LOCUS       sequence-448273-        5787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BPLF1_1-1000
FEATURES             Location/Qualifiers
     source          1..5787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3666..3765)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3782..3801)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3783..3801)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3806..3822)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3806..3822)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4253..5044
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5137..5725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5626..5645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg agtaacggcg actgggggca aagccagcgc acccggggaa ccggccccgt
      721 gcgcggaatc aggaccatgg atgtgaatgc ccccgggggc gggagtggag gctcggccct
      781 ccgcatccta ggcacggcct cgtgcaacca ggcccactgc aagtttggcc gctttgccgg
      841 catccagtgc gtcagcaact gcgtcctcta cctggtcaag agcttcctgg ccggccgccc
      901 cctgacctcc cgccctgagc tggacgaggt cctggacgag ggggcgcggc tggatgccct
      961 catgcgccag agcggcatcc tcaaggggca cgagatggcc cagttgacgg acgtgcccag
     1021 ctccgtggtc ctgaggggcg gtgggcgcgt gcacatatac cgctcggcgg agatctttgg
     1081 cctcgtccta ttccctgccc agatcgcaaa ctcggcagtt gttcagtccc tggccgaggt
     1141 cctgcacggc agttacaacg gggtggccca gttcatcctc tacatctgcg acatctacgc
     1201 gggggccatc atcatcgaga cagatggctc cttctacttg tttgaccccc actgccagaa
     1261 ggatgcggcc ccgggcaccc ccgcccatgt cagagtcagc acctatgcgc acgacatcct
     1321 gcagtacgtg ggggcaccag gggcccagta cacttgcgtg catctctatt ttctacccga
     1381 ggcctttgag acagaggacc cccgcatctt tatgctggaa cactatggcg tgtatgactt
     1441 ttatgaggcc aacggcagcg gctttgacct ggtggggccc gagcttgtgt ccagtgatgg
     1501 ggaggcggcg gggacgcccg gtgctgatag cagccctccc gtcatgctac cgtttgaacg
     1561 gcgaataata ccgtataacc tcaggccact gccctctcga tctttcacat ccgactcatt
     1621 ccctgccgcc aggtatagtc ctgcaaagac caactccccg ccgtcgtctc cggcctctgc
     1681 ggccccggcc tctgcggccc cggcctctgc ggccccggcc tccgcggccc cggcctccgc
     1741 ggccccggcc tccgcggccc cggcctccgc ggccccggcc tccgcggccc cggcctccag
     1801 ccctcccctc ttcatcccca tccccggcct aggtcacacc cccggggtcc ccgctccctc
     1861 cacgccacca agggcgtcga gcggcgcagc gcctcagact cccaagagga aaaagggatt
     1921 gggcaaagat agcccccaca agaagcccac ctccggccgc cgcctccctc tgtcctccac
     1981 cactgacaca gaggatgatc agctgccacg cacccacgtt ccgccccatc gccccccttc
     2041 agcagctcgc cttccccctc cagtaatccc catccctcac caatcaccac ccgcctcccc
     2101 gacaccccac cccgcgccag tctcaactat cgctccttca gtcactccat ctcctcgcct
     2161 ccctttgcaa atacccatcc ctctgccaca ggcagctccc tcgaacccaa aaatccctct
     2221 gaccacccca tccccttccc cgactgcagc agcagccccc accaccacga ctctttctcc
     2281 tcctccaact cagcagcagc ccccgcaatc agccgccccg gctccttctc cacttcttcc
     2341 tcagcagcag cccacgccat cagccgcccc ggctccttct ccacttcttc ctcagcagca
     2401 gcccccgcca tcagccgccc gggctccttc tcctcttcct cctcagcagc agcccctgcc
     2461 atcagctacc ccggctcctc ctcctgctca gcagctccca ccatcagcca cgacactcga
     2521 accagaaaaa aaccatcccc cggccgctga cagggctgga acggagatat ccccatcacc
     2581 tcccttcggg cagcagccct cctttgggga tgatgcatct gggggttctg gcctcgtccg
     2641 ctacctgtct gacctagagg agcctttcct atccatgtcc gactctgaag aagcggagag
     2701 cgacttagcc tccgatatcc ccaccacaga ggatgaagac atgttcgaag atgaagtgtt
     2761 ctccaacagc ctggaatcgg gatccagtgc ccccacgtcc cccatcacac tggacaccgc
     2821 caggagccaa tactatcaga ccacctttga catagagact cctgagatgg actttgtgcc
     2881 cctcgagagc aacattgcga gaatagccgg acatacctac caggaacagg ccatcgtcta
     2941 cgacccggcc tctaaccggg aagtgccaga ggcagatgcc ttgagcatga tagactatct
     3001 tcttgtgaca gtggtcctcg agcaggggct aataaggagt agggaccgat cgtctgtgct
     3061 aaatctgctg gaattcttaa aggactggtc gggacatctg caggtcccca cgctggacct
     3121 ggagcaactg ctcacttccg aacttaacat ccaaaacctc gcaaacatgc tatcagaaaa
     3181 caagggaaga gcgggagagt tccataagca cctggccgca aagctggagg cctgcctgcc
     3241 gtccctggcc acgaaggacg cggtccgtgt agatgccggt gctaagatgc tggctgaaat
     3301 tccccagctg gccgagagcg acgatggaaa atttgacctg gaggcggccc gcagacgcct
     3361 gaccgacctg ctctcgggcg gagatcagga ggcgggggaa ggaggaggag aacccgagga
     3421 taattccatc tacaggggcc cgcacgtgga cgtccccttg gtcctggacg acgaaagctg
     3481 gaagcgcctc ctatccctgg cggaggccgc ccgaacggcc gtggccagac aacaggctgg
     3541 ggtagatgag gaggacgtcc gctttttggc gctgttgaca gcgatagagt atggggcccc
     3601 tcccgccgcc tcagtgcccc cctttgtcca caacgtggcc gtccggtcca agaatgcggc
     3661 cctgcaccca actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     3721 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     3781 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     3841 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac
     3901 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc
     3961 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     4021 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     4081 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     4141 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     4201 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga
     4261 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     4321 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     4381 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     4441 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     4501 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     4561 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     4621 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     4681 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     4741 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     4801 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     4861 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     4921 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     4981 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     5041 ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     5101 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5161 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5221 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5281 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5341 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5401 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5461 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5521 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5581 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5641 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5701 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5761 cttttgctgg ccttttgctc acatgtt
//
