LOCUS       sequence-448301-       11539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for ActivinA and BFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Yamada T, Trentesaux C, Brunger JM, Xiao Y, Stevens AJ, Martyn I, 
            Kasparek P, Shroff NP, Aguilar A, Bruneau BG, Boffelli D, Klein OD, 
            Lim WA
  TITLE     Synthetic organizer cells guide development via spatial and 
            biochemical instructions.
  JOURNAL   Cell. 2024 Dec 11:S0092-8674(24)01323-0. doi: 
            10.1016/j.cell.2024.11.017.
  PUBMED    39706189
REFERENCE   2  (bases 1 to 11539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Dec 11:S0092-8674(24)01323-0. doi: 10.1016/j.cell.2024.11.017."
COMMENT     Sequence Label: PKMC356_pTRE-ActivinA_IRES_BFP_WPRE
FEATURES             Location/Qualifiers
     source          1..11539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..36)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(21..37)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             127..360
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(385..403)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(386..403)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(693..712)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(926..945)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1045..1067
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1105..1123)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(1302..1890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1382..1401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1993..2097
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2098..2166,2167..2958)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2316..2335)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     3200..3217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3287..3307)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3292..3621
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3472..3607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4755..4820
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4950..4970
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5395..5529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5432..5451)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5486..5505
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5697..6330
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6377..6502
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6999..7232
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7417..7461
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7746..7863
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7937..8286
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    7945..7963
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7981..7999
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8017..8035
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8053..8071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8089..8107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8125..8143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8161..8179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8225..8249
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    9633..10206
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9800..9817)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10027..10046
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10236..10934
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     misc_feature    10949..11537
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11002..11022)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11420..11431)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gctgcgtctc aagcatacac gataccgtcg acctcgaggg aattaattcg agctcggtac
       61 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
      121 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
      181 ggtttctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      241 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      301 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      361 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
      421 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
      481 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
      541 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
      601 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
      661 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
      721 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
      781 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
      841 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
      901 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
      961 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     1021 cttacagaca agctgtgacc atctccggga gctgcatgtg tcagaggttt tcaccgtcat
     1081 caccgaaacg cgcgagatga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1141 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg ccgagcggcc
     1201 gcacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1261 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1321 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1381 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1441 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1501 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1561 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1621 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1681 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1741 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1801 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1861 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1921 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgacagg tggcactttt
     1981 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2041 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2101 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2161 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2221 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2281 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2341 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2401 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2461 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2521 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2581 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2641 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2701 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2761 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcccgc
     2821 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2881 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2941 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3001 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tcgcggccgc
     3061 caatttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3121 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3181 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     3241 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     3301 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3361 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3421 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3481 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3541 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3601 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     3661 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     3721 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     3781 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     3841 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     3901 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
     3961 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     4021 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     4081 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
     4141 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
     4201 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
