LOCUS       sequence-448349-        6580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression mouse PTER protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Wei W, Lyu X, Markhard AL, Fu S, Mardjuki RE, Cavanagh PE, Zeng X, 
            Rajniak J, Lu N, Xiao S, Zhao M, Moya-Garzon MD, Truong SD, Chou JC,
            Wat LW, Chidambaranathan-Reghupaty S, Coassolo L, Xu D, Shen F, 
            Huang W, Ramirez CB, Jang C, Svensson KJ, Fischbach MA, Long JZ
  TITLE     A PTER-dependent pathway of taurine metabolism linked to energy 
            balance.
  JOURNAL   bioRxiv [Preprint]. 2024 Mar 22:2024.03.21.586194. doi: 
            10.1101/2024.03.21.586194.
  PUBMED    38562797
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Mar 22:2024.03.21.586194. doi: 
            10.1101/2024.03.21.586194."
COMMENT     Sequence Label: pDEST40-mPTER
FEATURES             Location/Qualifiers
     source          1..6580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1337..2131)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1448..1467)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2058..2077
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2198..2527)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2212..2347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2266..2285)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2512..2532
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2541..2969)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2651..2672)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2863..2882
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3015..3239
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3228..3245
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3268..3285)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3295..3336)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3362..3386
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4479..4503)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4532..4551)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4533..4551)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4596..4799)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4625..4645)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4800..5179
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5351..5370
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5445..5549
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5550..5618,5619..6410)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5768..5787)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgcg
     1321 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     1381 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     1441 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     1501 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     1561 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga
     1621 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     1681 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     1741 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     1801 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     1861 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     1921 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg
     1981 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     2041 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     2101 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     2161 cagatccgaa aatggatata caagctcccg ggagcttttt gcaaaagcct aggcctccaa
     2221 aaaagcctcc tcactacttc tggaatagct cagaggcaga ggcggcctcg gcctctgcat
     2281 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
     2341 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt
     2401 gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga
     2461 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact
     2521 gacacacatt ccacagaatt aattcgcgtt aaatttttgt taaatcagct cattttttaa
     2581 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     2641 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     2701 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     2761 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     2821 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     2881 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     2941 cgcgcttaat gcgccgctac agggcgcgtg gggatacccc ctagagcccc agctggttct
     3001 ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca
     3061 atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag
     3121 acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag
     3181 ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt
     3241 cgaggctgat cagcgggttt aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga
     3301 atcgagaccg aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagatc
     3361 aaccactttg tacaagaaag ctgggtcggc gcgcccaccc ttttttttaa aagtcagcca
     3421 ttgtttaggg ttttctatga gtatcttgtc aagcaccctc tcagtcagac ctctaaggag
     3481 catcttagga acaatgttgg taaggatgtg tgagtagccg tgacctccgt acttcatcaa
     3541 ccgatgcttt gtgtgtatgt catgtgccat gagaatccga tcttcatagc cctcatccac
     3601 tagaaaatgg acccttctaa ttcttttgtt atcatcaggc atatcaatat ctgggctcaa
     3661 ctggtaatta aggagttccg taccaaagag atcgtattcc aagtagcagc caagttgagc
     3721 aaactccagc agctctttct tatcaaatat agtcctgtca aggtgggaca tgactgtttt
     3781 ggagatgtct gctcctgctt cttgcagtat acggattatc tggaatggtg cacctgggtt
     3841 ccgtccagga tggatgatga caggacagcc aagctgagcc tgggcgtgag ctgtagcctc
     3901 aagtatcttt ctctcgctgt cagtcaaagg ccaggagcag ccaatttctc caataactcc
     3961 acacttgatg ctggtgccat cagctccatg gagaatttca ttaataagga catctgtaag
     4021 ctgctccact gacatggctc tggttgctgc agagtgagtt gcatcaacat aaaacccagc
     4081 tccagctatg atgtggactc cagtctgctc tgccagccac ttcagcgtat gcacgtccct
     4141 gctgagccca gttgtcgtat tctccaccaa ggctcctccg cccttagcct tgaaatacaa
     4201 cagctcttct cttatggctc ctacctcctg attcaactga aggttctctc gatgggaata
     4261 ggggtttttc tgaatccaaa atagattttt catcatgata ggttccttgg aggtgacttc
     4321 gtggcatgga ggaggtgggc agtaaaaact gtcaaaggtc attgtcagat gctcgtgggt
     4381 cagggtgcgt cccagttggc tgggttctac aaggcccaga actgtttgta ctttcccact
     4441 taaggaagac atggtggcgg tgaagggggc ggccgcggag cctgcttttt tgtacaaact
     4501 tgttgatagc ttaactagcc agcttgggtc tccctatagt gagtcgtatt aatttcgata
     4561 agccagtaag cagtgggttc tctagttagc cagagagctc tgcttatata gacctcccac
     4621 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
     4681 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga
     4741 aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc
     4801 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
     4861 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
     4921 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
     4981 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
     5041 gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta
     5101 acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa
     5161 ctagtcaata atcaatgtca acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa
     5221 cgcctaaccc taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct
     5281 taaattttgc tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg
     5341 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatagggg atcgggagat
     5401 ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5461 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5521 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5581 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5641 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5701 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5761 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5821 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5881 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5941 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6001 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6061 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6121 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6181 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6241 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6301 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6361 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6421 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6481 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6541 actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
