LOCUS       sequence-44821-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pBID-UASC-GRM
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(92..98,99..116,117..122))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(147..271)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    465..559
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    583..677
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
       61 aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
      121 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
      181 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      241 tttctcgttc agcttttttg tacaaacttg ttgatatcga gctctcccgg gaattcgttt
      301 ggtatgcgtc ttgtgattca aagttggctt attcaaagga tattaacgaa ggcagcggca
      361 cgtctgctcg gctcgaacat tcattcacaa ctgatgctct cagccacccc gcgccctttt
      421 ataccgctgc gctcgatccc cgggcgagct cgctagagtc tccgctcgga ggacagtact
      481 ccgctcggag gacagtactc cgctcggagg acagtactcc gctcggagga cagtactccg
      541 ctcggaggac agtactccga cctgcaggca tgcaagcttc cgctcggagg acagtactcc
      601 gctcggagga cagtactccg ctcggaggac agtactccgc tcggaggaca gtactccgct
      661 cggaggacag tactccgacc tgcaggcatg caagcggatc gagccgcggc cagcttggat
      721 ctgttgttgg ttggcacacc acaaatatac tgttgccgag cacaattgat cggctaaatg
      781 gtatggcaag aaaaggtatg caatataata atcttttatt gggtatgcaa cgaaaatttg
      841 tttcgtcaac gtatgcaata ttttttatta aaagagggta tgcaatgtat tttattaaaa
//
