LOCUS       sequence-448377-        6381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing a fragment of gamma-tubulin containing gamma-tubulin 
            GTPase domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Lindstrom L, Alvarado-Kristensson M
  TITLE     Characterization of gamma-tubulin filaments in mammalian cells.
  JOURNAL   Biochim Biophys Acta. 2017 Oct 16. pii: S0167-4889(17)30283-5. doi: 
            10.1016/j.bbamcr.2017.10.008.
  PUBMED    29050966
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2017 Oct 16. pii: S0167-4889(17)30283-5. doi: 
            10.1016/j.bbamcr.2017.10.008."
COMMENT     Sequence Label: gamma-tubulin (1-335)
FEATURES             Location/Qualifiers
     source          1..6381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1965..1983)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1966..1983)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2003..2020)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2009..2233
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2279..2707
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2366..2385)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2576..2597
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2716..2736)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     2932..2951
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3086..3880
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3140..3159)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3750..3769
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4054..4175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4091..4110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4145..4164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4224..4240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4224..4240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4237..4259)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4248..4264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4272..4278,4279..4296,4297..4302))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4317..4338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4455..4472)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4626..5214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4706..4725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5385..6176,6177..6245))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6008..6027
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6246..6350)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttcgatgc
      901 cgagggaaat catcacccta cagctaggac aatgcggcaa tcagattggg ttcgagttct
      961 ggaaacagct gtgcgccgag catggtatca gccccgaggg catcgtggag gagttcgcca
     1021 ccgagggcac tgaccacaag gacgtctttt tctaccaggc agacgatgag cactacatcc
     1081 cccgggccgt gctgctggac ttggaacccc gggtgatcca ctccatcctc aactccccct
     1141 atgccaagct ctacaaccca gagaacatct acctgtcgga acatggagga ggagctggca
     1201 acaactgggc cagcggattc tcccagggag aaaagatcca tgaggacatt tttgacatca
     1261 tagaccggga ggcagatggt agtgacagtc tagagggctt tgtgctgtgt cactccattg
     1321 ctggggggac aggctctgga ctgggttcct acctcttaga acggctgaat gacaggtatc
     1381 ctaagaagct ggtgcagaca tactcagtgt ttcccaacca ggacgagatg agcgatgtgg
     1441 tggtccagcc ttacaattca ctcctcacac tcaagaggct gacgcagaat gcagactgtg
     1501 tggtggtgct ggacaacaca gccctgaacc ggattgccac agaccgcctg cacatccaga
     1561 acccatcctt ctcccagatc aaccagctgg tgtctaccat catgtcagcc agcaccacca
     1621 ccctgcgcta ccctggctac atgaacaatg acctcatcgg cctcatcgcc tcgctcattc
     1681 ccaccccacg gctccacttc ctcatgaccg gctacacccc tctcactacg gaccagtcag
     1741 tggccagcgt gaggaagacc acggtcctgg atgtcatgag gcggctgctg cagcccaaga
     1801 acgtgatggt gtccacaggc cgagaccgcc agaccaacca ctgctacatc gccatcctca
     1861 acatcatcca gggagaggtg gaccccaccc aggtccacaa gtaagaattc tgcagatatc
     1921 catcacactg gcggccgctc gagcatgcat ctagagggcc ctgttctata gtgtcaccta
     1981 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     2041 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     2101 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     2161 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     2221 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     2281 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2341 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2401 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2461 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2521 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2581 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2641 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2701 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2761 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2821 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2881 tcccgcccct aactccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     2941 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     3001 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     3061 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     3121 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3181 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3241 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     3301 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     3361 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     3421 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     3481 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     3541 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     3601 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     3661 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     3721 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     3781 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     3841 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     3901 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     3961 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     4021 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     4081 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4141 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     4201 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4261 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4321 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4381 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4441 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4501 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4561 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4621 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4681 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4741 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4801 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4861 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4921 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4981 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5041 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     5101 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5161 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5221 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5281 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5341 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5401 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5461 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5521 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5581 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5641 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5701 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5761 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5821 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5881 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5941 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6001 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6061 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6121 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6181 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6241 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6301 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6361 cgaaaagtgc cacctgacgt c
//
