LOCUS       sequence-44826-e        9115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EXPRESs plasmid for P. falciuparum merozoite surface proteins 
            encoding P. falciparum ama1 with rat CD4d3+4-blac .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, 
            Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner 
            JC, Wright GJ
  TITLE     Basigin is a receptor essential for erythrocyte invasion by 
            Plasmodium falciparum.
  JOURNAL   Nature. 2011 Nov 9;480(7378):534-7. doi: 10.1038/nature10606.
  PUBMED    22080952
REFERENCE   2  (bases 1 to 9115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-11-9- 9"; volume: "480"; issue: "7378"; pages:
            "534-7"
COMMENT     Sequence Label: ama1CD4d3+4-blac
FEATURES             Location/Qualifiers
     source          1..9115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             767..1555
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     polyA_signal    1590..1645
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1644..1663)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3881..3899)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3967..4071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4072..4140,4141..4932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5103..5691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5592..5611
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5845..5862
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5979..6000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6015..6020,6021..6038,6039..6045)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6053..6069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6058..6080
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6077..6093
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6077..6093
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6364..6743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6744..6947
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6898..6918
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6944..6968
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     7449..7468
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      7491..7500
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact
       61 caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc
      121 ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac
      181 tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt
      241 ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta
      301 tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg
      361 tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga
      421 ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg
      481 gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc
      541 caaagggttg aattccggcg gcggctccgg aggcggtacc ggtggcggcg acctagcccc
      601 acagatgctt cgagaactcc aggagactaa tgcggcgctg caagacgtga gagagctctt
      661 gcgacagcag gtcaaggaga tcaccttcct gaagaatacg gtgatggaat gtgacgcctg
      721 cggtctagac agaaacctgc ctccactagc acctctgggc ccacacccag aaacgctggt
      781 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      841 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      901 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      961 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1021 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1081 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1141 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1201 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1261 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1321 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1381 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1441 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1501 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaattaat
     1561 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     1621 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     1681 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     1741 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     1801 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     1861 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     1921 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     1981 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     2041 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     2101 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     2161 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     2221 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     2281 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     2341 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     2401 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     2461 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     2521 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     2581 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     2641 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     2701 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     2761 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     2821 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     2881 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     2941 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     3001 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     3061 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     3121 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     3181 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     3241 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     3301 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     3361 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     3421 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     3481 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     3541 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     3601 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     3661 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     3721 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     3781 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     3841 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     3901 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3961 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4021 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4081 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4141 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4201 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4261 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     4321 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4381 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4441 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4501 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4561 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     4621 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4681 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4741 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4801 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4861 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4921 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4981 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5041 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5101 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5161 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5221 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5281 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5341 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5401 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5461 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5521 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5581 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5641 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5701 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5761 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5821 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     5881 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     5941 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6001 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6061 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     6121 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     6181 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
     6241 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
     6301 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgccat
     6361 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     6421 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     6481 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     6541 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     6601 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
     6661 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
     6721 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
     6781 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     6841 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
     6901 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     6961 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg ggctcgcggt
     7021 tgaggacaaa ctcttcgcgg tctttccagt actcttggat cggaaacccg tcggcctccg
     7081 aacggtactc cgccaccgag ggacctgagc gagtccgcat cgaccggatc ggaaaacctc
     7141 tcgagaaagg cgtctaacca gtcacagtcg caaggtaggc tgagcaccgt ggcgggcggc
     7201 agcgggtggc ggtcggggtt gtttctggcg gaggtgctgc tgatgatgta attaaagtag
     7261 gcggtcttga gacggcggat ggtcgaggtg aggtgtggca ggcttgagat ccagctgttg
     7321 gggtgagtac tccctctcaa aagcgggcat tacttctgcg ctaagattgt cagtttccaa
     7381 aaacgaggag gatttgatat tcacctggcc cgatctggcc atacacttga gtgacaatga
     7441 catccacttt gcctttctct ccacaggtgt ccactcccag gtccaagttt gccaccatgg
     7501 agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtgcg gccgcccaga
     7561 attactggga gcacccttac cagaacagcg acgtgtaccg gcccatcaac gagcacagag
     7621 agcaccccaa agagtacgag taccccctgc accaggaaca cacctaccag caggaagata
     7681 gcggcgagga cgagaacacc ctgcagcacg cctaccccat cgatcacgag ggcgccgaac
     7741 cagcccccca ggaacagaac ctgttcagca gcatcgagat cgtggagcgg agcaactaca
     7801 tgggcaaccc ctggaccgag tacatggcca agtacgacat cgaggaagtg cacggcagcg
     7861 gcatcagagt ggacctgggc gaggatgccg aagtggctgg aacccagtac agactgccca
     7921 gcggcaagtg ccctgtgttc ggcaagggca tcatcatcga gaacagcaac accgcctttc
     7981 tgacccccgt ggccaccggc aaccagtacc tgaaggacgg cggcttcgcc tttcctccca
     8041 ccgagcccct gatgagcccc atgaccctgg acgagatgcg gcacttctac aaggacaata
     8101 agtacgtgaa gaacctggac gagctgaccc tgtgttctcg gcacgccggc aacatgatcc
     8161 ccgacaacga caagaactcc aactacaagt accccgccgt gtacgacgac aaggataaga
     8221 agtgccacat cctgtacatt gccgcccagg aaaacaacgg ccccagatac tgcaacaagg
     8281 acgagagcaa gcggaacagc atgttctgct tcagacccgc caaggacatc agcttccaga
     8341 actacgccta cctgagcaag aacgtggtgg acaactggga gaaagtgtgc ccccggaaga
     8401 atctgcagaa cgccaagttc ggcctgtggg tggacggcaa ctgcgaggac atcccccacg
     8461 tgaacgagtt ccccgccatc gacctgttcg agtgcaacaa gctggtgttc gagctgtccg
     8521 ccagcgacca gcccaagcag tacgagcagc acctgaccga ctacgagaag atcaaagagg
     8581 gcttcaagaa caagaacgcc gccatgatca agagcgcctt tctgcccacc ggcgccttca
     8641 aggccgacag atacaagagc cacggcaagg gctacaactg gggcaactac aacaccgaga
     8701 cacagaagtg cgagatcttc aacgtgaagc ccacctgtct gatcaacaac gccgcctaca
     8761 ttgccaccac cgccctgagc caccccatcg aggtggagaa caacttcccc tgcagcctgt
     8821 acaaggatga gatcatgaaa gagatcgagc gggagagcaa gagaatcaag ctgaacgaca
     8881 acgacgacga gggcaacaag aagatcattg cccccagaat cttcatcagc gacgataagg
     8941 acagcctgaa gtgcccctgc gaccccgaga tggtgtccaa cagcgcctgc cggttcttcg
     9001 tgtgcaaatg cgtggagaga agggccgaag tgaccagcaa caacgaggtg gtggtcaaag
     9061 aggaatacaa ggacgaatac gccgacatcc ccgagcacaa gcccaccggc gcgcc
//
