LOCUS       sequence-448295-        9171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing 
            stable cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Huang M, Yao F, Nie L, Wang C, Su D, Zhang H, Li S, Tang M, Feng X, 
            Yu B, Chen Z, Wang S, Yin L, Mou L, Hart T, Chen J
  TITLE     FACS-based genome-wide CRISPR screens define key regulators of DNA 
            damage signaling pathways.
  JOURNAL   Mol Cell. 2023 Aug 3;83(15):2810-2828.e6. doi: 
            10.1016/j.molcel.2023.07.004.
  PUBMED    37541219
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.07"; journalName: "Mol Cell"; date: 
            "2023-08-3- 3"; volume: "83"; issue: "15"; pages: "2810-2828.e6"
COMMENT     Sequence Label: pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    229..250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(265..270,271..288,289..295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     327..343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     362..382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        364..382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        410..636
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             637..817
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    864..989
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1482..1715
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1900..1944
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2211..2327
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2350..2653
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2654..2857
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2808..2828
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2854..2878
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2933..2977
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             3011..3034
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3038..3061
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3071..3184
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    3290..3314
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4185..4209)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4240..4828
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4293..4313)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4711..4722)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4842..5341
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4877..4900)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5274..5293
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5362..5961
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5362..5381)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5858..5878
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6099..6332
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6404..6525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6441..6460)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6495..6514
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6565..6700
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6720..6739)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6721..6739)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6749..6766)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6749..6765)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6758..6780)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6907..7362
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6994..7013)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7204..7225
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7388..7492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7493..7561,7562..8353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7711..7730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8524..9112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9013..9032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
      361 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
      421 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
      481 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
      541 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
      601 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
      661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      781 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      841 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      901 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      961 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1021 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1081 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1141 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1201 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1261 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1321 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1381 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1441 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1501 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1561 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1621 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1681 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1741 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1801 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1861 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1921 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1981 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2041 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2101 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2161 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga
     2221 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2281 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2341 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2401 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2461 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2521 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2581 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2641 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2701 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2761 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2821 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2881 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactca tgaaagaaac
     2941 cgctgctgct aaattcgaac gccagcacat ggacagcgga gccggcgcag gagccggcgc
     3001 tgacgcgcct gactacaaag acgatgacga caagggagat tacaaggatg acgatgacaa
     3061 aggcgcgtcg atggacgaga agaccaccgg ctggcggggc ggccacgtgg tggagggcct
     3121 ggccggcgag ctggagcagc tgcgggccag gctggagcac caccctcagg gccagcggga
     3181 gccctccggc ggctgcaagc tgggcggcgc gcctgaattc tgcagtcgac ggtaccgcgg
     3241 gcccatcgct cgagagatag tccagtgtgg tggaattctg cagatatcaa caagtttgta
     3301 caaaaaagca ggctccacca tgacggcccc ctgcccgccg ccacctccag acccccagtt
     3361 tgtcctccga ggcacccagt caccggtgca tgcgctgcac ttctgcgaag gagcccaggc
     3421 tcaggggcgc ccgctcctct tctcagggtc tcagagtggc ctggtacaca tctggagcct
     3481 gcagacgcgg agagcggtta ccaccctgga tggccacggc ggccagtgtg tgacctggct
     3541 gcagacgctg ccccaggggc gccagctcct cagtcagggc cgggacctga agctgtgcct
     3601 gtgggacctc gcggagggca ggagcgctgt cgtggactcc gtgtgcttgg agagtgtggg
     3661 cttctgccgg agcagcatcc tggccggggg ccagccacgc tggacgcttg ccgtgccagg
     3721 gaggggcagc gacgaggttc agattctgga gatgccctcc aagacgtcag tgtgcgccct
     3781 gaagccgaag gcagatgcca agctgggcat gcccatgtgc ctgcggctgt ggcaggccga
     3841 ctgcagctcc cgcccactcc ttctggccgg ctatgaggat ggatcggtgg tcctgtggga
     3901 cgtctctgag cagaaggtgt gcagccgcat cgcctgccat gaggagcccg tcatggacct
     3961 tgactttgac tcccagaagg ccaggggcat ctcaggctcc gcggggaagg cgctggctgt
     4021 ctggagcctg gactggcagc agcggacgat gcagccactg gccgtgctgg ccttccacag
     4081 cgccgctgtc cagtgcgtgg ccttcaccgc cgatggcttg ctggccgcgg gctccaagga
     4141 tcagcggatc agcctctggt cactctaccc acgcgcatgg atccacccag ctttcttgta
     4201 caaagtggtt gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa
     4261 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4321 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4381 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4441 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4501 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4561 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4621 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     4681 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4741 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4801 cggatctccc tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt
     4861 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     4921 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     4981 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     5041 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     5101 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     5161 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     5221 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     5281 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     5341 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5401 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5461 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5521 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5581 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5641 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5701 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     5761 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     5821 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     5881 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     5941 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     6001 gcacgacccc atgcatctcg agggcccggt acctttaaga ccaatgactt acaaggcagc
     6061 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca
     6121 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     6181 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6241 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6301 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     6361 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     6421 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6481 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     6541 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6601 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     6661 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     6721 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     6781 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6841 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     6901 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6961 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7021 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7081 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7141 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7201 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7261 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7321 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     7381 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7441 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7501 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7561 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7621 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7681 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7741 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7801 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7861 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7921 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7981 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8041 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8101 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8161 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8221 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8281 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8341 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8401 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8461 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8521 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8581 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8641 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8701 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8761 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8821 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8881 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8941 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9001 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9061 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9121 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca t
//
