321 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
    10381 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
    10441 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
    10501 aactatgcgg catcagagca gattgtactg agagtgcacc attcgacgct ctcccttatg
    10561 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
    10621 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
    10681 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
    10741 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
    10801 gatgcgtccg gcgtagagga tctggctagc gatgaccctg ctgattggtt cgctgaccat
    10861 ttccgggtgc gggacggcg
//
