LOCUS       sequence-448238-        9688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing human PNPT1 S484A variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Miao R, Jiang C, Chang WY, Zhang H, An J, Ho F, Chen P, Zhang H, 
            Junqueira C, Amgalan D, Liang FG, Zhang J, Evavold CL, 
            Hafner-Bratkovic I, Zhang Z, Fontana P, Xia S, Waldeck-Weiermair M, 
            Pan Y, Michel T, Bar-Peled L, Wu H, Kagan JC, Kitsis RN, Zhang P, 
            Liu X, Lieberman J
  TITLE     Gasdermin D permeabilization of mitochondrial inner and outer 
            membranes accelerates and enhances pyroptosis.
  JOURNAL   Immunity. 2023 Nov 14;56(11):2523-2541.e8. doi: 
            10.1016/j.immuni.2023.10.004. Epub 2023 Nov 3.
  PUBMED    37924812
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2023.10.004"; journalName: "Immunity"; date: 
            "2023-11-14- 14"; volume: "56"; issue: "11"; pages: "2523-2541.e8"
COMMENT     Sequence Label: pLenti-CMV-Puro-PNPT1 S484A
FEATURES             Location/Qualifiers
     source          1..9688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(60..648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(140..159)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(819..1610,1611..1679))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1442..1461
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1680..1784)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1810..2265)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1947..1968)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2159..2178
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2392..2414
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2406..2423
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2407..2423
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2433..2452
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2433..2451
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2472..2607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2647..2768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2658..2677)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2712..2731
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             2840..3073
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(3201..3800)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3284..3304)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3781..3800
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3822..4332)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3885..3903)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    4381..4498
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      6884..6893
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6888..6897
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6935..7138)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6964..6984)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(7427..7447)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(7745..7789)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7974..8207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8700..8825
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8872..9052
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9053..9279)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(9307..9327)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9307..9325)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9346..9362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9346..9362)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9359..9381)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9370..9386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9394..9400,9401..9418,9419..9424))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9439..9460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9577..9594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
       61 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      121 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      181 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      241 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      301 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      361 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      421 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      481 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      541 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      601 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      661 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      721 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      781 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
      841 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
      901 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
      961 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     1021 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     1081 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     1141 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     1201 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     1261 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     1321 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     1381 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     1441 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     1501 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     1561 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     1621 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     1681 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     1741 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     1801 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     1861 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     1921 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     1981 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     2041 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     2101 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     2161 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     2221 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     2281 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     2341 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     2401 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg
     2461 gtacgtccct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     2521 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     2581 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggatag ctagagccag
     2641 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     2701 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     2761 aacaagttcc tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata
     2821 agatgacatg aactactact gctagagatt ttccacactg actaaaaggg tctgagggat
     2881 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     2941 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     3001 tggtctaacc agagagaccc agtacaagca aaaagcagat cttgtcttcg ttgggagtga
     3061 attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac agctgccttg
     3121 taagtcattg gtcttaaagg taccgatgca tggggtcgtg cgctcctttc ggtcgggcgc
     3181 tgcgggtcgt ggggcgggcg tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt
     3241 ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg
     3301 ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact
