LOCUS       sequence-448260-        9926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express mouse Regnase-3 D252N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Bataclan M, Leoni C, Moro SG, Pecoraro M, Wong EH, Heissmeyer V, 
            Monticelli S
  TITLE     Crosstalk between Regnase-1 and -3 shapes mast cell survival and 
            cytokine expression.
  JOURNAL   Life Sci Alliance. 2024 Jun 3;7(8):e202402784. doi: 
            10.26508/lsa.202402784. Print 2024 Aug.
  PUBMED    38830770
REFERENCE   2  (bases 1 to 9926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2024-06-3"; volume: "7(8):e202402784. doi"; 
            pages: " 10.26508/lsa.202402784. Print 2024 Aug"
COMMENT     Sequence Label: pCDH-Regnase-3 D252N-T2A-copGFP
FEATURES             Location/Qualifiers
     source          1..9926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(217..261)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    446..679
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1172..1297
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1344..1524
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1525..1751)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1766..1783)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1766..1782)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1775..1797)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1991..2010)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2110..2132
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2170..2188)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2256..2360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2361..2429,2430..3221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2579..2598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3392..3980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4134..4151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4268..4289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4304..4309,4310..4327,4328..4334)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4342..4358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4347..4369
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4366..4382
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4366..4382
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      4415..4550
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4590..4711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4601..4620)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4655..4674
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4783..5016
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5090..5678
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5196..5207
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5605..5625
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5769..6431)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             complement(6462..6515)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      9176..9185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             9225..9493
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(9506..9717)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    9760..9876
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg
       61 gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
      121 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
      181 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
      241 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
      301 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
      361 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
      421 ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc
      481 caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg
      541 cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct
      601 ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa
      661 cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt
      721 tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt tatataattc
      781 acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagtg gccgcttgct
      841 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
      901 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
      961 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
     1021 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
     1081 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
     1141 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
     1201 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
     1261 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
     1321 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
     1381 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     1441 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     1501 gatctggtct aaccagagag acccgtttat gtatcgagct aggcacttaa atacaatatc
