LOCUS       sequence-448256-        4804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For in vitro transcription of mouse Zc3h12c D252N mutant tagged with
            FLAG-HA at N-term.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Bataclan M, Leoni C, Moro SG, Pecoraro M, Wong EH, Heissmeyer V, 
            Monticelli S
  TITLE     Crosstalk between Regnase-1 and -3 shapes mast cell survival and 
            cytokine expression.
  JOURNAL   Life Sci Alliance. 2024 Jun 3;7(8):e202402784. doi: 
            10.26508/lsa.202402784. Print 2024 Aug.
  PUBMED    38830770
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2024-06-3"; volume: "7(8):e202402784. doi"; 
            pages: " 10.26508/lsa.202402784. Print 2024 Aug"
COMMENT     Sequence Label: pUC57 mini-FLAG-HA-Regnase-3 D252N
FEATURES             Location/Qualifiers
     source          1..4804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(2480..3068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2560..2579)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3239..4030,4031..4099))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3862..3881
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4100..4204)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4244..4263
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4244..4262
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4323..4346
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4359..4385
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4359..4385
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cagtgacatt ttgcaagact ttaaaccaga agagtcccag actccatcta aggaagtgaa
       61 gaaaccccct gatgtggttc gagaatacca aacaaaactg gagtttgcac ttaagttagg
      121 ttattctgaa gaacaggtcc agcttgtgct aaacaaactt ggtactgatg ctttaatcaa
      181 tgatatattg ggagaacttg tcaaacttgg aaataaaagt gaagccgatc aaacagttag
      241 tactattaac agcgtaatgc gagaaacctc atccctggag tctcagaggt ctgaatctcc
      301 aatgcaagag gttgtagtag atgacggtga gaatctgaga ccagtagtta ttaatggcag
      361 caatgtggca atgagccacg gaaacaaaga agtattctcg tgccgaggaa taaaattggc
      421 ggtggattgg tttttggaaa gaggccataa agatatcacg gtctttgttc ctgcttggag
      481 aaaagaacag tcccgccctg acgccctcat cacagatcag gaaattttac gtaaactaga
      541 gaaggaaaaa atcctggtat tcacaccatc acggcgtgtc cagggcaggc gagtggtgtg
      601 ctatgatgac agattcatcg tgaagctggc ttttgagtcc gatggtataa ttgtgtccaa
      661 tgataactac agagatttgg caaatgagaa accagaatgg aagaagttca tagatgagcg
      721 gttattgatg tattcctttg tcaatgacaa gttcatgccc ccagatgacc ctcttggcag
      781 acatggccca agtctggata attttctgag gaagaaaccc attgttcctg agcacaaaaa
      841 gcagccttgt ccctatggaa agaagtgtac gtatggacac aagtgcaaat actaccaccc
      901 agaaaggggc agtcagcctc agcggtcagt ggctgatgaa cttcgtgcca tgtctagaaa
      961 cacagcagcc aaaactacaa atgaaggggg actggtgaaa agcaacagtg ttccttgcag
     1021 caccaaggca gacagcactt cggatgttaa acgcggtgct ccaaagaggc agtcagaccc
     1081 aagcataagg actcacgtct accaagacat agaggagaag cttcccacca aaaacaagtt
     1141 ggaaaccagg tctgtacctt ccttagttag tatcccggcc acttctactg caaaacccca
     1201 aagcactaca cctttaagca atggccttcc atctggagtt catttcccac ctcaggatca
     1261 aagaccacag ggacaatacc ctccaatgat gatggcaacc aaaaatcatg gaacgccaat
     1321 gccttatgaa cagtatccaa aatgtgactc ccctgttgac gttggatatt attccatgtt
     1381 gaatgcatat tctaatctga gtatctcagg cccccgaagt cctgaaaggc gtttctcact
     1441 agacactgat tacagagtaa actctgtggc ttcagactgc agcagtgaag ggagcatgag
     1501 ttgtgggagc agcgactcct acgtggggta caatgaccgc tcctatgtca gctctcctga
     1561 cccacagctg gaagagagtc tcaagtgtca gcacatgcac ccccacagcc gccttaattc
     1621 ccagcccttc ctgcagaatt tccacgatcc cttaaccaga gtccagagtt acagtcacga
     1681 agaaccaaag ttccatccca agcgtcctct tccacacttg gctatgcatc tgcagcaccc
     1741 tgccgtgggt gcccgctcca gctgtcctgg cgattacccc tccccaccaa gttcagcaca
     1801 ttcgaaggca ccacacctag ggaggtcctt agtggccacg agaatagaca gcatttctga
     1861 ttcccgactc tatgatagtt ctccttcaag acaaagaaag ccttattctc gccaggaagg
     1921 cctaggaagc tggggtaggc caagctatgg gctggaagca tatggctatc ggcagacgta
     1981 ctccttaccc gataattcca cgcccccatg ctatgagtcg atcaccttcc agagcctgcc
     2041 tgagcagcag gaacccacct ggagaatccc ctactgtgga atgccacacg atcctccaag
     2101 gtaccaggac aaccgagaga agatttttat caatttgtgt aacatcttcc cccctgacct
     2161 tgtgaggctc gtcatgaaaa ggaatcctca tatgacggat gcccagcagc tcgccgcagc
     2221 catcttagtg gagaaatcgc agctgggtta ctgagcggcc gcctcgaggc tcgctttctt
     2281 gctgtccaat ttctattaaa ggttcctttg ttccctaagt ccaactacta aactggggga
     2341 tattatgaag ggccttgagc atctggattc tgcctaataa aaaacattta ttttcattgc
     2401 aaactagtat cggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2881 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3061 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3121 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3181 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3241 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3301 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3361 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3421 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3481 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3541 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3601 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3661 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3721 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3781 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3841 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3901 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3961 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4021 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4081 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4141 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4201 cgcgcacatt tccccgaaaa gtgccacctg acgtcccaat gattaatacg actcactata
     4261 gggatcgata catttgcttc tgacacaact gtgttcacta gcaacctcaa acagacacca
     4321 tggactacaa ggacgacgat gacaagctcg atggaggata cccctacgac gtgcccgact
     4381 acgccggctc ctccggctgt acaatggctg cggagaagac attgcaggaa tacggggtgc
     4441 tttgcattca agaatacagg aaaagcagca aagtggagtc aagtgcacgg aacagcttca
     4501 tgggcctgaa ggatcacctg gggcatgatc ttggccatct ttatatggag agcactgacc
     4561 cacagatgag tgcagctgtg ccttggccta tggtagagaa accaacaatg gatacagtca
     4621 attcggggaa ggaaggaaaa ggggtgtcag aagagaatgt gagctccggc gactctgagg
     4681 gaagcacgag ttctgatcat gagtcagaac aactgagtag cctttcggta gagccatgct
     4741 cattaaccaa gactcacaga cagctatgtc gctctccctg cttagagcct cgcctactga
     4801 aaca
//
