LOCUS       sequence-44833-e        7263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A positive control receptor bait with His-tag (rat CD4d3+4-HIS) and 
            prey (rat CD200) EXPRESs plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ
  TITLE     Large-scale screening for novel low-affinity extracellular protein 
            interactions.
  JOURNAL   Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.
  PUBMED    18296487
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2008-04"; volume: "18(4)"; pages: "622-30. Epub 2008 
            Feb 22"
COMMENT     Sequence Label: CD200CD4d3+4-His
FEATURES             Location/Qualifiers
     source          1..7263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             563..580
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    632..687
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(686..705)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2923..2941)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3009..3113
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3114..3182,3183..3974)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3332..3351)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4145..4733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4634..4653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4887..4904
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5021..5042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5057..5062,5063..5080,5081..5087)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5095..5111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5100..5122
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5119..5135
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5119..5135
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5406..5785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5786..5989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5940..5960
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5986..6010
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     6491..6510
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      6557..6566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact
       61 caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc
      121 ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac
      181 tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt
      241 ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta
      301 tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg
      361 tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga
      421 ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg
      481 gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc
      541 caaagggttg aattccggac cacatcatca ccatcaccac taagtctaga cacgtgatta
      601 attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt cattgcaata
      661 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcatttg
      721 gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga cgaactaaac
      781 ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt
      841 tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa
      901 aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg
      961 agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt
     1021 atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg
     1081 cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga
     1141 taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta
     1201 aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa
     1261 tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct
     1321 aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg
     1381 attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg
     1441 ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga
     1501 ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg
     1561 tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc
     1621 aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga
     1681 atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc
     1741 tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta
     1801 ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt
     1861 aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc
     1921 cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact
     1981 cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt
     2041 ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg
     2101 tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt
     2161 gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc
     2221 gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg
     2281 tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc
     2341 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     2401 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     2461 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     2521 ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt atatgctatc
     2581 ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat gctatcctaa
     2641 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa
     2701 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     2761 tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     2821 tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctca
     2881 tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg atacgcctat
     2941 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3001 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3061 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3121 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3181 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3241 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3301 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     3361 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3421 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3481 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3541 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3601 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     3661 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3721 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3781 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3841 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3901 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3961 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4021 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4081 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4141 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4201 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4261 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4321 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4381 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4441 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4501 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4561 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4621 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4681 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4741 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4801 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4861 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4921 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4981 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     5041 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     5101 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag
     5161 aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa cgttgttgcc
     5221 attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc aatattggca
     5281 attagccata ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca
     5341 tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc
     5401 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     5461 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     5521 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     5581 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     5641 acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc
     5701 cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta
     5761 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg
     5821 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     5881 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac
     5941 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     6001 ccgtcagatc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tgggctcgcg
     6061 gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc cgtcggcctc
     6121 cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga tcggaaaacc
     6181 tctcgagaaa ggcgtctaac cagtcacagt cgcaaggtag gctgagcacc gtggcgggcg
     6241 gcagcgggtg gcggtcgggg ttgtttctgg cggaggtgct gctgatgatg taattaaagt
     6301 aggcggtctt gagacggcgg atggtcgagg tgaggtgtgg caggcttgag atccagctgt
     6361 tggggtgagt actccctctc aaaagcgggc attacttctg cgctaagatt gtcagtttcc
     6421 aaaaacgagg aggatttgat attcacctgg cccgatctgg ccatacactt gagtgacaat
     6481 gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaagt ttaaactgcg
     6541 gccgctccgg ctagccgcca ccatggccag tccggtattc aggagacctt tctgccatct
     6601 gtccacctac agcctgctct gggccatagc agcagtagcg ctgagcacag ctcaagtgga
     6661 agtggtgacc caggatgaaa gaaagctgct gcacacaact gcatccttac gctgttctct
     6721 aaaaacaacc caggaaccct tgattgtgac atggcagaaa aagaaagccg taggcccaga
     6781 aaacatggtc acttacagca aagcccatgg ggttgtcatt cagcccacct acaaagacag
     6841 gataaacatc actgagctgg gactcttgaa cacaagcatc accttctgga acacaaccct
     6901 ggatgatgag ggttgctaca tgtgtctctt caacatgttt ggatctggga aggtctctgg
     6961 gacagcttgc cttactctct atgtacagcc catagtacac cttcactaca actattttga
     7021 agaccaccta aacatcacgt gctctgcaac tgcccgccca gcccctgcca tctcctggaa
     7081 gggcactggg tcaggaattg agaatagtac tgagagtcac tcccattcaa atgggactac
     7141 atctgtcacc agcatcctcc gggtcaaaga ccccaaaact caggttggaa aggaagtgat
     7201 ctgccaggtt ttatacttgg ggaatgtgat tgactacaag cagagtctgg acaaaggcgc
     7261 gcc
//