     4261 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
     4321 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     4381 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     4441 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
     4501 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
     4561 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
     4621 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
     4681 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     4741 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     4801 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     4861 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     4921 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     4981 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     5041 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     5101 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
     5161 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
     5221 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
     5281 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     5341 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     5401 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5461 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5521 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     5581 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
     5641 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     5701 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     5761 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     5821 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     5881 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     5941 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     6001 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     6061 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     6121 catataagca gctgcttttt gcctgtactg ggactctctg gttagaccag atctgagcct
     6181 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6241 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6301 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     6361 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     6421 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     6481 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     6541 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     6601 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     6661 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     6721 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     6781 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     6841 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     6901 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     6961 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     7021 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     7081 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     7141 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     7201 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     7261 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     7321 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     7381 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     7441 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     7501 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     7561 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     7621 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     7681 gacagatcca ttcgattagt gaacggatct cgacggtatc gccaaatggc agtattcatc
     7741 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     7801 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     7861 tttcgggttt attacaggga cagcagagat ccagtttggc cctctgatga gacgaacggc
     7921 cctttcgtct tcactcgagt ttactcccta tcagtgatag agaacgtatg aagagtttac
     7981 tccctatcag tgatagagaa cgtatgcaga ctttactccc tatcagtgat agagaacgta
     8041 taaggagttt actccctatc agtgatagag aacgtatgac cagtttactc cctatcagtg
     8101 atagagaacg tatctacagt ttactcccta tcagtgatag agaacgtata tccagtttac
     8161 tccctatcag tgatagagaa cgtatgtcga ggtaggcgtg tacggtgggc gcctataaaa
     8221 gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt
     8281 atacttacgc gtctgcagga tatcaagctt gcggtaccgc gggcccggga tctatgcccc
     8341 tgttgtggtt gcgcgggttt ctcctggctt cctgttggat aatagtgagg agctccccca
     8401 ccccgggcag cgagggacac ggttccgctc ccgattgccc gtcatgcgct ctggctactc
     8461 tccctaagga cggtcctaat tctcaacctg aaatggtcga ggcagtcaag aagcacatac
     8521 tcaatatgct gcatctgaag aaaagacctg acgtgaccca gcctgtgcca aaagcagcgc
     8581 tattgaacgc aattcggaaa ctgcatgtgg gcaaagttgg cgaaaacggc tacgtcgaaa
     8641 tagaggacga tatcggaaga agggccgaga tgaacgaact catggaacaa actagtgaga
     8701 ttataacctt cgccgaatct ggcactgccc gaaagacact ccacttcgag atctctaaag
     8761 aaggtagcga tctctccgtt gttgaaagag ccgaagtgtg gctgttcctc aaagtgccca
     8821 aagctaatcg gacgcggact aaagtgacca ttcggttgtt ccaacaacag aaacaccctc
     8881 aaggaagtct ggacaccggt gacgaggcag aagaaatggg actgaaaggg gaaaggagtg
     8941 agctgctact cagcgaaaag gtcgtagacg cacgcaagtc tacatggcat atctttccag
     9001 tcagttccag catacaacgg ctcctagatc aaggcaaatc cagtctcgat gtgcgaatcg
     9061 cttgcgagca atgtcaagaa tcaggcgcct ctctggttct tctcggtaag aagaagaaga
     9121 aggaagttga tggcgacggg aagaagaagg acggatctga tggtggactt gaggaagaaa
     9181 aggaacagag ccataggccg tttctgatgt tgcaagccag gcagtccgag gatcatccgc
     9241 atcgcaggcg caggagaggg cttgaatgcg atggcaaggt caatatttgt tgtaagaaac
     9301 aattcttcgt gtcctttaaa gatattgggt ggaacgattg gatcatcgca ccatccggat
     9361 atcacgcaaa ttattgcgaa ggggaatgtc catctcatat cgcagggacc agcggtagtt
     9421 ccttgagctt tcattcaact gttattaatc attatcgtat gcgagggcac agtcctttcg
     9481 ctaacctcaa atcctgttgc gtccccacaa agctccgtcc tatgtctatg ctctactacg
     9541 acgacggcca aaacatcata aagaaggata tacaaaatat gatagtagag gagtgcggtt
     9601 gttctggttc ttaagatcct tgacttgcgg cccccctctc cctccccccc ccctaacgtt
     9661 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     9721 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     9781 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     9841 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     9901 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     9961 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
    10021 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
    10081 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
    10141 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
    10201 tgataaatga gcgctggcgg accaccatgg gatccagcga gctgattaag gagaacatgc
    10261 acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg
    10321 gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc
    10381 ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca
    10441 tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat
    10501 gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc
    10561 tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg
    10621 gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgaaacg ctgtaccccg
    10681 ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc
    10741 tgatcgcaaa catcaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc
    10801 ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgaaacct
    10861 acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc
    10921 acaagcttaa ttaatggcgc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
    10981 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
    11041 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
    11101 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
    11161 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
    11221 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
    11281 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
    11341 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
    11401 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
    11461 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
    11521 ttgggccgcc tccccgcat
//