     1561 tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc agacccgtct gttgccttcc
     1621 taataaggca cgatcgtacc accttacttc caccaatcgg catgcacggt gctttttctc
     1681 tccttgtaag gcatgttgct aactcatcgt taccatgttg caagactaca agagtattgc
     1741 ataagactac acgcgtcagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1801 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1861 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1921 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     1981 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     2041 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     2101 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     2161 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     2221 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2281 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2341 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2401 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     2461 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     2521 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2581 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2641 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2701 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2761 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2821 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2881 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2941 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3001 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3061 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3121 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3181 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     3241 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3301 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3361 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3421 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3481 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3541 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3601 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3661 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3721 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3781 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4141 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4201 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     4261 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     4321 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     4381 acatgattac gaatttggtc tctgcaaaag tctaggcctc caaaaaagcc tcctcactac
     4441 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     4501 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     4561 aggggcggga tagctagagc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4621 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4681 tgtaaccatt ataagctgca ataaacaagt tcctctcact ctctgatatt catttctttg
     4741 caagttataa atactgaata ataagatgac atgaactact actgctagag attttccaca
     4801 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
     4861 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
     4921 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa gcaaaaagca
     4981 gatcttatct tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt
     5041 ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtaccagg cggggaggcg
     5101 gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga
     5161 gccggcagca ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag
     5221 aaggacgtcc cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat
     5281 gatttccccg acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag
     5341 cgggcaaggc aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc
     5401 ccggaaagga gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac
     5461 acagtgcaca ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc
     5521 aaccaggatt tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac
     5581 aaaggcatta aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca
     5641 gtcaatcttt cacaaatttt gtaatccaga ggttgattgt cgacttagat tcagctctag
     5701 cgagatccgg tggagccggg tccggcggtg ccgtccacgg cagaattgga cgactgagcg
     5761 cgggatctgg cgaaggcgat gggggtcttg aaggcgtgct ggtactccac gatgcccagc
     5821 tcggtgttgc tgtgcagctc ctccacgcgg cggaaggcga acatggggcc cccgttctgc
     5881 aggatgctgg ggtggatggc gctcttgaag tgcatgtggc tgtccaccac gaagctgtag
     5941 tagccgccgt cgcgcaggct gaaggtgcgg gcgaagctgc ccaccagcac gttatcgccc
     6001 atggggtgca ggtgctccac ggtggcgttg ctgcggatga tcttgtcggt gaagatcacg
     6061 ctgtcctcgg ggaagccggt gcccaccacc ttgaagtcgc cgatcacgcg gccggcctcg
