LOCUS       sequence-448322-        8614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpressing N-terminus truncation of NRBP1 with C-terminal FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Xiao YX, Lee SY, Aguilera-Uribe M, Samson R, Au A, Khanna Y, Liu Z, 
            Cheng R, Aulakh K, Wei J, Farias AG, Reilly T, Birkadze S, Habsid A,
            Brown KR, Chan K, Mero P, Huang JQ, Billmann M, Rahman M, Myers C, 
            Andrews BJ, Youn JY, Yip CM, Rotin D, Derry WB, Forman-Kay JD, Moses
            AM, Pritisanac I, Gingras AC, Moffat J
  TITLE     The TSC22D, WNK, and NRBP gene families exhibit functional buffering
            and evolved with Metazoa for cell volume regulation.
  JOURNAL   Cell Rep. 2024 Jul 23;43(7):114417. doi: 
            10.1016/j.celrep.2024.114417. Epub 2024 Jul 8.
  PUBMED    38980795
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-23"; volume: "43(7):114417. doi"; pages: " 
            10.1016/j.celrep.2024.114417. Epub 2024 Jul 8"
COMMENT     Sequence Label: pLJM1-NRBP1-Nterm-C-FLAG
FEATURES             Location/Qualifiers
     source          1..8614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    103..224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(114..133)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     168..187
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             296..529
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(657..1256)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(740..760)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1237..1256
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1278..1788)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1341..1359)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    1837..1954
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1999..2022)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(3317..3520)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3346..3366)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(3809..3829)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(4127..4171)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4356..4589
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5082..5207
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5254..5434
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5435..5661)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5689..5709)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5689..5707)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5728..5744)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5728..5744)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5741..5763)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5752..5768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5776..5782,5783..5800,5801..5806))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5821..5842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5959..5976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6130..6718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6210..6229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6889..7680,7681..7749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7512..7531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7750..7854)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7880..8335)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8017..8038)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8229..8248
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8462..8484
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8476..8493
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8477..8493
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8503..8522
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8503..8521
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      join(8542..8614,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggatagctag agccagacat gataagatac attgatgagt
      121 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      181 ctattgcttt atttgtaacc attataagct gcaataaaca agttcctctc actctctgat
      241 attcatttct ttgcaagtta taaatactga ataataagat gacatgaact actactgcta
      301 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
      361 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
      421 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
      481 caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
      541 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc
      601 gatgcatggg gtcgtgcgct cctttcggtc gggcgctgcg ggtcgtgggg cgggcgtcag
      661 gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg
      721 gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg
      781 aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac ggcgctgccc
      841 agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc
      901 ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag ccgggaaccg
      961 ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc gacgctctcc
     1021 ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg
     1081 agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat gtggcggtcc
     1141 ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg
     1201 gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggta
     1261 agctccggtg gatccccctg gggagagagg tcggtgattc ggtcaacgag ggagccgact
     1321 gccgacgtgc gctccggagg cttgcagaat gcggaacacc gcgcgggcag gaacagggcc
     1381 cacactaccg ccccacaccc cgcctcccgc accgcccctt cccggccgct gctctcggcg
     1441 cgccctgctg agcagccgct attggccaca gcccatcgcg gtcggcgcgc tgccattgct
     1501 ccctggcgct gtccgtctgc gagggtacta gtgagacgtg cggcttccgt ttgtcacgtc
     1561 cggcacgccg cgaaccgcaa ggaaccttcc cgacttaggg gcggagcagg aagcgtcgcc
     1621 ggggggccca caagggtagc ggcgaagatc cgggtgacgc tgcgaacgga cgtgaagaat
     1681 gtgcgagacc cagggtcggc gccgctgcgt ttcccggaac cacgcccaga gcagccgcgt
     1741 ccctgcgcaa acccagggct gccttggaaa aggcgcaacc ccaaccccct cgagccgcgg
     1801 ccaaagtgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg
     1861 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
     1921 actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct
     1981 cgaggtcgag aattcctact tgtcatcgtc gtccttgtag tctgatgtca cctcctcctg
     2041 ctgtggctgc tggggccgag gcagcccaaa ggctgtcaga ggatagatcc cattcctgac
     2101 atcttcaagg aatttatcta attccagagc tggtgactga gagtacaaag tctgaactgg
     2161 ttctcttcct ggtcctgcag ggatttcagc cagtacggca ctagtatcca tgtttttggt
