LOCUS       sequence-44823-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pBID-UASC-GV
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(111..235)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(249..251,252..764)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(291..312)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(552..571)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
      121 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      181 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttgata
      241 tcgcatgcgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      301 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      361 cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc
      421 ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
      481 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      541 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
      601 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
      661 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga
      721 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
      781 tcgccgacca ctaccagcag aacaccccca t
//
