LOCUS       sequence-448308-       12650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for Pcad expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12650)
  AUTHORS   Yamada T, Trentesaux C, Brunger JM, Xiao Y, Stevens AJ, Martyn I, 
            Kasparek P, Shroff NP, Aguilar A, Bruneau BG, Boffelli D, Klein OD, 
            Lim WA
  TITLE     Synthetic organizer cells guide development via spatial and 
            biochemical instructions.
  JOURNAL   Cell. 2024 Dec 11:S0092-8674(24)01323-0. doi: 
            10.1016/j.cell.2024.11.017.
  PUBMED    39706189
REFERENCE   2  (bases 1 to 12650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Dec 11:S0092-8674(24)01323-0. doi: 10.1016/j.cell.2024.11.017."
COMMENT     Sequence Label: aTY50_pPGK1_Pcad_IRES_PuroR_WPRE
FEATURES             Location/Qualifiers
     source          1..12650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..515
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(51..74)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     448..467
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     misc_feature    3038..3611
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3205..3222)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3432..3451
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3641..4237
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4134..4154
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4252..4840
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4305..4325)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4723..4734)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4862..4878)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4863..4879)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4969..5202
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5227..5245)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5228..5245)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5535..5554)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5768..5787)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5887..5909
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5947..5965)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(6144..6732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6224..6243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6946..6965)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6957..7772
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6957..7772
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7030..7049)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7947..7964
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8034..8054)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8039..8368
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8219..8354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9502..9567
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9697..9717
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10142..10276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10179..10198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10233..10252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10444..11077
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11124..11249
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11746..11979
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12164..12208
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12493..12610
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctgatgagac gaacggggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
       61 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
      121 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
      181 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
      241 aaatggaagt agcatgtctc actaggctcg tgcagatgga cagcaccgct gagcaatgga
      301 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
      361 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
      421 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
      481 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttgtcgacgc
      541 cactatggag cttcttagtg ggcctcacgc cttcctgctc ctcctgctcc aggtttgctg
      601 gctacgcagc gttgtctccg agccctaccg agcgggcttc atcggggagg ctggagtgac
      661 cttggaggtg gaaggaactg acctggagcc gagccaagtt ctggggaaag tagccttggc
      721 tggacagggc atgcaccatg cagacaatgg agacatcatt atgctgacta gggggacagt
      781 tcagggaggg aaggatgcga tgcactcccc acccacccgc atcttaagaa gacgaaagag
      841 agagtgggtg atgccaccaa tattcgtccc cgagaatggc aagggtccct tccctcagag
      901 gctgaatcag ctcaaatcta ataaggacag aggcaccaag attttctaca gcatcacagg
      961 gcctggcgca gacagtcccc ccgaaggagt cttcaccata gagaaggagt cgggctggct
     1021 gttgttgcat atgccactgg acagggagaa gattgtcaag tacgagcttt atggccacgc
     1081 tgtatctgag aatggtgcct ctgtagagga gcccatgaac atatccatca ttgtgacaga
     1141 ccagaatgac aacaagccca agttcactca agacaccttc agagggagtg ttctggaggg
     1201 agtaatgcct ggcacttctg tgatgcaggt gacagccaca gatgaggacg atgctgtcaa
     1261 cacttacaat ggggtggtgg cttactccat ccatagccaa gagccgaagg agccacacga
     1321 cctcatgttc accatccata aaagcacggg aaccattagc gtcatatcca gtggcctgga
     1381 ccgagagaaa gtccctgagt acagactgac cgtccaggcc acagacatgg atggagaggg
     1441 ctctaccacg acggcagagg ccgttgtgca aatccttgat gccaacgata acgctccgga
     1501 gtttgagccg cagaagtatg aggcttgggt gcctgagaac gaagtgggcc atgaggtaca
     1561 gaggctgaca gtgactgatc tcgatgtccc caactcgcca gcgtggcgtg ccacctacca
     1621 catcgtggga ggtgatgatg gggaccattt caccatcacc actcacccag aaaccaacca
     1681 aggcgtcctg acaaccaaga agggtttgga ttttgaggct caggaccaac acaccctgta
     1741 tgtagaagtg accaacgagg ctccctttgc agtgaagctc ccgacagcca ctgccaccgt
     1801 ggtggtccat gtgaaagatg tcaacgaagc ccctgtgttt gttccacctt ccaaggtcat
     1861 tgaggcccag gaaggcatct ctattgggga actggtctgc atctataccg cacaggaccc
     1921 agacaaggag gaccagaaga tcagctacac catctcgaga gatccagcca actggcttgc
     1981 tgtggaccca gacagtggtc agataactgc cgcaggcatc ttggatcgtg aggacgagca
     2041 gtttgtgaaa aacaatgtct acgaagtcat ggttttggcc acagacagtg gaaaccctcc
     2101 caccaccggc actgggaccc tcctgcttac acttactgac atcaacgacc atggcccgat
     2161 ccctgaaccc aggcagatca tcatctgtaa ccaaagccct gtgcctcaag tgctgaacat
     2221 cactgacaag gacctgtccc ccaactcctc ccctttccag gcccagctaa cacatgactc
     2281 agatatctac tggatggcag aagtcagcga gaaaggagac accgtggcct tgtccctgaa
     2341 gaagttcctg aaacaagaca cgtatgactt gcatctttct ttgtctgacc atggcaacag
     2401 ggaacagcta accatgatca gggccactgt gtgtgactgc catggccaag tgttcaatga
     2461 ctgccccaga ccctggaagg gtggtttcat cctccccatc ctgggtgctg tcctggcact
     2521 gctgaccctt ctactggcac tcctcctgtt ggtgaggaag aagaggaagg tcaaagagcc
     2581 ccttctgctc ccagaagatg acacgcgaga caatgtcttc tattatggag aagagggtgg
     2641 tggtgaagag gaccaggact atgacatcac ccaactccac cggggactgg aggccaggcc
     2701 tgaggtggtt ctccgaaacg atgtagtgcc aaccttcatc cccaccccca tgtaccgacc
     2761 ccggcccgcc aacccagatg aaatcgggaa cttcatcatc gagaacctga aggctgccaa
     2821 cactgaccct actgccccgc cctacgactc cctgctggtt tttgactacg agggcagcgg
     2881 ctctgatgcc gcctccctga gctccctcac cacctccgcc tccgaccagg atcaggacta
     2941 caactacctt aacgagtggg gaagtcgatt caagaaactg gcggacatgt atggtggcgg
     3001 tgaggatgac ggttcttaag atccttgact tgcggccccc ctctccctcc ccccccccta
     3061 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     3121 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     3181 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     3241 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     3301 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     3361 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     3421 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     3481 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     3541 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     3601 cacgatgata aatgagcgct ggcggaccac catgggatcc accgagtaca agcccacggt
     3661 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     3721 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     3781 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     3841 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     3901 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     3961 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4021 cggcgtgtcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4081 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acatccgcgc cccgcaacct
     4141 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     4201 gcgcacctgg tgcatgaccc gcaagcccgg tgcctaatgg cgcttatcga taatcaacct
