LOCUS       sequence-44839-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Semple JI, Biondini L, Lehner B
  TITLE     Generating transgenic nematodes by bombardment and antibiotic 
            selection.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864.
  PUBMED    22290182
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "118-9"
COMMENT     Sequence Label: pBCN39-R4R3
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(294..314)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 atccatcttg ttcaatcatc gtttaccctg aaataaatac tgataagaag gggaaagttt
       61 agaaatacac gagagtaaag gaagagagat aaatcgaagc gagagaacgc gcataacaat
      121 aaatatatga aaactatttc gagaatttat ttggcaagaa tcccgcacaa attcttctaa
      181 tatttacata tttcagtaat cgctagctca tcaggcaccg ggcttgcggg tcatgcacca
      241 ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta
      301 gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc
      361 cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt gctgaaaatt
      421 taaatcagat tagttagtta gtaaaatcat gtttaaactt acgtcgggcg agacgccgac
      481 ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg
      541 ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa
      601 caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc
      661 cgctgaaaat ttaaataatc agggttagtt agtatatata tgtttaaact taccgaccca
      721 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg
      781 ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc
      841 ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg gcgag
//
