LOCUS       sequence-448304-        9436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyback backbone for non-fluorescent GFP tether and H2B-IRFP 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Yamada T, Trentesaux C, Brunger JM, Xiao Y, Stevens AJ, Martyn I, 
            Kasparek P, Shroff NP, Aguilar A, Bruneau BG, Boffelli D, Klein OD, 
            Lim WA
  TITLE     Synthetic organizer cells guide development via spatial and 
            biochemical instructions.
  JOURNAL   Cell. 2024 Dec 11:S0092-8674(24)01323-0. doi: 
            10.1016/j.cell.2024.11.017.
  PUBMED    39706189
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Dec 11:S0092-8674(24)01323-0. doi: 10.1016/j.cell.2024.11.017."
COMMENT     Sequence Label: aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA
FEATURES             Location/Qualifiers
     source          1..9436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      184..772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     673..692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     884..903
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        884..902
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   932..966
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1471..1774
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1776..2051
                     /label=chicken beta-actin promoter
     intron          2052..3068
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2992..3014
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3076..3095
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4062..4118
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4119..4496
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4509..4518
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4515..5462
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     primer_bind     complement(5491..5510)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5545..5564
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6718..6921
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6872..6892
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      6943..6952
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6949..7977
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             6949..7977
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     primer_bind     complement(7989..8006)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7995..8202
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8409..8471
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(8522..8540)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(8580..9395)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8580..9395)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9303..9322
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9387..9406
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
       61 cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagtca tgaccaaaat
      121 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      181 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      241 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      301 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
      361 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      421 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      481 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      541 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      601 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      661 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      721 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      781 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      841 tgcgttatcc cctgattctg tggataaccg tgcggccgcc aattaatacg actcactata
      901 gggcgaattg gggcgcgcca ttctagatta accctagaaa gatagtctgc gtaaaattga
      961 cgcatgcatt cttgaaatat tgctctctct ttctaaatag cgcgaatccg tcgctgtgca
     1021 tttaggacat ctcagtcgcc gcttggagct cccgtgaggc gtgcttgtca atgcggtaag
     1081 tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa tgacgcatga ttatctttta
     1141 cgtgactttt aagatttaac tcatacgata attatattgt tatttcatgt tctacttacg
     1201 tgataactta ttatatatat attttcttgt tatagatatc aactagaatg ctagcatggg
     1261 cccagatctc catgggccct gaattcgcat ctagactgat gagacgtggt agagccacaa
     1321 acagccggta caagcaacga tctccaggac catctgaatc atgcgcggat gacacgaact
     1381 cacgacggcg atcacagaca ttaacccaca gtacagacac tgcgacaacg tggcaattcg
     1441 tcgcaataca acgccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1501 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1561 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1621 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     1681 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     1741 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     1801 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     1861 attttgtgca gcgatggggg cggggggggg gggggggcgc gccagggggg gggggggggg
     1921 gggagggggg gggsggggsg aggcggagag gtgcggcggc agccaatcag agcggcgcgc
     1981 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
     2041 cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
     2101 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     2161 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
     2221 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
     2281 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
     2341 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
     2401 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
     2461 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac
     2521 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc
     2581 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc
     2641 cgggcggggc ggggccgccy cgggccgggg rgggctcggg ggrggggcgc ggcggccccc
     2701 ggagtgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
     2761 gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
     2821 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
     2881 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
     2941 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
     3001 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     3061 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     3121 tcgccactat ggaaaccgac accctcttgc tctgggtcct gctgctgtgg gttccgggta
     3181 gtaccgggga cgtttctaag ggtgaagaat tgttcaccgg agttgtgccc atcctggttg
     3241 agctggacgg tgacgttaac ggacacaagt tctcagtgag cggcgaagga gagggcgacg
     3301 ctacgtacgg taaactgacg ttgaagttca tctgtacaac aggaaagctg ccagtgccct
     3361 ggcctacact cgtgaccacc ctcaccttcg gagtccagtg cttctcccgg taccccgacc
     3421 acatgaaaca gcacgatttc ttcaagtccg cgatgcctga gggctatgtt caggaacgca
     3481 ccatattctt caaggacgac ggtaactata aaaccagggc tgaagttaag ttcgaggggg
