LOCUS       sequence-448247-        9986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-fusion protein of ROCK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Chen X, Fansler MM, Janjos U, Ule J, Mayr C
  TITLE     The FXR1 network acts as signaling scaffold for actomyosin 
            remodeling.
  JOURNAL   Cell. 5 August 2024. doi: 10.1016/j.cell.2024.07.015.
  PUBMED    37961296
REFERENCE   2  (bases 1 to 9986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 5 
            August 2024. doi: 10.1016/j.cell.2024.07.015."
COMMENT     Sequence Label: pcDNA3puro-mGFP-ROCK2
FEATURES             Location/Qualifiers
     source          1..9986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     99..119
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    376..497
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(413..432)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     467..486
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(546..562)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(546..562)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(559..581)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    570..586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(594..600,601..618,619..624))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    639..660
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(777..794)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(948..1536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1028..1047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1707..2498,2499..2567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2330..2349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2568..2672)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2747..2766)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2938..3317
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3318..3521
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3472..3492
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3566..3585
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3566..3584
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3608..3610,3611..3613,3614..4324)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3653..3674)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3914..3933)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4261..4282
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8529..8546)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8535..8759
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8805..9233
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8892..8911)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9102..9123
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9242..9262)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9247..9576
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9427..9562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9489..9508
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             join(9589..9986,1..202)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9589..9608)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
       61 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
      121 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
      181 gacccgcaag cccggtgcct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
      241 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
      301 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
      361 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      421 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      481 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
      541 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      601 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      661 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      721 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      781 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      841 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      901 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      961 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1021 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1081 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1141 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1201 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1261 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1321 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1381 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1441 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1501 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1561 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1621 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1681 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1741 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1801 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     1861 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1921 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1981 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2041 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2101 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2161 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2221 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2281 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2341 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2401 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2461 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2521 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2581 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2641 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2701 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     2761 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     2821 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     2881 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     2941 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     3001 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3061 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3121 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3181 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3241 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3301 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3361 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3421 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3481 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     3541 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     3601 agcaaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3661 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3721 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3781 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3841 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3901 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3961 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     4021 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     4081 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     4141 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     4201 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     4261 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     4321 caagatgagc cggcccccgc cgacggggaa aatgcccggc gcccccgaga ccgcgccggg