     2221 gatctcctct agagcgttct ctgggatcat gtgttggtgt cccacaatgc agtgggccgc
     2281 aaggagtttg agcgagggca cttcaaacaa tgctgggtgg aacagaagtt ctctggctgt
     2341 tggtctgcga gcaggctcag actgcaggca cttttgaatg aactccctct gtaatgggtc
     2401 ttctagaagc tggatggcac tgctgatggc ttcctgtggc acatatgagg actctccatt
     2461 gccctgaatc tccagcactg ccatctccag tgcacacatg ccaaaggagt agatgtccac
     2521 tgctgttgtc acattagtga cttctccata ctctggtgca aagaagtgta gattcttctg
     2581 ctcttctcga caagtcttca catgattgtt gatagtgtca ggagccacag agccaatctt
     2641 gatgagtccg ttgtgctgga tgaagatggt gtcacaggtc aggttcccat ggatgatggg
     2701 ggggtcacag gagtgcaggt agcttagggc agagaggatt tgtgtgcacc aacgcttcca
     2761 tgccttttca ttcatcgtct tgtggttctt tttggtcttc ttcagaaatt gcttcagact
     2821 cccagatgac atgtattctg tgataaaaat gaccctggcc ttgttctctt taatgtcagc
     2881 ccaatatttg tgaaacttaa caatgttaag atgctccaat tgaatcagat tatcaaacac
     2941 agcacgaacc ttttcctcct gcagcttgta gttcttgcgt tcagagaact gtacctcatt
     3001 ccacacaacc tctacacctt cctctgtatc catggccagg tatgcactgt caatacctgg
     3061 tacattccgt tgattcacct cttctcgcct cttctgccag cgcccacagg gcgactcttc
     3121 caaaatctca gactcatctt cactttcttc ttcttcctct ggggaagcag ctgaggttgt
     3181 ggaggtcaca ggaggtgaca ctgatgtcag gccaggagct gaagatgagg attctacctt
     3241 tgggtctgag ccactgctaa gtactgtctg ggactccccc tccgacatgc tagcggatct
     3301 gacggttcac taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
     3361 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
     3421 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     3481 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
     3541 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     3601 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatagtcca
     3661 ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt ctacgtcacg
     3721 tattttgtac taatatcttt gtgtttacag tcaaattaat tccaattatc tctctaacag
     3781 ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctcg gtgaaggggg
     3841 cggccgctcg aggctagtct cgtgatcgat accgtcgaga tccgttcact aatcgaatgg
     3901 atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc
     3961 tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca
     4021 ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga
     4081 ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt
     4141 tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta
     4201 aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt
     4261 gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg
     4321 tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc
     4381 tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca
     4441 gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca
     4501 ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg
     4561 ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca
     4621 ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt
     4681 tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg
     4741 gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct
     4801 tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta
     4861 tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt
     4921 tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc
     4981 ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga
     5041 attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct
     5101 ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg
     5161 cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc
     5221 cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa
     5281 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     5341 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     5401 cccaggctca gatctggtct aaccagagag acccgtttat gtatcgagct aggcacttaa
     5461 atacaatatc tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc agacccgtct
     5521 gttgccttcc taataaggca cgatcgtacc accttacttc caccaatcgg catgcacggt
     5581 gctttttctc tccttgtaag gcatgttgct aactcatcgt taccatgttg caagactaca
     5641 agagtattgc ataagactac attaagcttg cagctccagc ttttgttccc tttagtgagg
     5701 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5761 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5821 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5881 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5941 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6001 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6061 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6121 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6301 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6541 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6781 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6841 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6901 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6961 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7021 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7081 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7141 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7201 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7261 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7321 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7381 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7441 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7501 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7561 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7621 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7681 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7741 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7801 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7861 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     7921 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     7981 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     8041 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     8101 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     8161 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     8221 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     8281 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     8341 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     8401 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     8461 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     8521 ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     8581 cagaggccga ggcggcctcg gcctctgcat aaat
//
