LOCUS       sequence-448387-        5829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan-neuronal, pan-membrane expression of the genetically encoded 
            voltage indicator ASAP5; can be used for dendritic voltage imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Hao YA, Lee S, Roth RH, Natale S, Gomez L, Taxidis J, O'Neill PS, 
            Villette V, Bradley J, Wang Z, Jiang D, Zhang G, Sheng M, Lu D, 
            Boyden E, Delvendahl I, Golshani P, Wernig M, Feldman DE, Ji N, Ding
            J, Sudhof TC, Clandinin TR, Lin MZ
  TITLE     A fast and responsive voltage indicator with enhanced sensitivity 
            for unitary synaptic events.
  JOURNAL   Neuron. 2024 Sep 17:S0896-6273(24)00643-3. doi: 
            10.1016/j.neuron.2024.08.019.
  PUBMED    39305894
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2024 Sep 17:S0896-6273(24)00643-3. doi: 
            10.1016/j.neuron.2024.08.019."
COMMENT     Sequence Label: pAAV-hsyn-ASAP5-WPRE
FEATURES             Location/Qualifiers
     source          1..5829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      642..651
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1956..2544
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2009..2029)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2427..2438)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2576..3052
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2775..2794)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3092..3232
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3092..3221
                     /label=AAV2 ITR
     rep_origin      3307..3762
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3394..3413)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3604..3625
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3779..3798)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3898..3920
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3958..3976)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4044..4148
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4149..4217,4218..5009)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4367..4386)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5180..5768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5669..5688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc cgccaccatg gagacgactg
      661 tgaggtatga acaggggtca gagctcacta aaacttcgag ctctccaaca gcagatgagc
      721 ccacgataaa gattgatgat ggtcgtgatg agggtaatga acaagacagc tgttccaata
      781 ccattaggag aaaaatttcc ccgtttgtga tgtcatttgg attcagagta tttggagttg
      841 tgcttatcat tgtagacatc atagtggtga ttgtggatct ggccatcagt gagaagaaaa
      901 gaggcattag agagattctt gaaggtgttt ccctggctat agcactcttc ttccttgttg
      961 atgttctcat gagagtgttt gttgaaggct tcaagaacta tttccggtcc aaactgaata
     1021 ctttggatgc agtcatagta gtgggcactc tgctaattaa tatgacctac tccttctctg
     1081 ctggtacatt taaaggtgat aacgtgtata ttaccgcgga taaacagaaa aacggcatta
     1141 aagcgaactt taccgtgcgc cataacgtgg aagatggcag cgtgcagctg gcggatcatt
     1201 atcagcagaa caccccgatt ggcgatggcc cggtgctgct gccggataac cattatctga
     1261 gcacccagac cgtgctgagc aaagatccga acgaaaaacg cgatcacatg gtgctgctgg
     1321 aatttgtgac cgcagcgggc attacacacg gcatggatga actgtatggc ggcaccggcg
     1381 gcagcgcgag ccagggcgaa gaactgttta ccggcgtggt gccgattctg gtggaactgg
     1441 atggcgatgt gaacggccat aaatttagcg tgcgcggcga aggcgaaggc gatgcgacca
     1501 ttggcaaact gaccctgaaa tttatttgca ccaccggcaa actaccggtg ccgtggccga
     1561 ccctggtgac caccttaacc tatggcgtgc agtgctttag ccgctatccg gatcatatga
     1621 aacgccatga tttttttaaa agcgcgatgc cggaaggcta tgtgcaggaa cgcaccatta
     1681 gctttaaaga tgatggcaaa tataaaaccc gcgcggtggt gaaatttgaa ggcgataccc
     1741 tggtgaaccg cattgaactg aaaggcaccg attttaaaga agatggcaac attctggggc
     1801 ataaactgga atataacaca gatcagatgc cgcagatggt tactcttctt cgagttctga
     1861 gaattgttat cttaataaga gtgattcgcc tggcttcaca gaagaaacaa cttgaagtgg
     1921 taacctgata agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     1981 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     2041 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     2101 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     2161 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     2221 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     2281 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     2341 tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc
     2401 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     2461 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     2521 tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg
     2581 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
     2641 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
     2701 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg
     2761 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac
     2821 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg
     2881 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt
     2941 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc
     3001 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt
     3061 gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     3121 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     3181 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     3241 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     3301 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3361 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
     3421 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3481 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     3541 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3601 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
     3661 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
     3721 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     3781 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     3841 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     3901 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     3961 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4021 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4081 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4141 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4201 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4261 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4321 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4381 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4441 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4501 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4561 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4621 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4681 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4741 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4801 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4861 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4921 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4981 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5041 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5101 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5161 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5221 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5281 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5341 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5401 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5461 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5521 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5581 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5641 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5701 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5761 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5821 ctcacatgt
//