     3361 ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg
     3421 gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt
     3481 tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac
     3541 accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc
     3601 gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg
     3661 gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg
     3721 aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc
     3781 gtgggcttgt actcggtcat ggtaagctcc ggtggatccc cctggggaga gaggtcggtg
     3841 attcggtcaa cgagggagcc gactgccgac gtgcgctccg gaggcttgca gaatgcggaa
     3901 caccgcgcgg gcaggaacag ggcccacact accgccccac accccgcctc ccgcaccgcc
     3961 ccttcccggc cgctgctctc ggcgcgccct gctgagcagc cgctattggc cacagcccat
     4021 cgcggtcggc gcgctgccat tgctccctgg cgctgtccgt ctgcgagggt actagtgaga
     4081 cgtgcggctt ccgtttgtca cgtccggcac gccgcgaacc gcaaggaacc ttcccgactt
     4141 aggggcggag caggaagcgt cgccgggggg cccacaaggg tagcggcgaa gatccgggtg
     4201 acgctgcgaa cggacgtgaa gaatgtgcga gacccagggt cggcgccgct gcgtttcccg
     4261 gaaccacgcc cagagcagcc gcgtccctgc gcaaacccag ggctgccttg gaaaaggcgc
     4321 aaccccaacc ccctcgagcc gcggccaaag tggatctctg ctgtccctgt aataaacccg
     4381 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
     4441 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt
     4501 gtggatgaat actgccattt gtctcgaggt cgagaattct cactgagaat tagatgatga
     4561 ctgtgaaata ggttctccca ttacaatact acttctgtca ttcaaagttc tgaccacggt
     4621 tgtagctggc gactgaagca cttttcgaga aagcctcatt cttccatcgg ctgggtcacg
     4681 tccaaagtat ttcacctgaa tttcttggcc aacttctaat cctagggcag taggatgttt
     4741 aatctttcgt tgatcaagtt gtgtgttatg aagcagtacc gcagtcatat ttggatataa
     4801 ttttaccatt acaccagtat ctctgatttc agttattgtg gcggtatata ctgctccaaa
     4861 ttctaattgc tgctcctgat catccttgca gatttcagta atgaagtctc ttgcctcatg
     4921 catagcactg ggtgttggtg caaatacaga aaacgtttct tcatccacct gactaatagt
     4981 tacacctgtt tcagcctgaa gtttttttaa gttatagcca ccaggtccaa caaattttgc
     5041 tcgttttgat aatggaacct gaacagtttc tacaacaggt ccattttctt ttctagatgc
     5101 tcgaggtttt gaaatagttt tgttcatgat ctgtaatatc tccttttttg ccactgaagc
     5161 ttgttgaata gcctccatca caatttttat tggtattcca ggtaatttaa tatcagcctg
     5221 taatgcagtt attcctttat tagtgccagc tattttgaag tccatgtcac cattgtaatc
     5281 ttcaattccc aaaatatctg tcagcaaacg ataatcttct atttcaccct tctcaggatc
     5341 ggttttggtg accaatccta ttgctacgcc tgcaacagca gatgaaattg gaacccctga
     5401 atccattaat gctaaacttc cgccacatgc agatgccata gcagatgacc catttgactc
     5461 taggacttca gatgtaactc ttatggtgaa aggaaaatct cggggaataa caggatacaa
     5521 agctttctca gcaagagcac catgcccaag ttctcttcta tttaaaccag tgactttgcc
     5581 aatttcatta gttgcataag gaggaaactc gtagtgcagc atgaaatttt tatcttttat
     5641 cccatttata gctgttataa cttgatctga cttaatacca gattctaatg aatcgaaagt
     5701 gacggtacaa agcacctgtg tttgtcctct ttgaaataat gctgatccat gaagggtttt
     5761 aaacatatct acctcacaac ttacattcct aagtgaagtc aaatcccgac catcgcacct
     5821 tttgtattca ttcaaaacaa tacttctaaa aacttccttt gcaacaacat tgaaggattc
     5881 tattatttca tatggatcgg cttctggaaa tttttctttt agttgttcct ccgtatctaa
     5941 tcttattttg ttaacagctt catctctgga aactttgtca tgctcgtaat ctgtaaaaac
     6001 tgcatagagt ctctccatag caagtttatg agtatatttc acaatctctg gcgaaggggt
     6061 aaataacttc tgaggtgtcc tcttggtaac accagtttct tttaccaact gctgaatgcc
     6121 ctgaattatt tgttgggtat atttcactcc cactttgata gcatggcaaa agtcctgctg
     6181 taaaatgttc tctgcagagg cttccaacat gacaatctga cttttaggtg ctccagcaac
     6241 cactaaattt aaagtactag aagacatttc ttttcttgtt gggttaacaa catattctcc
     6301 atcaattatt cctattcgta ctgccccaac aggtccattc caaggaatat ctgataatga
     6361 gagggctacg gaagcgccat taattgctag gacatcaggc tcatttacac catctactgc
     6421 taacagatta cacagaacct gtgtatcata gaagtagcca gctggaaaga gcggtctaat
     6481 tgaacgatct attattcgac ttgttagaat ttctttatca gaagtaccaa tctctcttct
     6541 cagatagttt gtgggaattc tacctgctgc agcagctttt tgtctgtagt caaccaccaa
     6601 aggcataaac tgggaagggg aaggttttgt tttactgacc gctgtgacca ttactgcagt
     6661 gtcacctgac tgtactacag cagagccatc tgcaaatctg gccagctttc cagaagatat
     6721 ttctaatttc ctgttgccta agtccacggc cacagctcga gaccctgcgc tactccatag
     6781 tgctcgcact tgcaactggg tgagtgcccg atcccgccgt ggcagaagga aaggaccatc
     6841 gctcaggggc cggagccgga ggcacgagca gcagtacctg caggccgcca tggtggcacc
     6901 ggtagcgcta gcggatctga cggttcacta aaccagctct gcttatatag acctcccacc
     6961 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     7021 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     7081 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     7141 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     7201 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     7261 ttggcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt
     7321 attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc
     7381 caattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata
     7441 ggccctcggt gaagggggcg gccgctcgag gctagtctcg tgatcgatac cgtcgagatc
     7501 cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt
     7561 cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc
     7621 cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc
     7681 ctcctactat cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat
     7741 tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt
     7801 tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc
     7861 actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat
     7921 tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct
     7981 gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc
     8041 agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct
     8101 gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc
     8161 ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc
     8221 ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa
     8281 tttttactac tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc
     8341 caggtctgaa gatcagcggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc
     8401 tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag
     8461 agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt
     8521 tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact
     8581 gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt
     8641 ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg
     8701 acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc
     8761 accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga
     8821 gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga
     8881 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     8941 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     9001 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccgtttatgt
     9061 atcgagctag gcacttaaat acaatatctc tgcaatgcgg caattcagtg gttcgtccaa
     9121 tccatgtcag acccgtctgt tgccttccta ataaggcacg atcgtaccac cttacttcca
     9181 ccaatcggca tgcacggtgc tttttctctc cttgtaaggc atgttgctaa ctcatcgtta
     9241 ccatgttgca agactacaag agtattgcat aagactacat taagcttgca gctccagctt
     9301 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     9361 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     9421 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     9481 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     9541 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9601 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9661 agaatcaggg gataacgcag gaaagaac
//
