LOCUS       sequence-448360-        8943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression and inducible recruitment of protein in mammalian 
            cell culture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Adriaenssens E, Schaar S, Cook ASI, Stuke JFM, Sawa-Makarska J, 
            Nguyen TN, Ren X, Schuschnig M, Romanov J, Khuu G, Uoselis L, 
            Lazarou M, Hummer G, Hurley JH, Martens S
  TITLE     Reconstitution of BNIP3/NIX-mitophagy initiation reveals 
            hierarchical flexibility of the autophagy machinery.
  JOURNAL   Nat Cell Biol. 2025 Jul 25. doi: 10.1038/s41556-025-01712-y.
  PUBMED    40715440
REFERENCE   2  (bases 1 to 8943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 Jul 25. doi: 10.1038/s41556-025-01712-y."
COMMENT     Sequence Label: pHAGE-FKBP-mEGFP-WIPI4
FEATURES             Location/Qualifiers
     source          1..8943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2821..2830
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2827..2829,2830..3150)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             3157..3477
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3157..3477
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(3496..3498,3499..4209)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3538..3559)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3799..3818)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4146..4167
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5333..5921
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5386..5406)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5804..5815)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5996..6229
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6348..6366)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6434..6538
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6539..6607,6608..7399)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6757..6776)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7570..8158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8059..8078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8312..8329
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8569..8590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8605..8610,8611..8628,8629..8635)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8643..8659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8648..8670
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8667..8683
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8667..8683
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8733..8752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcggtacc
     2821 gccaccatgg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
     2881 cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat
     2941 tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
     3001 ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct
     3061 ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc
     3121 gtcttcgatg tggagcttct aaaactggaa actagaggcg tgcaggtcga gaccatcagc
     3181 cccggcgacg gccgcacctt tcccaagaga ggccagactt gcgtggtcca ctacaccggc
     3241 atgctggagg acggcaagaa gttcgacagc agccgcgacc gcaacaagcc cttcaagttc
     3301 atgctgggca aacaggaagt gatccgcggc tgggaggaag gcgtggctca gatgagcgtg
     3361 gggcagcggg ccaagctgac catcagcccc gactatgcct acggcgccac cggccacccc
     3421 ggcatcatcc ccccccacgc caccctggtg ttcgacgtgg agctgctgaa gctggagact
     3481 agtaccggtt ctagagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3541 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3601 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3661 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3721 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3781 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3841 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3901 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     3961 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4021 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4081 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     4141 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4201 ctgtacaagt ccggactcag atctcgagct caagcttcga attctactca acagccactt
     4261 cgaggagtga ccagcctgcg tttcaaccaa gaccaaagct gcttttgctg cgccatggag
     4321 acaggtgtgc gcatctacaa cgtggagccc ttgatggaga aggggcatct ggaccacgag
     4381 caggtgggca gcatgggctt ggtggagatg ctgcaccgct ccaaccttct ggccttggtg
     4441 ggcggtggta gtagtcccaa gttctcagag atctcagtgc tgatctggga cgatgcccgg
     4501 gagggcaagg actccaagga gaagctggtg ctggagttca ccttcaccaa gccagtgctt
     4561 tctgtgcgca tgcgccatga caagatcgtg atcgtgctga agaaccgcat ctatgtgtac
     4621 tccttccccg acaatccccg aaagctgttt gagtttgata cccgggacaa ccccaagggg
     4681 ctctgtgacc tctgccccag cctggagaag caactgctag tgttcccggg acacaagtgt
     4741 gggagtctgc aacttgtgga cctggcgagc acaaagcctg gcacctcgtc tgctccattc
     4801 acgatcaatg cacatcagag tgacatagcc tgtgtgtctc taaaccagcc aggcactgta
     4861 gtggcctcag cctcccagaa gggtaccctt attcgcctct ttgacacaca atccaaggag
     4921 aaactggtgg agctgcgccg aggcactgac cctgccaccc tctactgcat taacttcagc
     4981 cacgactcct ccttcctctg cgcttccagt gataagggta ctgtccatat ctttgctctc
     5041 aaggataccc gcctcaaccg ccgctccgcg ctggctcgcg tgggcaaggt ggggcctatg
     5101 attgggcagt acgtggactc tcagtggagc ctggcgagct tcactgtgcc tgctgagtca
     5161 gcttgcatct gcgccttcgg tcgcaatact tccaagaacg tcaactctgt cattgccatc
     5221 tgcgtagatg ggaccttcca caaatatgtc ttcactcctg atggaaactg caacagagag
     5281 gctttcgacg tgtaccttga catctgtgat gatgatgact tttaaggatc ctaatcaacc
     5341 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5401 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5461 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5521 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5581 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5641 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5701 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     5761 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5821 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5881 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca
     5941 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6001 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6061 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6121 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6181 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6241 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6301 cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg
     6361 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     6421 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6481 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6541 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6601 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6661 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6721 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6781 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6841 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6901 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6961 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7021 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7081 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7141 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7201 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7261 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7321 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7381 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7441 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7501 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7561 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7621 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7681 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7741 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7801 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7921 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7981 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8041 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8101 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8161 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8221 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8281 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8341 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct
     8401 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8461 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg
     8521 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8581 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8641 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac
     8701 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8761 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     8821 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     8881 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     8941 agt
//