     3541 acacgttggt aaatagaatt gagttgaaag gaatcgattt caaagaagac ggcaatattt
     3601 tgggccacaa gctggagtac aactacaaca gccacaacgt gtatattatg gctgataagc
     3661 agaagaacgg catcaaggtg aacttcaaaa tccgacacaa tatcgaggat ggctctgtgc
     3721 aactggccga ccattaccag caaaacacgc ctatcggcga cggtccggtt ttgctgccag
     3781 ataaccacta cctctccacg caatcagccc tctccaagga tcctaacgaa aaaagggacc
     3841 atatggttct gcttgaattc gtgacggctg ctgggatcac cctgggaatg gatgagctgt
     3901 ataagaacgc cgtgggccag gatacgcagg aggtgatcgt cgtgccccac agcttgccct
     3961 tcaaggtcgt ggttattagt gctatcctgg cgttggtggt gctgactatt atctccctta
     4021 tcatacttat catgctgtgg caaaaaaaac cccgaggatc cgctactaac ttcagcctgc
     4081 tgaagcaggc tggcgacgtg gaggagaacc ctggacctat gccagagcca gcgaagtctg
     4141 ctcccgcccc gaaaaagggc tccaagaagg cggtgactaa ggcgcagaag aaaggcggca
     4201 agaagcgcaa gcgcagccgc aaggagagct attccatcta tgtgtacaag gttctgaagc
     4261 aggtccaccc tgacaccggc atttcgtcca aggccatggg catcatgaat tcgtttgtga
     4321 acgacatttt cgagcgcatc gcaggtgagg cttcccgcct ggcgcattac aacaagcgct
     4381 cgaccatcac ctccagggag atccagacgg ccgtgcgcct gctgctgcct ggggagttgg
     4441 ccaagcacgc cgtgtccgag ggtactaagg ccatcaccaa gtacaccagc gctaaggatc
     4501 caccggtcgc caccatggta gcaggtcatg cctctggcag ccccgcattc gggaccgcct
     4561 ctcattcgaa ttgcgaacat gaagagatcc acctcgccgg ctcgatccag ccgcatggcg
     4621 cgcttctggt cgtcagcgaa catgatcatc gcgtcatcca ggccagcgcc aacgccgcgg
     4681 aatttctgaa tctcggaagc gtactcggcg ttccgctcgc cgagatcgac ggcgatctgt
     4741 tgatcaagat cctgccgcat ctcgatccca ccgccgaagg catgccggtc gcggtgcgct
     4801 gccggatcgg caatccctct acggagtact gcggtctgat gcatcggcct ccggaaggcg
     4861 ggctgatcat cgaactcgaa cgtgccggcc cgtcgatcga tctgtcaggc acgctggcgc
     4921 cggcgctgga gcggatccgc acggcgggtt cactgcgcgc gctgtgcgat gacaccgtgc
     4981 tgctgtttca gcagtgcacc ggctacgacc gggtgatggt gtatcgtttc gatgagcaag
     5041 gccacggcct ggtattctcc gagtgccatg tgcctgggct cgaatcctat ttcggcaacc
     5101 gctatccgtc gtcgactgtc ccgcagatgg cccggcagct gtatgtgaga cagcgcgtca
     5161 gagtgctggt cgatgtcacc tatcagccag tgcctctgga gcctagactg tcgccactga
     5221 ccgggagaga tctcgacatg tcgggctgct tcctgagatc gatgtcgccg tgccatctgc
     5281 agttcctgaa ggacatgggc gtgcgcgcca ccctggcggt gtcgctggtg gtcggcggca
     5341 agctgtgggg cctggttgtc tgtcaccatt atctgccgcg cttcatccgt ttcgagctgc
     5401 gggcgatctg caaacggctc gccgaaagga tcgcgacgcg gatcaccgcg cttgagagct
     5461 aatggcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     5521 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     5581 ataagctgca ataaacgctg gaaatctgct cgtcagtggt gctcacactg acgaatcatg
     5641 tacagatcat accgatgact gcctggcgac tcacaactaa gcaagacagc cggaaccagc
     5701 gccggcgaac accactgcat atatggcata tcacaacagt ccacgtctca agcagttaca
     5761 gagatgttac gaaccactag tgcactgcag atcgaattcg agttggcgcg cctgtcattc
     5821 taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc tgcaggctca
     5881 aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc
     5941 tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc
     6001 gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg
     6061 ggagggggct gtccccgtga gctcccaggc gcgcctgtca ttctaaatct ctctttcagc
     6121 ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg
     6181 ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc
     6241 ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc
     6301 cccctagcgg gggagggacg taattacatc cctgggggct ttgggggggg gctgtccccg
     6361 tgagctcaat aaaagagccc actacagcgg tgggctctat ggggtaccta cgtaggtaca
     6421 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     6481 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     6541 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     6601 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     6661 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     6721 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     6781 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     6841 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     6901 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tagccaccat gggtaaaaag
     6961 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtgtcc
     7021 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     7081 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     7141 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattt
     7201 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     7261 cctgaaaccg aactgcccgc tgttctgcaa ccggtcgcgg aggccatgga tgcgatcgct
     7321 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     7381 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     7441 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     7501 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     7561 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     7621 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     7681 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     7741 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     7801 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     7861 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     7921 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataactc
     7981 gagcgacgcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     8041 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     8101 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     8161 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatgag
     8221 tatcgatact agttaaaagt tttgttactt tatagaagaa attttgagtt tttgtttttt
     8281 tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg
     8341 taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg
     8401 ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac aatatgatta
     8461 tctttctagg gttaatctag tatacgcgta tgcggccgct taattaatcc agcttttgtt
     8521 ccctttagtg agggttaatt ccgagcggcc gcgtgttaca accaattaac caattctgat
     8581 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     8641 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     8701 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     8761 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     8821 gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag
     8881 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     8941 gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     9001 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     9061 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     9121 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     9181 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     9241 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     9301 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     9361 ctggagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     9421 taagcagaca gtttta
//
