LOCUS       sequence-448237-        9688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing human PNPT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Miao R, Jiang C, Chang WY, Zhang H, An J, Ho F, Chen P, Zhang H, 
            Junqueira C, Amgalan D, Liang FG, Zhang J, Evavold CL, 
            Hafner-Bratkovic I, Zhang Z, Fontana P, Xia S, Waldeck-Weiermair M, 
            Pan Y, Michel T, Bar-Peled L, Wu H, Kagan JC, Kitsis RN, Zhang P, 
            Liu X, Lieberman J
  TITLE     Gasdermin D permeabilization of mitochondrial inner and outer 
            membranes accelerates and enhances pyroptosis.
  JOURNAL   Immunity. 2023 Nov 14;56(11):2523-2541.e8. doi: 
            10.1016/j.immuni.2023.10.004. Epub 2023 Nov 3.
  PUBMED    37924812
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2023.10.004"; journalName: "Immunity"; date: 
            "2023-11-14- 14"; volume: "56"; issue: "11"; pages: "2523-2541.e8"
COMMENT     Sequence Label: pLenti-CMV-Puro-PNPT1
FEATURES             Location/Qualifiers
     source          1..9688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    229..250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(265..270,271..288,289..295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     327..343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     362..382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        364..382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        410..636
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             637..817
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    864..989
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1482..1715
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1900..1944
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2242..2262
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2551..2754
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2705..2725
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2792..2801
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2796..2805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5191..5308
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5357..5867
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5786..5804
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5889..6488
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5889..5908)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6385..6405
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6616..6849
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6921..7042
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6958..6977)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7012..7031
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7082..7217
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7237..7256)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7238..7256)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7266..7283)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7266..7282)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7275..7297)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7424..7879
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7511..7530)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7721..7742
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7905..8009
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8010..8078,8079..8870)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8228..8247)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9041..9629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9530..9549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
      361 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
      421 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
      481 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
      541 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
      601 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
      661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      781 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      841 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      901 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      961 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1021 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1081 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1141 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1201 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1261 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1321 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1381 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1441 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1501 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1561 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1621 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1681 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1741 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1801 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1861 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1921 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1981 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2041 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2101 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2161 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct
     2221 cgagcggccg cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata
     2281 cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta
     2341 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
     2401 tgttttaaaa tggactatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2461 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2521 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2581 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2641 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2701 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     2761 gtgaaccgtc agatccgcta gcgctaccgg tgccaccatg gcggcctgca ggtactgctg
     2821 ctcgtgcctc cggctccggc ccctgagcga tggtcctttc cttctgccac ggcgggatcg
     2881 ggcactcacc cagttgcaag tgcgagcact atggagtagc gcagggtctc gagctgtggc
     2941 cgtggactta ggcaacagga aattagaaat atcttctgga aagctggcca gatttgcaga
     3001 tggctctgct gtagtacagt caggtgacac tgcagtaatg gtcacagcgg tcagtaaaac
     3061 aaaaccttcc ccttcccagt ttatgccttt ggtggttgac tacagacaaa aagctgctgc
     3121 agcaggtaga attcccacaa actatctgag aagagagatt ggtacttctg ataaagaaat
     3181 tctaacaagt cgaataatag atcgttcaat tagaccgctc tttccagctg gctacttcta
     3241 tgatacacag gttctgtgta atctgttagc agtagatggt gtaaatgagc ctgatgtcct
     3301 agcaattaat ggcgcttccg tagccctctc attatcagat attccttgga atggacctgt