     4261 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4321 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4381 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4441 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4501 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4561 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4621 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4681 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4741 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4801 cctcagacga gtcggatctc cctttgggcc gcctccccgc atgctgcgtc tcaagcatac
     4861 acgataccgt cgacctcgag ggaattaatt cgagctcggt acctttaaga ccaatgactt
     4921 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     4981 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtttctc tggttagacc
     5041 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5101 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5161 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc
     5221 gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt
     5281 gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa
     5341 gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt
     5401 ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt
     5461 catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     5521 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     5581 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     5641 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     5701 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca
     5761 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     5821 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5881 ccatctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagat
     5941 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6001 agacgtcagg tggcactttt cggggaaatg tgccgagcgg ccgcacggtt atccacagaa
     6061 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6121 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6181 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6241 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6301 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6361 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6421 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6481 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6541 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6601 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6661 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6721 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6781 aactcacgtt aagggatttt ggtcatgact gatccttcaa ctcagcaaaa gttcgattta
     6841 ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     6901 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     6961 gccatattca acgggaaacg tcttgctcca ggccgcgatt aaattccaac atggatgctg
     7021 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     7081 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     7141 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     7201 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     7261 ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     7321 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     7381 acagcgatcg cgtatttcgc ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     7441 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     7501 tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     7561 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     7621 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     7681 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     7741 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacacg
     7801 cggccgccaa ttttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7861 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7921 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7981 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     8041 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8101 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8161 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8221 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8281 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8341 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     8401 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     8461 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     8521 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     8581 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     8641 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     8701 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     8761 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     8821 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     8881 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     8941 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     9001 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     9061 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     9121 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     9181 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     9241 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     9301 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     9361 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     9421 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     9481 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     9541 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     9601 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     9661 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     9721 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     9781 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     9841 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     9901 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     9961 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
    10021 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
    10081 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    10141 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    10201 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10261 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    10321 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    10381 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    10441 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
    10501 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
    10561 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
    10621 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
    10681 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
    10741 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
    10801 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
    10861 gatcctgcat ataagcagct gctttttgcc tgtactggga ctctctggtt agaccagatc
    10921 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    10981 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    11041 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
    11101 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
    11161 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
    11221 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
    11281 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
    11341 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
    11401 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
    11461 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
    11521 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
    11581 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    11641 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    11701 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    11761 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    11821 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    11881 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    11941 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    12001 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    12061 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    12121 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    12181 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    12241 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    12301 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    12361 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
    12421 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc aaatggcagt
    12481 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
    12541 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
    12601 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggccct
//
