LOCUS       sequence-448307-        9673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for BFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Yamada T, Trentesaux C, Brunger JM, Xiao Y, Stevens AJ, Martyn I, 
            Kasparek P, Shroff NP, Aguilar A, Bruneau BG, Boffelli D, Klein OD, 
            Lim WA
  TITLE     Synthetic organizer cells guide development via spatial and 
            biochemical instructions.
  JOURNAL   Cell. 2024 Dec 11:S0092-8674(24)01323-0. doi: 
            10.1016/j.cell.2024.11.017.
  PUBMED    39706189
REFERENCE   2  (bases 1 to 9673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Dec 11:S0092-8674(24)01323-0. doi: 10.1016/j.cell.2024.11.017."
COMMENT     Sequence Label: aTY173_pPGK1_mTagBFP2_WPRE
FEATURES             Location/Qualifiers
     source          1..9673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..515
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(51..74)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     448..467
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     misc_feature    1275..1863
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1328..1348)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1746..1757)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1885..1901)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1886..1902)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1992..2225
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2250..2268)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2251..2268)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2558..2577)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2791..2810)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2910..2932
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2970..2988)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(3167..3755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3247..3266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(3969..3988)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3980..4795
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3980..4795
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4053..4072)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4970..4987
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5057..5077)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5062..5391
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5242..5377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6525..6590
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6720..6740
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7165..7299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7202..7221)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7256..7275
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7467..8100
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8147..8272
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8769..9002
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9187..9231
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9516..9633
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctgatgagac gaacggggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
       61 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
      121 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
      181 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
      241 aaatggaagt agcatgtctc actaggctcg tgcagatgga cagcaccgct gagcaatgga
      301 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
      361 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
      421 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
      481 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttgtcgacgc
      541 cactatggtt tcaaagggtg aagaattgat caaagaaaac atgcatatga aattatatat
      601 ggaaggtaca gttgacaacc atcattttaa gtgtacatct gaaggagaag gtaagcctta
      661 cgagggaact caaactatga gaataaaagt cgtagaaggt ggaccattgc cttttgcttt
      721 tgatatctta gcaacatctt tcttatatgg ttctaaaacc tttattaacc atactcaagg
      781 tatccctgat ttcttcaaac aatctttccc tgaaggattt acttgggaga gggttacaac
      841 atatgaggat ggtggtgttt tgacagcaac ccaggataca tcattgcagg atggatgttt
      901 gatttataat gtaaagatta gaggagtaaa ttttacttct aacggtcctg taatgcaaaa
      961 gaagacttta ggttgggaag ctttcaccga aacattatat ccagcagacg gaggtttaga
     1021 gggaagaaac gatatggctt tgaagttagt cggtggttca cacttaatag ctaacgccaa
     1081 gactacttac aggtctaaga aaccagcaaa gaatttgaaa atgccaggag tttattatgt
     1141 tgactacaga ttggaaagga tcaaggaggc caataacgag acatatgtag agcagcatga
     1201 agtcgccgtc gccagatatt gcgacttacc ttctaagttg ggtcataaat tgaatggatc
     1261 ctaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     1321 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     1381 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     1441 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     1501 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     1561 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     1621 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     1681 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     1741 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     1801 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     1861 cgcatgctgc gtctcaagca tacacgatac cgtcgacctc gagggaatta attcgagctc
     1921 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     1981 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     2041 tactgggttt ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2101 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2161 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2221 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
     2281 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
     2341 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
     2401 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
     2461 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
     2521 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     2581 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     2641 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     2701 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     2761 aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2821 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     2881 atccgcttac agacaagctg tgaccatctc cgggagctgc atgtgtcaga ggttttcacc
     2941 gtcatcaccg aaacgcgcga gatgaaaggg cctcgtgata cgcctatttt tataggttaa
     3001 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgccgag
     3061 cggccgcacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3121 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3181 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3241 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3301 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3361 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3421 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3481 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3541 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3601 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3661 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3721 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3781 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg actgatcctt
     3841 caactcagca aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg
     3901 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     3961 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct ccaggccgcg
     4021 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     4081 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct
     4141 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     4201 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     4261 atggttactc accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc
     4321 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     4381 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgcctcgctc aggcgcaatc
     4441 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     4501 tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt
     4561 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     4621 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     4681 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     4741 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     4801 attggttaat tggttgtaac acgcggccgc caatttttta cggttcctgg ccttttgctg
     4861 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4921 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     4981 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     5041 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5101 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5161 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5221 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5281 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5341 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
     5401 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
     5461 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     5521 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     5581 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     5641 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     5701 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     5761 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     5821 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     5881 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     5941 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     6001 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     6061 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     6121 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     6181 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     6241 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     6301 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
     6361 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
     6421 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
     6481 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     6541 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     6601 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     6661 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     6721 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     6781 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     6841 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     6901 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     6961 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     7021 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     7081 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     7141 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     7201 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7261 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     7321 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     7381 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     7441 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     7501 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     7561 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     7621 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     7681 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     7741 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     7801 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     7861 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     7921 ggactctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7981 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8041 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8101 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     8161 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     8221 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     8281 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     8341 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     8401 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     8461 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     8521 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     8581 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
     8641 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     8701 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     8761 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     8821 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     8881 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     8941 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     9001 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     9061 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     9121 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     9181 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     9241 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     9301 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     9361 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     9421 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     9481 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     9541 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     9601 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     9661 ccagtttggc cct
//
