LOCUS       sequence-448305-        9901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for iRFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Yamada T, Trentesaux C, Brunger JM, Xiao Y, Stevens AJ, Martyn I, 
            Kasparek P, Shroff NP, Aguilar A, Bruneau BG, Boffelli D, Klein OD, 
            Lim WA
  TITLE     Synthetic organizer cells guide development via spatial and 
            biochemical instructions.
  JOURNAL   Cell. 2024 Dec 11:S0092-8674(24)01323-0. doi: 
            10.1016/j.cell.2024.11.017.
  PUBMED    39706189
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Dec 11:S0092-8674(24)01323-0. doi: 10.1016/j.cell.2024.11.017."
COMMENT     Sequence Label: aTY171_pPGK1_iRFP_WPRE
FEATURES             Location/Qualifiers
     source          1..9901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..515
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(51..74)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     448..467
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             545..1477
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     misc_feature    1503..2091
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1556..1576)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1974..1985)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2113..2129)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2114..2130)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2220..2453
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2478..2496)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2479..2496)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2786..2805)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3019..3038)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3138..3160
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3198..3216)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(3395..3983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3475..3494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(4197..4216)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4208..5023
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             4208..5023
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4281..4300)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5198..5215
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5285..5305)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5290..5619
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5470..5605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6753..6818
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6948..6968
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7393..7527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7430..7449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7484..7503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7695..8328
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8375..8500
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8997..9230
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9415..9459
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9744..9861
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctgatgagac gaacggggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
       61 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
      121 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
      181 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
      241 aaatggaagt agcatgtctc actaggctcg tgcagatgga cagcaccgct gagcaatgga
      301 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
      361 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
      421 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
      481 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttgtcgacgc
      541 cactatggcg cgtaaggtcg atctcacctc ctgcgatcgc gagccgatcc acatccccgg
      601 cagcattcag ccgtgcggct gcctgctagc ctgcgacgcg caggcggtgc ggatcacgcg
      661 cattacggaa aatgccggcg cgttctttgg acgcgaaact ccgcgggtcg gtgagctact
      721 cgccgattac tttggcgaaa ccgaagccca tgcgctgcgc aacgcactgg cgcagtcctc
      781 cgatccaaag cgaccggcgc tgatcttcgg ttggcgcgac ggcctgaccg gccgcacctt
      841 cgacatctca ctgcatcgcc atgacggtac atcgatcatc gagttcgagc ctgcggcggc
      901 cgaacaggcc gacaatccgc tgcggctgac gcggcagatc atcgcgcgca ccaaagaact
      961 gaagtcgctc gaagagatgg ccgcacgggt gccgcgctat ctgcaggcga tgctcggcta
     1021 tcaccgcgtg atgttgtacc gcttcgcgga cgacggctcc gggatggtga tcggcgaggc
     1081 gaagcgcagc gacctggaga gctttctcgg tcagcacttt ccggcgtcgc tggtcccgca
     1141 gcaggcgcgg ctactgtact tgaagaacgc gatccgcgtg gtatcggatt cgcgcggcat
     1201 cagcagccgg atcgtgcccg agcacgacgc ctccggcgcc gcgctcgatc tgtcgttcgc
     1261 gcacctgcgc agcatctcgc cctgccatct cgaatttctg cggaacatgg gcgtcagcgc
     1321 ctcgatgtcg ctgtcgatca tcattgacgg cacgctatgg ggattgatca tctgtcatca
     1381 ttacgagccg cgtgccgtgc cgatggcgca gcgcgtcgcg gccgaaatgt tcgccgactt
     1441 cttatcgctg cacttcaccg ccgcccacca ccaacgcgga tccggatcct aagcttatcg
     1501 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     1561 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     1621 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     1681 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     1741 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     1801 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     1861 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     1921 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     1981 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     2041 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catgctgcgt
     2101 ctcaagcata cacgataccg tcgacctcga gggaattaat tcgagctcgg tacctttaag
     2161 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     2221 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtttct
     2281 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     2341 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     2401 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     2461 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     2521 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     2581 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     2641 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     2701 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     2761 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     2821 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     2881 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     2941 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     3001 cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3061 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3121 acaagctgtg accatctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3181 acgcgcgaga tgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3241 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgccgagcg gccgcacggt
     3301 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3361 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3421 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3481 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3541 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3601 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3661 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3721 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3781 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3841 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3901 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3961 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4021 agtggaacga aaactcacgt taagggattt tggtcatgac tgatccttca actcagcaaa
     4081 agttcgattt attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca
     4141 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     4201 gggtgttatg agccatattc aacgggaaac gtcttgctcc aggccgcgat taaattccaa
     4261 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     4321 gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     4381 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     4441 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     4501 cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     4561 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     4621 ttgtcctttt aacagcgatc gcgtatttcg cctcgctcag gcgcaatcac gaatgaataa
     4681 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     4741 ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     4801 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     4861 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     4921 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     4981 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     5041 gttgtaacac gcggccgcca attttttacg gttcctggcc ttttgctggc cttttgctca
     5101 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5161 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5221 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5281 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     5341 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5401 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     5461 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5521 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5581 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     5641 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     5701 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     5761 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     5821 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     5881 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     5941 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     6001 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     6061 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     6121 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     6181 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     6241 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     6301 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     6361 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     6421 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     6481 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     6541 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     6601 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     6661 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     6721 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     6781 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     6841 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     6901 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     6961 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     7021 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     7081 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     7141 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     7201 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     7261 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     7321 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     7381 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7441 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7501 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     7561 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     7621 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     7681 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     7741 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     7801 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     7861 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     7921 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     7981 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     8041 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     8101 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg actctctggt
     8161 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8221 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8281 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     8341 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     8401 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     8461 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     8521 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     8581 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     8641 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     8701 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     8761 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     8821 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     8881 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     8941 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     9001 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     9061 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     9121 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     9181 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     9241 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     9301 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     9361 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     9421 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     9481 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     9541 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     9601 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     9661 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
     9721 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     9781 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     9841 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggccc
     9901 t
//