     3361 tggggcagta cgaataggaa taattgatgg agaatatgtt gttaacccaa caagaaaaga
     3421 aatgtcttct agtactttaa atttagtggt tgctggagca cctaaaagtc agattgtcat
     3481 gttggaagcc tctgcagaga acattttaca gcaggacttt tgccatgcta tcaaagtggg
     3541 agtgaaatat acccaacaaa taattcaggg cattcagcag ttggtaaaag aaactggtgt
     3601 taccaagagg acacctcaga agttatttac cccttcgcca gagattgtga aatatactca
     3661 taaacttgct atggagagac tctatgcagt ttttacagat tacgagcatg acaaagtttc
     3721 cagagatgaa gctgttaaca aaataagatt agatacggag gaacaactaa aagaaaaatt
     3781 tccagaagcc gatccatatg aaataataga atccttcaat gttgttgcaa aggaagtttt
     3841 tagaagtatt gttttgaatg aatacaaaag gtgcgatggt cgggatttga cttcacttag
     3901 gaatgtaagt tgtgaggtag atatgtttaa aacccttcat ggatcagcat tatttcaaag
     3961 aggacaaaca caggtgcttt gtaccgtcac tttcgattca ttagaatctg gtattaagtc
     4021 agatcaagtt ataacagcta taaatgggat aaaagataaa aatttcatgc tgcactacga
     4081 gtttcctcct tatgcaacta atgaaattgg caaagtcact ggtttaaata gaagagaact
     4141 tgggcatggt gctcttgctg agaaagcttt gtatcctgtt attccccgag attttccttt
     4201 caccataaga gttacatctg aagtcctaga gtcaaatggg tcatcttcta tggcatctgc
     4261 atgtggcgga agtttagcat taatggattc aggggttcca atttcatctg ctgttgcagg
     4321 cgtagcaata ggattggtca ccaaaaccga tcctgagaag ggtgaaatag aagattatcg
     4381 tttgctgaca gatattttgg gaattgaaga ttacaatggt gacatggact tcaaaatagc
     4441 tggcactaat aaaggaataa ctgcattaca ggctgatatt aaattacctg gaataccaat
     4501 aaaaattgtg atggaggcta ttcaacaagc ttcagtggca aaaaaggaga tattacagat
     4561 catgaacaaa actatttcaa aacctcgagc atctagaaaa gaaaatggac ctgttgtaga
     4621 aactgttcag gttccattat caaaacgagc aaaatttgtt ggacctggtg gctataactt
     4681 aaaaaaactt caggctgaaa caggtgtaac tattagtcag gtggatgaag aaacgttttc
     4741 tgtatttgca ccaacaccca gtgctatgca tgaggcaaga gacttcatta ctgaaatctg
     4801 caaggatgat caggagcagc aattagaatt tggagcagta tataccgcca caataactga
     4861 aatcagagat actggtgtaa tggtaaaatt atatccaaat atgactgcgg tactgcttca
     4921 taacacacaa cttgatcaac gaaagattaa acatcctact gccctaggat tagaagttgg
     4981 ccaagaaatt caggtgaaat actttggacg tgacccagcc gatggaagaa tgaggctttc
     5041 tcgaaaagtg cttcagtcgc cagctacaac cgtggtcaga actttgaatg acagaagtag
     5101 tattgtaatg ggagaaccta tttcacagtc atcatctaat tctcagtgag aattctcgac
     5161 ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     5221 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     5281 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     5341 ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
     5401 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     5461 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     5521 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     5581 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     5641 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     5701 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     5761 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     5821 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatccaccgg
     5881 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     5941 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     6001 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6061 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6121 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     6181 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     6241 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     6301 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6361 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6421 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6481 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     6541 catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6601 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     6661 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6721 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6781 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6841 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     6901 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     6961 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7021 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7081 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7141 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7201 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     7261 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7321 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7381 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     7441 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7501 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7561 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7621 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7681 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7741 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7801 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7861 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7921 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7981 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8041 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8101 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8161 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8221 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8281 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8341 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8401 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8461 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8521 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8581 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8641 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8701 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8761 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8821 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8881 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8941 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9001 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9061 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9121 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9181 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9241 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9301 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9361 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9421 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9481 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9541 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9601 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9661 tggccttttg ctggcctttt gctcacat
//