     6121 tagcggtagc tgaagctcac gtgcagcacg ccgccgtcct cgtacttctc gatgcgggtg
     6181 ttggtgtagc cgccgttgtt gatggcgtgc aggaaggggt tctcgtagcc gctggggtag
     6241 gtgccgaagt ggtagaagcc gtagcccatc acgtggctca gcaggtaggg gctgaaggtc
     6301 agggcgcctt tggtgctctt catcttgttg gtcatgcggc cctgcttggg ggtgccctct
     6361 ccgccgccca ccagctcgaa ctccacgccg ttcagggtgc cggtgatgcg gcactcgatc
     6421 tccatggcgg gcaggccgct ctcgtcgctc tccattccgg aagggccggg attctcctcc
     6481 acgtcaccgc atgttagaag acttcctctg ccctcagcgg ccgctccgta acccagctgc
     6541 gatttctcca ctaagatggc tgcggcgagc tgctgggcat ccgtcatatg aggattcctt
     6601 ttcatgacga gcctcacaag gtcagggggg aagatgttac acaaattgat aaaaatcttc
     6661 tctcggttgt cctggtacct tggaggatcg tgtggcattc cacagtaggg gattctccag
     6721 gtgggttcct gctgctcagg caggctctgg aaggtgatcg actcatagca tgggggcgtg
     6781 gaattatcgg gtaaggagta cgtctgccga tagccatatg cttccagccc atagcttggc
     6841 ctaccccagc ttcctaggcc ttcctggcga gaataaggct ttctttgtct tgaaggagaa
     6901 ctatcataga gtcgggaatc agaaatgctg tctattctcg tggccactaa ggacctccct
     6961 aggtgtggtg ccttcgaatg tgctgaactt ggtggggagg ggtaatcgcc aggacagctg
     7021 gagcgggcac ccacggcagg gtgctgcaga tgcatagcca agtgtggaag aggacgcttg
     7081 ggatggaact ttggttcttc gtgactgtaa ctctggactc tggttaaggg atcgtggaaa
     7141 ttctgcagga agggctggga attaaggcgg ctgtgggggt gcatgtgctg acacttgaga
     7201 ctctcttcca gctgtgggtc aggagagctg acataggagc ggtcattgta ccccacgtag
     7261 gagtcgctgc tcccacaact catgctccct tcactgctgc agtctgaagc cacagagttt
     7321 actctgtaat cagtgtctag tgagaaacgc ctttcaggac ttcgggggcc tgagatactc
     7381 agattagaat atgcattcaa catggaataa tatccaacgt caacagggga gtcacatttt
     7441 ggatactgtt cataaggcat tggcgttcca tgatttttgg ttgccatcat cattggaggg
     7501 tattgtccct gtggtctttg atcctgaggt gggaaatgaa ctccagatgg aaggccattg
     7561 cttaaaggtg tagtgctttg gggttttgca gtagaagtgg ccgggatact aactaaggaa
     7621 ggtacagacc tggtttccaa cttgtttttg gtgggaagct tctcctctat gtcttggtag
     7681 acgtgagtcc ttatgcttgg gtctgactgc ctctttggag caccgcgttt aacatccgaa
     7741 gtgctgtctg ccttggtgct gcaaggaaca ctgttgcttt tcaccagtcc cccttcattt
     7801 gtagttttgg ctgctgtgtt tctagacatg gcacgaagtt catcagccac tgaccgctga
     7861 ggctgactgc ccctttctgg gtggtagtat ttgcacttgt gtccatacgt acacttcttt
     7921 ccatagggac aaggctgctt tttgtgctca ggaacaatgg gtttcttcct cagaaaatta
     7981 tccagacttg ggccatgtct gccaagaggg tcatctgggg gcatgaactt gtcattgaca
     8041 aaggaataca tcaataaccg ctcatctatg aacttcttcc attctggttt ctcatttgcc
     8101 aaatctctgt agttatcatt ggacacaatt ataccatcgg actcaaaagc cagcttcacg
     8161 atgaatctgt catcatagca caccactcgc ctgccctgga cacgccgtga tggtgtgaat
     8221 accaggattt tttccttctc tagtttacgt aaaatttcct gatctgtgat gagggcgtca
     8281 gggcgggact gttcttttct ccaagcagga acaaagaccg tgatatcttt atggcctctt
     8341 tccaaaaacc aatccaccgc caattttatt cctcggcacg agaatacttc tttgtttcca
     8401 tggctcattg ccacattgct gccattaata actactggtc tcagattctc accgtcatct
     8461 actacaacct cttgcattgg agattcagac ctctgagact ccagggatga ggtttctcgc
     8521 attacgctgt taatagtact aactgtttga tcggcttcac ttttatttcc aagtttgaca
     8581 agttctccca atatatcatt gattaaagca tcagtaccaa gtttgtttag cacaagctgg
     8641 acctgttctt cagaataacc taacttaagt gcaaactcca gttttgtttg gtattctcga
     8701 accacatcag ggggtttctt cacttcctta gatggagtct gggactcttc tggtttaaag
     8761 tcttgcaaaa tgtcactgtg tttcagtagg cgaggctcta agcagggaga gcgacatagc
     8821 tgtctgtgag tcttggttaa tgagcatggc tctaccgaaa ggctactcag ttgttctgac
     8881 tcatgatcag aactcgtgct tccctcagag tcgccggagc tcacattctc ttctgacacc
     8941 ccttttcctt ccttccccga attgactgta tccattgttg gtttctctac cataggccaa
     9001 ggcacagctg cactcatctg tgggtcagtg ctctccatat aaagatggcc aagatcatgc
     9061 cccaggtgat ccttcaggcc catgaagctg ttccgtgcac ttgactccac tttgctgctt
     9121 ttcctgtatt cttgaatgca aagcaccccg tattcctgca atgtcttctc cgcagccatg
     9181 gtggcgaatt cgctagctct agagtaggcg ccggtcacag cttggatctg taacggcgca
     9241 gaacagaaaa cgaaacaaag acgtagagtt gagcaagcag ggtcaggcaa agcgtggaga
     9301 gccggctgag tctaggtagg ctccaaggga gcgccggaca aaggcccggt ctcgacctga
     9361 gctttaaact tacctagacg gcggacgcag ttcaggaggc accacaggcg ggaggcggca
     9421 gaacgcgact caaccggcgt ggatggcggc ctcaggtagg gcggcgggcg cgtgaaggag
     9481 agatgcgagc ccctcgaagc ttcagctgtg ttctggcggc aaacccgttg cgaaaaagaa
     9541 cgttcacggc gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga
     9601 gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag gcacccgttc
     9661 aattgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc tctgcccact
     9721 gacgggcacc ggagcgatcg cagatccact agtatcgata aaattttgaa ttttgtaatt
     9781 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
     9841 gcactgtacc ccccaatccc cccttttctt ttaaaagtta accgataccg tcgagatccg
     9901 ttcactaatc gaatggatct gtctct
//