     4381 ggacggggca ggcgcgagcc gccagaggaa gctggaggcg ctgatccgag accctcgctc
     4441 ccccatcaac gtggagagct tgctggatgg cttaaattcc ttggtccttg atttagattt
     4501 tcctgctttg aggaaaaaca agaacataga taatttctta aatagatatg agaaaattgt
     4561 gaaaaaaatc agaggtctac agatgaaggc agaagactat gatgttgtaa aagttattgg
     4621 aagaggtgct tttggtgaag tgcagttggt tcgtcacaag gcatcgcaga aggtttatgc
     4681 tatgaagctt cttagtaagt ttgaaatgat aaaaagatca gattctgcct ttttttggga
     4741 agaaagagat attatggcct ttgccaatag cccctgggtg gttcagcttt tttatgcctt
     4801 tcaagatgat aggtatctgt acatggtaat ggagtacatg cctggtggag accttgtaaa
     4861 ccttatgagt aattatgatg tgcctgaaaa atgggccaaa ttttacactg ctgaagttgt
     4921 tcttgctctg gatgcaatac actccatggg tttaatacac agagatgtga agcctgacaa
     4981 catgctcttg gataaacatg gacatctaaa attagcagat tttggcacgt gtatgaagat
     5041 ggatgaaaca ggcatggtac attgtgatac agcagttgga acaccggatt atatatcacc
     5101 tgaggttctg aaatcacaag ggggtgatgg tttctatggg cgagaatgtg attggtggtc
     5161 tgtaggtgtt ttcctttatg agatgctagt gggggatact ccattttatg cggattcact
     5221 tgtaggaaca tatagcaaaa ttatggatca taagaattca ctgtgtttcc ctgaagatgc
     5281 agaaatttcc aaacatgcaa agaatctcat ctgtgctttc ttaacagata gggaggtacg
     5341 acttgggaga aatggggtgg aagaaatcag acagcatcct ttctttaaga atgatcagtg
     5401 gcattgggat aacataagag aaacggcagc tcctgtagta cctgaactca gcagtgacat
     5461 agacagcagc aatttcgatg acattgaaga tgacaaagga gatgtagaaa ccttcccaat
     5521 tcctaaagct tttgttggaa atcagctgcc tttcatcgga tttacctact atagagaaaa
     5581 tttattatta agtgactctc catcttgtag agaaactgat tccatacaat caaggaaaaa
     5641 tgaagaaagt caagagattc agaaaaaact gtatacatta gaagaacatc ttagcaatga
     5701 gatgcaagcc aaagaggaac tggaacagaa gtgcaaatct gttaatactc gcctagaaaa
     5761 aacagcaaag gagctagaag aggagattac cttacggaaa agtgtggaat cagcattaag
     5821 acagttagaa agagaaaagg cgcttcttca gcacaaaaat gcagaatatc agaggaaagc
     5881 tgatcatgaa gcagacaaaa aacgaaattt ggaaaatgat gttaacagct taaaagatca
     5941 acttgaagat ttgaaaaaaa gaaatcaaaa ctctcaaata tccactgaga aagtgaatca
     6001 actccagaga caactggatg aaaccaatgc tttactgcga acagagtctg atactgcagc
     6061 ccggttaagg aaaacccagg cagaaagttc aaaacagatt cagcagctgg aatctaacaa
     6121 tagagatcta caagataaaa actgcctgct ggagactgcc aagttaaaac ttgaaaagga
     6181 atttatcaat cttcagtcag ctctagaatc tgaaaggagg gatcgaaccc atggatcaga
     6241 gataattaat gatttacaag gtagaatatg tggcctagaa gaagatttaa agaacggcaa
     6301 aatcttacta gcgaaagtag aactggagaa gagacaactt caggagagat ttactgattt
     6361 ggaaaaggaa aaaagcaaca tggaaataga tatgacatac caactaaaag ttatacagca
     6421 gagcctagaa caagaagaag ctgaacataa ggccacaaag gcacgactag cagataaaaa
     6481 taagatctat gagtccatcg aagaagccaa atcagaagcc atgaaagaaa tggagaagaa
     6541 gctcttggag gaaagaactt taaaacagaa agtggagaac ctattgctag aagctgagaa
     6601 aagatgttct ctattagact gtgacctcaa acagtcacag cagaaaataa atgagctcct
     6661 taaacagaaa gatgtgctaa atgaggatgt tagaaacctg acattaaaaa tagagcaaga
     6721 aactcagaag cgctgcctta cacaaaatga cctgaagatg caaacacaac aggttaacac
     6781 actaaaaatg tcagaaaagc agttaaagca agaaaataac catctcatgg aaatgaaaat
     6841 gaacttggaa aaacaaaatg ctgaacttcg aaaagaacgt caggatgcag atgggcaaat
     6901 gaaagagctc caggatcagc tcgaagcaga acagtatttc tcaacccttt ataaaacaca
     6961 agttagggag cttaaagaag aatgtgaaga aaagaccaaa cttggtaaag aattgcagca
     7021 gaagaaacag gaattacagg atgaacggga ctctttggct gcccaactgg agatcacctt
     7081 gaccaaagca gattctgagc aactggctcg ttcaattgct gaagaacaat attctgattt
     7141 ggaaaaagag aagatcatga aagagctgga gatcaaagag atgatggcta gacacaaaca
     7201 ggaacttacg gaaaaagatg ctacaattgc ttctcttgag gaaactaata ggacactaac
     7261 tagtgatgtt gccaatcttg caaatgagaa agaagaatta aataacaaat tgaaagatgt
     7321 tcaagagcaa ctgtcaagat tgaaagatga agaaataagc gcagcagcta ttaaagcaca
     7381 gtttgagaag cagctattaa cagaaagaac actcaaaact caagctgtga ataagttggc
     7441 tgagatcatg aatcgaaaag aacctgtcaa gcgtggtaat gacacagatg tgcggagaaa
     7501 agagaaggag aatagaaagc tacatatgga gcttaaatct gaacgtgaga aattgaccca
     7561 gcagatgatc aagtatcaga aagaactgaa tgaaatgcag gcacaaatag ctgaagagag
     7621 ccagattcga attgaactgc agatgacatt ggacagtaaa gacagtgaca ttgagcagct
     7681 gcggtcacaa ctccaagcct tgcatattgg tctggatagt tccagtatag gcagtggacc
     7741 aggggatgct gaggcagatg atgggtttcc agaatcaaga ttagaaggat ggctttcatt
     7801 gcctgtacga aacaacacta agaaatttgg atgggttaaa aagtatgtga ttgtaagcag
     7861 taagaagatt cttttctatg acagtgaaca agataaagaa caatccaatc cttacatggt
     7921 tttagatata gacaagttat ttcatgtccg accagttaca cagacagatg tgtatagagc
     7981 agatgctaaa gaaattccaa ggatattcca gattctgtat gccaatgaag gagaaagtaa
     8041 gaaggaacaa gaatttccag tggagccagt tggagaaaaa tctaattata tttgccacaa
     8101 gggacatgag tttattccta ctctttatca tttcccaacc aactgtgagg cttgtatgaa
     8161 gcccctgtgg cacatgttta agcctcctcc tgctttggag tgccgccgtt gccatattaa
     8221 gtgtcataaa gatcatatgg acaaaaagga ggagattata gcaccttgca aagtatatta
     8281 tgatatttca acggcaaaga atctgttatt actagcaaat tctacagaag agcagcagaa
     8341 gtgggttagt cggttggtga aaaagatacc taaaaagccc ccagctccag acccttttgc
     8401 ccgatcatct cctagaactt caatgaagat acagcaaaac cagtctatta gacggccaag
     8461 tcgacagctt gccccaaaca aacctagcta actcgagtct agagggcccg tttaaacccg
     8521 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     8581 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     8641 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     8701 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     8761 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     8821 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     8881 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     8941 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     9001 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     9061 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     9121 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     9181 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     9241 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9301 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     9361 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     9421 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9481 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     9541 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccaaccat gaccgagtac
     9601 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     9661 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     9721 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     9781 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     9841 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     9901 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     9961 ctggccaccg tcggcgtctc gcccga
//
